Abstract:
:Effects of amino acid substitutions in the first extracellular loop region of the delta- and mu-opioid receptors were examined. Substitution of lysine-108 of the delta-receptor (delta K108) with asparagine improved affinity to [D-Ala2,MePhe4,Gly-ol5]enk ephalin (DAGO), a mu-selective peptide agonist, to be comparable with that of the mu-receptor. On the other hand, replacement of mN127 with lysine decreased the affinity to DAGO by approximately 15-fold. These results suggest that dK108 and mN127, which correspond to each other in the aligned amino acid sequences, mainly determine the difference in DAGO binding affinity between the delta- and mu-receptors.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Fukuda K,Terasako K,Kato S,Mori Kdoi
10.1016/0014-5793(95)01034-csubject
Has Abstractpub_date
1995-10-09 00:00:00pages
177-81issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(95)01034-Cjournal_volume
373pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Glycogen synthase from human and bovine polymorphonuclear leukocytes was purified to homogeneity. Rabbit antisera were raised against the two glycogen synthases and used for immunochemical analysis. Western blotting analysis showed that the subunit of glycogen synthase in crude homogenates of human and bovine leukocyt...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80653-7
更新日期:1988-03-28 00:00:00
abstract::The pre-steady state process in the thermolysin-catalyzed hydrolysis of Cbz-Gly-Phe-Ala was observed at pH 4.5 by fluorescence stopped-flow method using Dns-Phe as a displacement probe. After the confirmation of the pre-equilibrium hypothesis for the binary interaction, the nonlinear substrate concentration dependence...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00242-2
更新日期:1995-04-03 00:00:00
abstract::The molecular basis and functional properties of a variant antithrombin (AT) protein. AT Budapest 3, were studied. A single base substitution was identified in codon 99, CTC----TTC, altering the normal leucine to phenylalanine. The proband presented with a history of venous thrombotic disease and was found to be homoz...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80854-a
更新日期:1992-04-06 00:00:00
abstract::The tomato saponin alpha-tomatine has been proposed to kill sensitive cells by binding to cell membranes followed by leakage of cell components. However, details of the modes of action of the compound on fungal cells are poorly understood. In the present study, mechanisms involved in alpha-tomatine-induced cell death ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.06.010
更新日期:2007-07-10 00:00:00
abstract::Prefibrillar cytotoxicity was suggested as a common amyloid characteristic. We showed two types of albebetin prefibrillar oligomers are formed during incubation at pH 7.3. Initial round-shaped oligomers consist of 10-15 molecules determined by atomic force microscopy, do not bind thioflavin-T and do not affect viabili...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.03.074
更新日期:2006-05-01 00:00:00
abstract::Plastoquinone-9 (PQ-9)-depleted PSII reaction center core complex, consisting of CP47/D1/D2/Cytb-559/I, was isolated from spinach PSII particles. PQ-9, lipids and several proteins were extracted from the original PSII particles and separated by several steps of chromatography to be reconstituted into the isolated comp...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01089-7
更新日期:1994-10-31 00:00:00
abstract::Liver microsomes contain a vitamin K and O2-dependent carboxylase that converts peptide-bound glutamyl residues to gamma-carboxyglutamate residues. The peptide Boc-O-phospho-Ser-O-phospho-Ser-Leu-OMe has now been synthesized. This peptide inhibits the carboxylation of endogenous protein precursors by a detergent-solub...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80486-4
更新日期:1983-02-07 00:00:00
abstract::Antibodies were made to a thirteen amino acid synthetic peptide corresponding to the C-terminal portion of the glutamate (glu) receptor, GluR-A. The immunoprecipitation of kainic acid (KA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) binding sites by the anti-peptide antibodies was studied using...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80529-r
更新日期:1990-12-10 00:00:00
abstract::The morphology of complexes between relaxed circular plasmid pBR322 DNA and tripeptide L-Val-L-Val-L-Val-NH-NH-Dns (TVP) at different peptide/DNA ratios was studied by electron microscopy. The results show that interaction of TVP with circular DNA leads to the formation of perfect torus-shaped particles. The torus par...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80236-2
更新日期:1985-01-21 00:00:00
abstract::The Pb, Eu and Mg-induced cleavages in E. coli and lupine tRNAPhe have been characterized and compared with those found in yeast tRNAPhe. The pattern of lupine tRNAPhe hydrolysis closely resembles that of yeast tRNAPhe, while several major differences occur in the specificity and efficiency of the E. coli tRNAPhe hydr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80148-6
更新日期:1989-01-30 00:00:00
abstract::The ubiquitin-editing enzyme A20 suppresses nuclear factor-κB (NF-κB) activation and tumor necrosis factor-α (TNF-α)-induced apoptosis in a deubiquitinating and ubiquitin ligase activity-dependent manner. Although recent studies revealed that A20 regulates NF-κB independently of its enzymatic activity through its seve...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.04.022
更新日期:2015-05-22 00:00:00
abstract::Activation of AMP-activated protein kinase (AMPK) has been viewed as an important target for the treatment of insulin resistance. Here, by proteomic analysis, we found that expression of heat shock protein beta-1 (HSPB1) was induced by the AMP analog 5-aminoimidazole-4-carboxamide 1-β-D-ribofuranoside in palmitate-ind...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12516
更新日期:2017-01-01 00:00:00
abstract::The gene trap methodology is a powerful tool to characterize novel genes and analyze their importance in biological phenomena. It is based on the use of mouse embryonic stem cells and reporter vectors designed to randomly integrate into the genome, tagging an insertion site and generating a mutation. Theoretically, al...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(00)01779-8
更新日期:2000-08-25 00:00:00
abstract::The differential scanning microcalorimetry and fluorescence methods, using probes ANS and pyrene, have been employed to study thermotropic behaviour of rat liver microsomes in the presence and absence of Mg2+. Addition of Mg2+ yields three partially reversible phase transitions at 18, 27 and 32 degrees C, respectively...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80295-6
更新日期:1983-12-12 00:00:00
abstract::Evolutionary trees based on partial small ribosomal subunit RNA sequences of 22 metazoa species have been published [(1988) Science 239, 748-753]. In these trees, cnidarians (Radiata) seemed to have evolved independently from the Bilateria, which is in contradiction with the general evolutionary view. In order to furt...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81212-7
更新日期:1990-09-03 00:00:00
abstract::A new inositol derivative could be isolated from the Archaeum Pyrococcus woesei and identified as di-myo-inositol-1,1'-phosphate by 1H, 31P NMR spectroscopy, mass spectrometry and thin layer chromatography. In P. woesei, this inositol phosphate represents the dominant counterion of K+ which ranges from 500 to 600 mM. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81008-a
更新日期:1992-07-20 00:00:00
abstract::Antithrombin III Hamilton is a structural variant of antithrombin III (AT-III) with normal heparin affinity but impaired serine protease inhibitory activity. The molecular defect of AT-III-Hamilton is a substitution of threonine for alanine at amino acid residue 382. Recently it has been shown that both plasma-derived...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80305-m
更新日期:1991-03-25 00:00:00
abstract::Mitochondrial thioredoxin (mtTrx) can be oxidized in response to inducers of oxidative stress; yet the functional consequences of the oxidation have not been determined. This study evaluated the redox status of mtTrx and its association to oxidant-induced apoptosis. Results showed that mtTrx was oxidized after exposur...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.11.007
更新日期:2006-12-11 00:00:00
abstract::Syntenin has crucial roles in cell adhesion, cell migration and synaptic transmission. Its closely linked postsynaptic density-95, discs large 1, zonula occludens-1 (PDZ) domains typically interact with C-terminal ligands. We profile syntenin PDZ1-2 through proteomic peptide phage display (ProP-PD) using a library tha...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12037
更新日期:2016-01-01 00:00:00
abstract::Truncated cyclin A and cyclin B lacking the N-terminal domain comprising the 'destruction box' escape from proteolysis and arrest cells at metaphase. Mutation of a conserved arginine residue of the destruction domain makes cyclin B resistant to proteolysis. Here we show that mutation of the same residue also makes cyc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80844-7
更新日期:1992-07-13 00:00:00
abstract::We recently purified two closely related 33 kDa proteins from rat hepatic cytosol, designated bile acid binder I and II, which selectively bind bile acids with comparable affinity as glutathione S-transferase B. This work has now been extended to human liver in which we have identified a similar cytosolic binding acti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80975-8
更新日期:1984-11-05 00:00:00
abstract::The Ca(2+)-calmodulin dependent protein phosphatase, calcineurin, is thought to mediate the action of the two immunosuppressants, cyclosporin A (CsA) and FK506. Calcineurin from all species consists of a catalytic A subunit and a regulatory peptide B, which plays an essential role in catalysis. The enzymatic function ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01365-8
更新日期:1995-01-03 00:00:00
abstract::The Methanocaldococcus jannaschii (formerly Methanococcus jannaschii) protein Mj0968 has been reported to represent a soluble P-type ATPase [Ogawa et al., FEBS Lett. 471 (2000) 99-102]. In this study, we report that the heterologously expressed Mj0968-His(10) protein exhibits high rates of phosphatase activity, wherea...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00372-7
更新日期:2003-05-22 00:00:00
abstract::The carbohydrate binding properties of jacalin lectin were examined using RAF9 cell-derived D-[6-3H]glucosamine-radiolabeled total glycopeptides containing N-linked and O-linked oligosaccharides. The binding of N-linked glycopeptides to jacalin was abolished by treatment of alpha-galactosidase whereas O-linked glycope...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01539-1
更新日期:1998-01-09 00:00:00
abstract::Although it has been shown that phosphorylations of p53 serine its residues are critical events for the regulation of their function, the specific biological effects of each of these phosphorylations, especially at serine 392, remain to be elucidated. Serine 392 has been proposed to play a role in the tetramerization ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.07.014
更新日期:2004-08-13 00:00:00
abstract::Previously, we found that intracellular reactive oxygen species (ROS) affect photomorphogenesis in Neurospora crassa. In this study, we investigated the physiological roles of ROS in the response to light and found that the exposure of mycelia to air was important for the light-induced carotenogenesis. Mycelia treated...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.06.014
更新日期:2005-07-18 00:00:00
abstract::We report a new method for the detection of ligand-induced receptor internalization by fluorescence ratiometric imaging of pH in endosomes in combination with a recently developed posttranslational labeling system based on the formation of a heterodimeric coiled-coil structure. The N-terminus of the β2-adrenergic rece...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.06.012
更新日期:2011-07-21 00:00:00
abstract::The 23-residue synthetic peptide representing the N-terminus of HIV-1 gp41 is known to induce either leakage or fusion of lipid vesicles depending on the experimental conditions. In this paper we report that a polar amino acid substitution V-->E at position 2, known to block gp41 activity in vivo, makes the peptide un...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00257-a
更新日期:1995-04-03 00:00:00
abstract::Human high- and low-Mr kininogens were shown to be potent inhibitors of cysteine proteinases such as cathepsin L and papain (Ki = 17-48 pM). A strong immunological cross-reaction between the kininogens and low-Mr alpha-cysteine proteinase inhibitor from human plasma was found. Comparison of partial amino acid sequence...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80322-7
更新日期:1985-03-25 00:00:00
abstract::A 3.3 kb cDNA encoding the complete amino acid sequence of a calcium/calmodulin regulated protein phosphatase has been isolated from a Drosophila eye disc cDNA library. The predicted protein of 560 amino acids (molecular mass 62 kDa) is 73-78% identical to human PP2B isoforms. The cDNA hybridised to the X-chromosome a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80398-6
更新日期:1994-02-14 00:00:00