Abstract:
:Glycogen synthase from human and bovine polymorphonuclear leukocytes was purified to homogeneity. Rabbit antisera were raised against the two glycogen synthases and used for immunochemical analysis. Western blotting analysis showed that the subunit of glycogen synthase in crude homogenates of human and bovine leukocytes in both cases has an Mr of 85,000. The existence of a cross-reactivity between the two enzymes and the corresponding antisera demonstrates immunological similarities between bovine and human leukocyte glycogen synthase. In addition, both antisera recognize glycogen synthase in crude cellular extracts from rabbit and rat liver and from skeletal muscle. Leukocyte glycogen synthase, therefore, cannot be classified as either muscle (M-type) or liver (L-type) glycogen synthase and our results do not support the proposed immunochemical distinction between M- and L-type glycogen synthase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Rasmussen LH,Juhl H,Esmann V,Petersen HUdoi
10.1016/0014-5793(88)80653-7subject
Has Abstractpub_date
1988-03-28 00:00:00pages
116-20issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(88)80653-7journal_volume
230pub_type
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