Abstract:
:The Methanocaldococcus jannaschii (formerly Methanococcus jannaschii) protein Mj0968 has been reported to represent a soluble P-type ATPase [Ogawa et al., FEBS Lett. 471 (2000) 99-102]. In this study, we report that the heterologously expressed Mj0968-His(10) protein exhibits high rates of phosphatase activity, whereas only very low ATPase activity was measured. Replacement of the aspartate residue in the DSAGT motif (D7A), which becomes phosphorylated during the reaction cycle of P-type ATPases, does not affect the V(max), but only the K(M) of the reaction. Labeling studies with [gamma-(32)P]ATP and [alpha-(32)P]ATP revealed that the previously reported labeling experiments [Ogawa et al., 2000] do not necessarily show phosphorylation of Mj0968, but rather point to ATP binding. Binding studies with trinitrophenyl adenosine nucleotides showed low apparent K(d) values for those molecules. These results provide evidence that the native function of Mj0968 seems to be that of a phosphatase, rather than that of an ATP-hydrolyzing enzyme.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Bramkamp M,Gassel M,Herkenhoff-Hesselmann B,Bertrand J,Altendorf Kdoi
10.1016/s0014-5793(03)00372-7keywords:
subject
Has Abstractpub_date
2003-05-22 00:00:00pages
31-6issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579303003727journal_volume
543pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::We here cloned a cDNA encoding STAM2, a new member of the STAM family, which contains an SH3 domain and ITAM. STAM2 like STAM1 is associated with Jak2 and Jak3, and involved in the signaling for DNA synthesis and c-myc induction mediated by IL-2 and GM-CSF. Co-expression of the SH3 deletion mutants of STAM1 and STAM2 ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01760-9
更新日期:2000-07-14 00:00:00
abstract::A 32 kDa phospholipase A2 inhibitory protein was isolated from pig thyroid gland after calcium precipitation and fast protein liquid anion-exchange chromatography. SDS-polyacrylamide gel electrophoresis revealed the purity of the protein. The protein activity was assessed by the inhibition of pancreatic phospholipase ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81273-0
更新日期:1988-08-01 00:00:00
abstract::Guluronate and mannuronate oligomers with various degree of polymerization were prepared from polyguluronate (PG) and polymannuronate (PM) with an alginate lyase from a Pseudoalteromonas sp., and their activities to induce cytokine secretion from mouse macrophage cell line RAW264.7 cells were examined. Enzymatically d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.07.007
更新日期:2005-08-15 00:00:00
abstract::RNA-binding protein kinase from amphibian oocytes modifies serine and threonine residues in the molecules of substrates and utilizes both ATP and GTP. Low concentrations of heparin inhibit protein kinase. The foregoing suggests that this enzyme is casein kinase II. It is shown that RNA-binding proteins lack active for...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81363-0
更新日期:1984-05-07 00:00:00
abstract::Cell-substratum adhesion of rat hepatocytes was inhibited by antisera raised against plasma membranes of liver (anti-liver antiserum) and Morris hepatoma 7777 (anti-hepatoma antiserum). Similar concentrations of both antisera inhibited adhesion on collagen. Anti-liver antiserum also inhibited the adhesion of hepatocyt...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80254-9
更新日期:1984-03-26 00:00:00
abstract::The full-length cDNA encoding the human calbindin-D9k (CaBP-9k) has been cloned using reverse transcription/PCR methodology with rat- and bovine-derived primers and intestinal RNA. A core product, and both a 5' and 3' product encompassing the full-length cDNA were obtained. The clones include coding region for 79 amin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80772-9
更新日期:1992-07-28 00:00:00
abstract::The mixed lineage kinase (MLK) family is a recently described protein kinase family. The MLKs contain a kinase domain followed by a dual leucine zipper-like motif. We previously reported the molecular cloning of LZK (leucine zipper-bearing kinase), a novel MLK, and that LZK activated the c-Jun NH2 terminal kinase (JNK...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02432-7
更新日期:2001-01-19 00:00:00
abstract::TATA-box binding factor TFIID is one of the key factors in transcriptional activation. Surprisingly, the yeast TFDII protein [(1989) Nature 341, 299-303; (1989) Cell 56, 1173-1181; (1989) Proc. Natl. Acad. Sci USA 86, 7785-7789] reveals only limited homology with other DNA-binding proteins. From computer-assisted sear...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(90)80558-z
更新日期:1990-02-26 00:00:00
abstract::Replication protein A (RP-A) was isolated from tobacco suspension cells and purified to near homogeneity by a procedure involving isolation of protoplasts, preparation of nuclei, nuclear lysis, binding to a column of single-stranded (ss) DNA cellulose and elution at different salt concentrations. The purified protein ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00897-1
更新日期:1997-08-11 00:00:00
abstract::A high intake of dietary fat has been suggested to diminish mitochondrial functioning in skeletal muscle, possibly attributing to muscular fat accumulation. Here we show however, that an 8-week high-fat dietary intervention did not affect intrinsic functioning of rat skeletal muscle mitochondria assessed by respiromet...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.01.013
更新日期:2008-02-20 00:00:00
abstract::Lysine-rich phosphorylated cystatin alpha (P-cystatin alpha) from newborn rat epidermis is a good substrate for epidermal transglutaminase (TGase) and also one of the component proteins of cornified envelope in the stratum corneum. Since the filaggrin linker segment peptide was efficiently conjugated with P-cystatin a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80131-2
更新日期:1994-03-07 00:00:00
abstract::We have purified a form of protein phosphatase 1 (PP1) from HeLa cell nuclei, in which the phosphatase is complexed to a regulatory subunit termed p99. We report here the cloning and characterisation of the p99 component. p99 mRNA is widely expressed in human tissues and immunofluorescence analysis with anti-p99 antib...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01485-3
更新日期:1997-12-22 00:00:00
abstract::Tert-butyl hydroperoxide (BHP), hydrogen peroxide and diamide caused a rapid and simultaneous release of glutathione disulfide (GSSG) and K+ in the isolated perfused rat liver. Both BHP-induced effluxes were suppressed by prior depletion of hepatic glutathione, but not by co-infusion of desferrioxamine which prevented...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81692-s
更新日期:1993-11-08 00:00:00
abstract::The phytocannabinoid Delta(9)-Tetrahydrocannabinol (Delta(9)-THC), the main psychoactive cannabinoid in cannabis, activates a number of signalling cascades including p53. This study examines the role of Delta(9)-THC in regulating the p53 post-translational modifier proteins, Murine double minute (Mdm2) and Small Ubqui...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.09.056
更新日期:2009-11-03 00:00:00
abstract::Tissue plasminogen activator was purified in high yield from pig heart by immunoaffinity chromatography and characterized by analysis of the glycosylation pattern and the N-terminal amino acid sequence. Comparisons with the human enzyme reveals residue exchanges in the A-chain at positions 3 (porcine Arg/human Gln) an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80872-9
更新日期:1986-09-01 00:00:00
abstract::Local bending propensity and curvature of DNA can be characterized using a vector description of DNA bendability, based on a set of parameters derived from deoxyribonuclease I (DNase I) cleavage experiments. Two characteristics-arithmetic and vector averages of bendability-were successfully used to predict experimenta...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00837-x
更新日期:1996-09-09 00:00:00
abstract::A microsomal fraction prepared from the mycelia of the band (bd) strain of Neurospora crassa showed enhanced phosphorylation of two small proteins with molecular masses of around 15 kDa (ps15) by the irradiation of the reaction mixture containing [gamma-32P]ATP at 0 degrees C for 1 s with blue light (450 nm, 6 mumol/m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00436-6
更新日期:1994-05-30 00:00:00
abstract::The chemokine RANTES is a potent chemoattractant for eosinophils. RANTES is produced by lung epithelial cells during eosinophil-rich inflammatory diseases such as asthma. In this study, we examined the effects of thiazolidinediones (TZD) on RANTES expression in a human lung epithelial cell line, A549. In A549 cells, i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00678-x
更新日期:1999-06-11 00:00:00
abstract::Nitrogen dioxide (NO2.) is often present in inhaled air and may be generated in vivo from nitric oxide. Exposure of human blood plasma to NO2. caused rapid losses of ascorbic acid, uric acid and protein thiol groups, as well as lipid peroxidation and depletions of alpha-tocopherol, bilirubin and ubiquinol-10. No incre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81185-o
更新日期:1992-11-16 00:00:00
abstract::In this review, we summarize the currently available information on the membrane topology of some key members of the human ABC protein subfamilies, and present the predicted domain arrangements. In the lack of high-resolution structures for eukaryotic ABC transporters this topology is based only on prediction algorith...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2005.11.040
更新日期:2006-02-13 00:00:00
abstract::The gene for human tetranectin was isolated from a genomic library with a mixture of degenerate oligonucleotide probes. The gene is about 12 kbp and contains two intervening sequences. The gene encodes a protein of 202 amino acid residues, with a signal peptide of 21 amino acid residues, followed by the tetranectin se...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80729-z
更新日期:1992-08-31 00:00:00
abstract::Hypo-osmotic swelling of human Intestine 407 cells leads to a significant increase of intracellular MAPKAP-kinase 2 activity and Hsp27 phosphorylation. Pre-treatment of the cells with the p38 MAP kinase inhibitor SB-203580 blocks this activation, indicating that the hypotonicity-induced activation of MAPKAP kinase 2 i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)01028-9
更新日期:1996-10-21 00:00:00
abstract::Bovine insulin can be split into several fractions by gel filtration on Sephadex G50. These are, in order of decreasing molecular weight, a new unidentified substance, proinsulin, and a large fraction containing several substances. Ultracentrifugal examination of the proinsulin in acetic acid solution (pH 2.3) gave a ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(68)80122-x
更新日期:1968-12-01 00:00:00
abstract::The protein kinase C activator, phorbol-12-myristate-13-acetate (PMA), augments the cyclic AMP accumulation induced by forskolin in pheochromocytoma (PC 12) cells with an EC50 value of 14 nM, while having no effect on basal values. At a concentration of 100 nM PMA markedly augmented the magnitude of the forskolin resp...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80227-7
更新日期:1986-02-03 00:00:00
abstract::The dependence of protein splicing on conserved residues of the Cne PRP8 intein was assessed by alanine scanning mutagenesis in a foreign protein context. Corroboration was obtained for the involvement of residues at the splice junctions and of the conserved threonine and histidine of motif B. Five additional residues...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.05.060
更新日期:2007-06-26 00:00:00
abstract::The intracellular distribution of DNA methyltransferase has been analyzed in synchronously proliferating human cells. The localization of DNA methyltransferase was determined immunocytochemically using monoclonal antibodies directed against this enzyme. DNA methyltransferase was found to accumulate predominantly in nu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80275-8
更新日期:1988-08-15 00:00:00
abstract::Glutamate uptake by rat hepatocytes in primary monolayer culture was found to be mediated by a Na+-independent and by two Na+-dependent transport systems of high and low affinity. Inhibition studies with cysteate and other model amino acids rules out the participation of the neutral amino acid transport systems A, ASC...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81024-2
更新日期:1983-09-19 00:00:00
abstract::Phosphoenolpyruvate carboxylases from various organisms contain two conserved lysine residues. In the C4 dicot Flaveria trinervia, one of these residues is Lys600. Converting this Lys600 to Arg600 or Thr600 mainly increased the Km values and but had minimal effect on the Vmax. The Km for PEP, Mg2+ increased by up to 3...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01189-l
更新日期:1995-11-13 00:00:00
abstract::Structures and regulations of vertebrate channels responsible for sustained calcium elevations after hormone stimulation are largely unknown. Therefore, the Drosophila photoreceptor channels, trp and trpl, which are assumed to be involved in calcium influx, serve as model system, trpl expressed in Sf9 cells showed spo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01455-a
更新日期:1995-01-30 00:00:00
abstract::Human prothymosin alpha mutants were generated with the aid of random mutagenesis and screened for their ability to inhibit yeast Saccharomyces cerevisiae cell growth. Conversion of Lys-87 to Glu resulted in an inactivated prothymosin alpha mutant, which lost the ability of the wild-type protein to block yeast cell gr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01171-4
更新日期:1996-11-18 00:00:00