Light signals are transduced to the phosphorylation of 15 kDa proteins in Neurospora crassa.

abstract：:Horse plasma gelsolin labelled with benzophenone-4-isothiocyanate can be photochemically cross-linked to rabbit cardiac tropomyosin. The cross-linking proceeds with greater efficiency in calcium-containing buffers. Further evidence for interaction between these proteins is provided by retention of fluorescently labell...

journal_title：FEBS letters

authors： Koepf EK,Burtnick LD

更新日期：1992-08-31 00:00:00

abstract：:A native female-specific chemoreceptive protein of a swallowtail butterfly [oviposition stimulant binding protein (OSBP)] was shown to specifically bind to aristolochic acid, a main stimulant for oviposition from its host plant. Oviposition stimulants are recognized by chemoreceptive organs of insects. OSBP isolated p...

journal_title：FEBS letters

authors： Tsuchihara K,Wazawa T,Ishii Y,Yanagida T,Nishida R,Zheng XG,Ishiguro M,Yoshihara K,Hisatomi O,Tokunaga F

更新日期：2009-01-22 00:00:00

abstract：:The ATPase Cdc48 is required for membrane fusion and protein degradation. Recently it has been suggested that Cdc48 in a complex with Ufd1 and Npl4 acts as an ubiquitin-dependent chaperone. Here it is shown that recombinant Cdc48 alone can distinguish between the native and the non-native conformation of model substra...

journal_title：FEBS letters

authors： Thoms S

更新日期：2002-06-05 00:00:00

abstract：:The trk family of tyrosine protein kinase genes serves crucial roles for the development of the nervous system and the survival of neurons. The members of this gene family, trk, trkB and trkC, bind a distinct neurotrophin of the nerve growth factor (NGF) gene family, and trigger the intracellular signals which elicit ...

journal_title：FEBS letters

authors： Okazawa H,Kamei M,Kanazawa I

更新日期：1993-08-23 00:00:00

abstract：:Transcriptional responses to growth in high environmental calcium concentrations were characterized and compared between wild-type and mutant Arabidopsis plants containing a knockout mutation in the gene encoding a cyclic nucleotide-gated channel (CNGC2). We show that the transcriptional profile of cngc2 plants grown ...

journal_title：FEBS letters

authors： Chan CW,Wohlbach DJ,Rodesch MJ,Sussman MR

更新日期：2008-03-19 00:00:00

abstract：:The molecular chaperone protein Hsp78, a member of the Clp/Hsp100 family localized in the mitochondria of Saccharomyces cerevisiae, is required for maintenance of mitochondrial functions under heat stress. To characterize the biochemical mechanisms of Hsp78 function, Hsp78 was purified to homogeneity and its role in t...

journal_title：FEBS letters

authors： Krzewska J,Langer T,Liberek K

更新日期：2001-01-26 00:00:00

abstract：:We have identified and purified a DNA binding protein which specifically binds to a segment of the mouse alpha 2 (I) collagen promoter between -420 and -399 bp upstream of the start of transcription. Purification included heparin-agarose and sequence-specific DNA-affinity chromatography, followed by SDS-PAGE and renat...

journal_title：FEBS letters

authors： Hatamochi A,de Crombrugghe B,Krieg T

更新日期：1993-08-02 00:00:00

abstract：:We show here that phosphorylation of the insulin receptor and insulin receptor substrate-1 is increased when suspended cells are replated on fibronectin. This is not due to decreased numbers of cell surface receptors, alteration of insulin binding, or stimulation of a phosphatase activity in non-adherent cells. Expres...

journal_title：FEBS letters

authors： El Annabi S,Gautier N,Baron V

更新日期：2001-11-02 00:00:00

abstract：:We have cloned a novel nuclear gene for a ribosomal protein of rice and Arabidopsis that is like the bacterial ribosomal protein S9. To determine the subcellular localization of the gene product, we fused the N-terminal region and green fluorescent protein and expressed it transiently in rice seedlings. Localized fluo...

journal_title：FEBS letters

authors： Arimura S,Takusagawa S,Hatano S,Nakazono M,Hirai A,Tsutsumi N

更新日期：1999-05-07 00:00:00

abstract：:We have investigated the effect of botulinum neurotoxin (BoNT) C1 light chain (LC) on insulin exocytosis from the clonal beta-cell line HIT-T15. In streptolysin-O permeabilized cells, the beta-cell impermeant BoNT C1 cleaved mainly syntaxin 1 and inhibited Ca2+ as well as GTPgammaS induced exocytosis. To study the eff...

journal_title：FEBS letters

authors： Land J,Zhang H,Vaidyanathan VV,Sadoul K,Niemann H,Wollheim CB

更新日期：1997-12-08 00:00:00

abstract：:Rats bearing the Yoshida AH-130 ascites hepatoma showed an increased expression of both uncoupling protein-2 (UCP2) (194%) and UCP3 (189%) mRNA levels in skeletal muscle 7 days after tumour inoculation. Interestingly, an even greater increase was observed in mRNA for both UCP2 (278%) and UCP3 (797%) in the pair-fed an...

journal_title：FEBS letters

authors： Sanchís D,Busquets S,Alvarez B,Ricquier D,López-Soriano FJ,Argilés JM

更新日期：1998-10-09 00:00:00

abstract：:In addition to their g = 1.94 EPR signal, nitrogenase Fe-proteins from Azotobacter vinelandii, Azotobacter chroococcum and Klebsiella pneumoniae exhibit a weak EPR signal with g approximately equal to 5. Temperature dependence of the signal was consistent with an S = 3/2 system with negative zero-field splitting, D = ...

journal_title：FEBS letters

authors： Hagen WR,Eady RR,Dunham WR,Haaker H

更新日期：1985-09-23 00:00:00

abstract：:The amino acid sequences of the polypeptide chains of the acetylcholine receptor have recently been published. From the hydrophilicity profiles, it has been proposed that residues 161-166 of the alpha-chain might be an important antigenic site. We have synthesised a peptide containing this sequence and raised antisera...

journal_title：FEBS letters

authors： Juillerat MA,Barkas T,Tzartos SJ

更新日期：1984-03-12 00:00:00

abstract：:The importance of the individual amino acid residues of AIP (KKALRRQEAVDAL), a highly specific inhibitor of calmodulin-dependent protein kinase II (CaMKII), was studied. Replacement of Arg6, Gln7, or Ala9 by other amino acid residues produced a marked increase in the IC50 value. Leu4 and Val10 were also sensitive to r...

journal_title：FEBS letters

authors： Ishida A,Shigeri Y,Tatsu Y,Uegaki K,Kameshita I,Okuno S,Kitani T,Yumoto N,Fujisawa H

更新日期：1998-05-01 00:00:00

abstract：:A catalytic sequence motif PDX10-30(E/D)XK is found in many restriction enzymes. On the basis of sequence similarities and mapping of the conserved residues to the crystal structure of NgoMIV we suggest that residues D160, K182, R186, R188 and E195 contribute to the catalytic/DNA binding site of the Ecl18kI restrictio...

journal_title：FEBS letters

authors： Tamulaitis G,Solonin AS,Siksnys V

更新日期：2002-05-08 00:00:00

abstract：:We report for the first time the identification of 25 microRNAs from tissues originating from chicken embryo and adult chicken. Most of the cloned microRNAs are expressed in both adult chickens and chicken embryos. Fourteen were identified without any prior prediction. One microRNA, miR-757, is thought to be chicken-s...

journal_title：FEBS letters

authors： Xu H,Wang X,Du Z,Li N

更新日期：2006-06-26 00:00:00

abstract：:A novel peptide from Centruroides noxius Hoffmann scorpion venom was isolated and sequenced. The 37 amino acid peptide belongs to the charybdotoxin sub-family (alphaKTx1) and was numbered member 11. alphaKTx1.11 has 75% sequence identity with iberiotoxin and 54% with charybdotoxin. alphaKTx1.11 revealed specificity fo...

journal_title：FEBS letters

authors： Garcia-Valdes J,Zamudio FZ,Toro L,Possani LD

更新日期：2001-09-21 00:00:00

abstract：:We describe the creation of cell adhesion mediated by cell surface engineering. The Flt3-ligand was fused to a membrane anchor made of the diphtheria toxin translocation domain. The fusion protein was attached to the surface of a cell by an acid pulse. Contact with another cell expressing the receptor Flt3 lead to its...

journal_title：FEBS letters

authors： Perier A,Gourier C,Pichard S,Husson J,Lajeunesse E,Babon A,Menez A,Gillet D

更新日期：2007-11-27 00:00:00

abstract：:We have identified in rat vascular smooth muscle cells (SMCs) the simultaneous expression of two TGF-beta type I receptor (ALK-5) cDNAs, occurring as a consequence of alternate usage of AG splice acceptor motifs separated by 12 nucleotides located at an intron-exon junction. When translated the resultant full length p...

journal_title：FEBS letters

authors： Agrotis A,Condron M,Bobik A

更新日期：2000-02-04 00:00:00

abstract：:Previously determined protein structures have been analysed, in order to find folding motifs similar to that proposed by NMR spectroscopy, for the N-terminal, 14 kDa fragment of the Ada protein. The analyses reveal only limited similarities with the NMR-derived structural data and strongly suggest that this region of ...

journal_title：FEBS letters

authors： Swindells MB

更新日期：1993-06-01 00:00:00

abstract：:The Fas/Fas ligand interaction plays a crucial role in various liver diseases, and administration of agonistic anti-Fas antibody to mice causes massive hepatic apoptosis and fulminant hepatic failure. Several growth factors have recently been found to function in preventing apoptosis. In this study, we demonstrated th...

journal_title：FEBS letters

authors： Kanda D,Takagi H,Toyoda M,Horiguchi N,Nakajima H,Otsuka T,Mori M

更新日期：2002-05-22 00:00:00

abstract：:The delta-endotoxin produced by Bacillus thuringiensis ssp. entomocidus induced the release of encapsulated [14C]sucrose from reverse-phase vesicles composed of phosphatidylcholine and cholesterol. No such release was detected when the phospholipid component of the vesicles was either phosphatidylethanolamine, phospha...

journal_title：FEBS letters

authors： Yunovitz H,Yawetz A

更新日期：1988-03-28 00:00:00

abstract：:Neuronal activity is often associated with changes in gene expression. By a two-dimensional cDNA-display system, restriction landmark cDNA scanning, we identified a novel gene whose expression in the hippocampus was up-regulated by kainate stimulation. The mRNA expression was detected only in brain and up-regulated by...

journal_title：FEBS letters

authors： Nakayama T,Yaoi T,Yasui M,Kuwajima G

更新日期：1998-05-22 00:00:00

abstract：:In this work, we investigated electron transport around the photosynthetic pigment-protein complex of Photosystem I (PS I) mediated by external high-potential electron carrier 2,3-dichloro-1,4-naphtoquinone (Cl2 NQ) and ascorbate. It has been demonstrated that the oxidized species of Cl2 NQ and ascorbate serve as inte...

journal_title：FEBS letters

authors： Petrova AA,Trubitsin BV,Boskhomdzhieva BK,Semenov AY,Tikhonov AN

更新日期：2018-07-01 00:00:00

abstract：:We used Xenopus oocytes co-expressing thyrotropin-releasing hormone (TRH) receptors and human ether-a-go-go-related gene (HERG) K+ channel variants carrying different amino-terminal modifications to check the relevance of the proximal domain for hormonal regulation of the channel. Deletion of the whole proximal domain...

journal_title：FEBS letters

authors： Gómez-Varela D,Barros F,Viloria CG,Giráldez T,Manso DG,Dupuy SG,Miranda P,de la Peña P

更新日期：2003-01-30 00:00:00

abstract：:The glycogen branching enzyme from Vibrio vulnificus (VvGBE) transfers short side chains (DP 3-5) significantly greater than any other bacterial glycogen branching enzyme (GBE). To elucidate the role of the N-domain of VvGBE in the unique branching pattern, domain-truncated (N1 and N) and N1-domain-swapped (with VvGBE...

journal_title：FEBS letters

authors： Jo HJ,Park S,Jeong HG,Kim JW,Park JT

更新日期：2015-04-28 00:00:00

abstract：:Annexin I (AnxI) is a calcium-dependent membrane binding protein which has been implicated in various physiological activities. The region of the chicken anxI cDNA encoding the first 130 amino terminal residues was cloned by reverse transcription PCR in order to determine the relationship of its variable amino-termina...

journal_title：FEBS letters

authors： Sidis Y,Horseman ND

更新日期：1993-08-30 00:00:00

abstract：:With the aging of population, neurological disorders, and especially disorders involving defects in protein conformation (also known as proteopathies) pose a serious socio-economic problem. So far there is no effective treatment for most proteopathies, including Parkinson's disease (PD). The mechanism underlying PD pa...

journal_title：FEBS letters

authors： Oláh J,Ovádi J

更新日期：2019-07-01 00:00:00

abstract：:Multiple protein kinase C (PKC) theta species, identified in an erythroleukaemia cell line, have been characterised in terms of their molecular properties and intracellular distribution. PKCthetas localised in the detergent-soluble cell fraction have an Mr of 76 kDa (theta-76) and contain Thr538 or pThr538 in the kina...

journal_title：FEBS letters

authors： Sparatore B,Passalacqua M,Pedrazzi M,Ledda S,Patrone M,Gaggero D,Pontremoli S,Melloni E

更新日期：2003-11-06 00:00:00

abstract：:The effect of platelet-activating factor (PAF) on polyphosphoinositide metabolism and 45Ca2+ efflux was examined in a vascular smooth muscle cell line (A7r5). PAF stimulated a rapid but transient production of inositol trisphosphate and inositol bisphosphate which, in the presence of lithium, resulted in an accumulati...

journal_title：FEBS letters

authors： Doyle VM,Creba JA,Rüegg UT

更新日期：1986-03-03 00:00:00