Abstract:
:Multiple protein kinase C (PKC) theta species, identified in an erythroleukaemia cell line, have been characterised in terms of their molecular properties and intracellular distribution. PKCthetas localised in the detergent-soluble cell fraction have an Mr of 76 kDa (theta-76) and contain Thr538 or pThr538 in the kinase activation loop. In contrast, PKCthetas localised in the Golgi complex have an Mr of 85 kDa (theta-85) and, although unphosphorylated at Thr538, are catalytically active. Strikingly, only theta-76 species which are unphosphorylated at Thr538 can undergo autocatalytic conversion to theta-85. Moreover, a Thr538-->Ala PKCtheta mutant is constitutively localised in the Golgi complex, confirming that changes in the phosphorylation state of this residue play a pivotal role in the overall control of catalytic properties and localisation of this kinase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sparatore B,Passalacqua M,Pedrazzi M,Ledda S,Patrone M,Gaggero D,Pontremoli S,Melloni Edoi
10.1016/s0014-5793(03)01073-1keywords:
subject
Has Abstractpub_date
2003-11-06 00:00:00pages
35-40issue
1-2eissn
0014-5793issn
1873-3468pii
S0014579303010731journal_volume
554pub_type
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