Abstract:
:CD133 is a widely used cell surface marker of cancer stem cells that plays an important role in tumor initiation and metastasis. Increasing evidence shows that CD133 is secreted to the extracellular space. However, the underlying mechanisms of CD133 secretion remain largely unknown. In this study, we report that secreted CD133 has a complex-type N-glycosylation and is modified by beta1,6GlcNAc N-glycan. We found that inhibition of CD133 complex-type N-glycosylation by swainsonine does not affect the membrane localization of CD133, but significantly reduces CD133 secretion and promotes its accumulation in early endosomes. Moreover, swainsonine reduces CD133 secretion by reducing its mono-ubiquitination and inhibiting the interaction between CD133 and Tsg101. These findings reveal a new mechanism of glycosylation-dependent secretion of CD133.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Li Y,Shi D,Yang F,Chen X,Xing Y,Liang Z,Zhuang J,Liu W,Gong Y,Jiang J,Wei Ydoi
10.1002/1873-3468.13358subject
Has Abstractpub_date
2019-04-01 00:00:00pages
719-731issue
7eissn
0014-5793issn
1873-3468journal_volume
593pub_type
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