The functional role of the beta-subunit in the maturation and intracellular transport of Na,K-ATPase.

abstract：:The effects of intracellular purine nucleotides on tolbutamide-induced block of ATP-dependent K+ channels from mouse pancreatic B-cells were studied using the patch-clamp technique. When applied to the inside of excised patches, tolbutamide alone blocked channel activity half-maximally at 55 microM and the concentrati...

journal_title：FEBS letters

authors： Zünkler BJ,Lins S,Ohno-Shosaku T,Trube G,Panten U

更新日期：1988-11-07 00:00:00

abstract：:To characterize energy metabolism in rat brown adipose tissue (BAT), we carried out differential screening of a cDNA library of BAT with a cDNA probe of white adipose tissue (WAT) and isolated one cDNA clone. It contained a single open reading frame of 2,316 bases which encodes a protein of 88.2 kDa. The predicted ami...

journal_title：FEBS letters

authors： Yamazaki N,Shinohara Y,Shima A,Terada H

更新日期：1995-04-17 00:00:00

abstract：:The present study shows a direct impairing action of a cytotoxin-producing Helicobacter pylori strain on the Na+,K(+)-ATPase (evaluated as K(+)-dependent phosphatase activity) of human gastric epithelial cells in culture. The toxin itself is likely involved in this action which may also account for the cell edema foun...

journal_title：FEBS letters

authors： Ricci V,Sommi P,Cova E,Fiocca R,Romano M,Ivey KJ,Solcia E,Ventura U

更新日期：1993-11-15 00:00:00

abstract：:The prohormone convertase PC2 is one of the major subtilisin/kexin-like enzymes responsible for the formation of small bioactive peptides in neural and endocrine cells. This convertase is unique among the members of the subtilisin/kexin-like mammalian serine proteinase family in that it undergoes zymogen processing of...

journal_title：FEBS letters

authors： Benjannet S,Mamarbachi AM,Hamelin J,Savaria D,Munzer JS,Chrétien M,Seidah NG

更新日期：1998-05-22 00:00:00

abstract：:Trypsin treatment of HeLa cells results in a limited proteolysis of the coxsackievirus and adenovirus receptor (CAR) after which the cleaved CAR remains cell-associated and tryptic peptides remain associated through disulfide bonds. Trypsin-treated HeLa cells remain susceptible to infection with coxsackievirus B and p...

journal_title：FEBS letters

authors： Carson SD

更新日期：2000-11-03 00:00:00

abstract：:The protein kinase C activator, phorbol-12-myristate-13-acetate (PMA), augments the cyclic AMP accumulation induced by forskolin in pheochromocytoma (PC 12) cells with an EC50 value of 14 nM, while having no effect on basal values. At a concentration of 100 nM PMA markedly augmented the magnitude of the forskolin resp...

journal_title：FEBS letters

authors： Hollingsworth EB,Ukena D,Daly JW

更新日期：1986-02-03 00:00:00

abstract：:The pl6INK4a tumor suppressor negatively regulates progression through the G1 phase of the mammalian cell cycle. To mimic the downmodulation of p16INK4a commonly seen in cancer, we designed and characterized a hammerhead ribozyme against exon E1alpha of the murine pl6INK4a transcript. Stable expression of the ribozyme...

journal_title：FEBS letters

authors： Nylandsted J,Rohde M,Bartek J,Strauss M

更新日期：1998-09-25 00:00:00

abstract：:A reversible two-step (native state<-->denatured state) folding mechanism based on equilibrium and stopped flow experiments is proposed for urea denaturation of the lipoyl-bearing domain (hbLBD) of human mitochondrial branched chain alpha-ketoacid dehydrogenase (BCKD) complex. The results from this circular dichroism ...

journal_title：FEBS letters

authors： Naik MT,Chang YC,Huang TH

更新日期：2002-10-23 00:00:00

abstract：:In order to study the transactivational property of the X gene in the methylotropic yeast Pichia pastoris, a Rous sarcoma virus-chloramphenicol acetyltransferase (RSV-CAT) cassette was co-transformed and integrated into the host yeast strain as a reporter which showed an overwhelming CAT activity. Immunoprecipitation ...

journal_title：FEBS letters

authors： Lal SK,Kumar V

更新日期：1999-07-30 00:00:00

abstract：:To determine the regulatory mechanism for human tyrosine hydroxylase, we examined modulations of the activity of the enzyme from human pheochromocytoma by cyclic AMP-dependent protein kinase, calmodulin-dependent protein kinase II and polyanion. The most remarkable activation was observed when the enzyme was assayed a...

journal_title：FEBS letters

authors： Okuno S,Kanayama Y,Fujisawa H

更新日期：1989-08-14 00:00:00

abstract：:The bacterial twin-arginine translocation (Tat) pathway is distinct from the Sec system by its remarkable capacity to export folded enzymes. To address the question whether the two systems are capable of translocating homologous enzymes catalyzing the same reaction, we cloned the tap gene encoding Thermus thermophilus...

journal_title：FEBS letters

authors： Angelini S,Moreno R,Gouffi K,Santini C,Yamagishi A,Berenguer J,Wu L

更新日期：2001-10-05 00:00:00

abstract：:Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydroge...

journal_title：FEBS letters

authors： Shin EY,Min DS,Shin JC,Shin KS,Hyun MS,Ha KS,Kim HS,Ahn HY,Kim EG

更新日期：2001-11-16 00:00:00

abstract：:Recently, we cloned several fluorescent proteins of different colors homologous to Aequorea victoria green fluorescent protein, which have great biotechnological potential as in vivo markers of gene expression. However, later investigations revealed severe drawbacks in the use of novel fluorescent proteins (FPs), in p...

journal_title：FEBS letters

authors： Yanushevich YG,Staroverov DB,Savitsky AP,Fradkov AF,Gurskaya NG,Bulina ME,Lukyanov KA,Lukyanov SA

更新日期：2002-01-30 00:00:00

abstract：:In this study, we demonstrated that Ent-11alpha-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F) had stronger cytotoxicity against MKN-45, a gastric cancer cell line bearing wild-type p53 than MKN-28, another gastric cancer cell line containing missense mutation in p53. The rapid increase of ROS level was involved in the me...

journal_title：FEBS letters

authors： Liu Z,Ng EK,Liang NC,Deng YF,Leung BC,Chen GG

更新日期：2005-02-28 00:00:00

abstract：:Cyclic ADP-ribose (cADPR), an endogenous NAD+ metabolite in many mammalian and invertebrate tissues, is a potent mediator of calcium mobilization in sea urchin eggs. Our results show that cADPR also stimulates calcium release from rat brain microsomes, marked release occurring over the concentration range 10-250 nM. T...

journal_title：FEBS letters

authors： White AM,Watson SP,Galione A

更新日期：1993-03-08 00:00:00

abstract：:Capsaicin, a major ingredient of hot pepper, is considered to exhibit anti-inflammatory properties. Our previous study demonstrated that capsaicin inhibited the production of pro-inflammatory mediators through NF-kappaB inactivation in LPS-stimulated macrophages. In order to further clarify the mechanism underlying th...

journal_title：FEBS letters

authors： Park JY,Kawada T,Han IS,Kim BS,Goto T,Takahashi N,Fushiki T,Kurata T,Yu R

更新日期：2004-08-13 00:00:00

abstract：:The vasopressin gene is highly transcribed in magnocellular neurons of the supraoptic (SON) and paraventricular nucleus (PVN) in the rat hypothalamus. In order to identify cis-acting elements involved in the expression of the vasopressin gene, approximately 1 kb upstream of the transcription start site has been sequen...

journal_title：FEBS letters

authors： Mohr E,Richter D

更新日期：1990-01-29 00:00:00

abstract：:A full-length cDNA clone encoding steroid 11 beta-hydroxylase (P-45011 beta) has been isolated from a cDNA library derived from human adrenal tumor. The insert of the clone contains an open reading frame encoding a protein of 503 amino acid residues together with a 4 bp 5'-untranslated region and a 576 bp 3'-untransla...

journal_title：FEBS letters

authors： Kawamoto T,Mitsuuchi Y,Toda K,Miyahara K,Yokoyama Y,Nakao K,Hosoda K,Yamamoto Y,Imura H,Shizuta Y

更新日期：1990-09-03 00:00:00

abstract：:tRNAs play a central role in protein translation, acting as the carrier of amino acids. By cloning microRNAs, we unexpectedly obtained some tRNA fragments generated by tRNA cleavage in the anticodon loop. These tRNA fragments are present in many cell lines and different mouse tissues. In addition, various stress condi...

journal_title：FEBS letters

authors： Fu H,Feng J,Liu Q,Sun F,Tie Y,Zhu J,Xing R,Sun Z,Zheng X

更新日期：2009-01-22 00:00:00

abstract：:The roles of mild uncoupling caused by free fatty acids (mediated by plant uncoupling mitochondrial protein (PUMP) and ATP/ADP carrier (AAC)) and non-coupled respiration (alternative oxidase (AO)) on H(2)O(2) formation by plant mitochondria were examined. Both laurate and oleate prevent H(2)O(2) formation dependent on...

journal_title：FEBS letters

authors： Casolo V,Braidot E,Chiandussi E,Macrì F,Vianello A

更新日期：2000-05-26 00:00:00

abstract：:A bovine anterior pituitary polypeptide that stimulates the replication of rat and human adipocyte precursors has been purified. Its Mr is 44 000-53 000 and its isoelectric point is 9.8-10.3. While pituitary basic fibroblast growth factor is equally mitogenic on adipocyte precursors and skin fibroblasts, the polypepti...

journal_title：FEBS letters

authors： Lau DC,Roncari DA,Yip DK,Kindler S,Nilsen SG

更新日期：1983-03-21 00:00:00

abstract：:Uptake of taurine in human placental brush-border membrane vesicles was greatly stimulated in the presence of an inwardly-directed Na+ + Cl- -gradient and uphill transport of taurine could be demonstrated under these conditions. Na+ as well as Cl- were obligatory for this uptake and both ion gradients could energize t...

journal_title：FEBS letters

authors： Miyamoto Y,Balkovetz DF,Leibach FH,Mahesh VB,Ganapathy V

更新日期：1988-04-11 00:00:00

abstract：:The verprolin family of proteins, WIP, CR16 and WIRE/WICH, has emerged as critical regulators of cytoskeletal organisation in vertebrate cells. The founding father of the family, verprolin, was originally identified in budding yeast and later shown to be needed for actin polymerisation during polarised growth and duri...

journal_title：FEBS letters

authors： Aspenström P

更新日期：2005-10-10 00:00:00

abstract：:There are numerous examples of how fundamental research has been required to understand and treat human disease. This article focuses on three human diseases of lipid metabolism in which advancements in understanding and treatment would not have been possible without basic research. Fabry disease is an inherited metab...

journal_title：FEBS letters

authors： Vance DE

更新日期：2006-10-09 00:00:00

abstract：:Two lipophilic derivatives of caffeic acid which inhibit lipoxygenase, caffeic acid phenethyl ester (CAPE) and N,N'-dicyclohexyl-O-(3,4-dihydroxycinnamoyl)-isourea (DCHCU), reduced the proliferative response of murine splenocytes to concanavalin A in vitro. Both CAPE and DCHCU induced apoptosis in murine thymocyte cul...

journal_title：FEBS letters

authors： Mirzoeva OK,Yaqoob P,Knox KA,Calder PC

更新日期：1996-11-04 00:00:00

abstract：:A number of recent observations have suggested that the endocytic and biosynthetic pathways may share fundamentally similar transport mechanisms at the molecular level. Some of the more striking of these suggestions have come from a comparison of the effects of the macrocyclic lactone brefeldin A (BFA) on endosomes an...

journal_title：FEBS letters

authors： Hunziker W,Whitney JA,Mellman I

更新日期：1992-07-27 00:00:00

abstract：:Protamines are polycationic proteins that are widely used for neutralisation of the anticoagulant action of heparin. However, several reports have shown adverse, mast cell-dependent reactions to protamine. The exact mechanism by which protamine causes these adverse effects is not clear. In the present study, the possi...

journal_title：FEBS letters

authors： Pejler G

更新日期：1996-04-01 00:00:00

abstract：:Structural analogs of adenosine 5'-acylphosphates, which are intermediates of the reaction catalyzed by acyl-CoA synthetases, were synthesized by condensing primary alcohols with AMP to examine the inhibitory effects on the lignoceroyl-CoA and palmitoyl-CoA synthetase activities. Hexadecyl, octadecyl, eicosyl, docosyl...

journal_title：FEBS letters

authors： Shiraishi T,Tezuka K,Uda Y

更新日期：1994-10-03 00:00:00

abstract：:Bcl-xL, a close homolog of Bcl2, is an important regulator of apoptosis and is overexpressed in human cancer. Phosphorylation of Bcl-xL can be induced by microtubule-damaging drugs such as taxol or 2-methoxyestradiol (2-ME). By site-directed mutagenesis studies, we have identified that serine 62 is the necessary site ...

journal_title：FEBS letters

authors： Basu A,Haldar S

更新日期：2003-03-13 00:00:00

abstract：:The human gene frataxin and its yeast homolog YFH1 affect mitochondrial function. Deficits in frataxin result in Friedreich ataxia, while deletion of YFH1 results in respiratory incompetence. We determined that as long as respiratory incompetent yeast express Yfh1p they do not accumulate excessive mitochondrial iron. ...

journal_title：FEBS letters

authors： Chen OS,Kaplan J

更新日期：2001-11-30 00:00:00