Escherichia coli PII protein: purification, crystallization and oligomeric structure.

abstract：:Besides its canonical function of catalyzing the formation of telomeric repeats, many groups have recently reported non-canonical functions of hTERT in particular, and telomerase in general. Regulating transcription is the central basis of non-canonical functions of telomerase. However, unlike reverse transcriptase ac...

journal_title：FEBS letters

authors： Akıncılar SC,Low KC,Liu CY,Yan TD,Oji A,Ikawa M,Li S,Tergaonkar V

更新日期：2015-04-13 00:00:00

abstract：:Although neurokinin A (NKA), a tachykinin peptide with sequence homology to substance P (SP), is a weak competitor of radiolabeled SP binding to the NK-1 receptor (NK-1R), more recent direct binding studies using radiolabeled NKA have demonstrated an unexpected high-affinity interaction with this receptor. To document...

journal_title：FEBS letters

authors： Bremer AA,Leeman SE,Boyd ND

更新日期：2000-12-01 00:00:00

abstract：:Analysis of nascent gamma B-crystallin peptides accumulating during in vitro translation in a rabbit reticulocyte lysate cell-free system was carried out. As a consequence of the irregular distribution of rare codons along the polypeptide chain of gamma B-crystallin, translation of the two-domain protein is a non-unif...

journal_title：FEBS letters

authors： Komar AA,Jaenicke R

更新日期：1995-12-04 00:00:00

abstract：:Comparative allelotyping of the short arm of human chromosome 3 (3p) in four types of epithelial carcinomas was performed using an identical set of polymorphic markers. In total, 117 samples of non-papillary renal cell carcinoma (RCC), non-small cell lung carcinoma (NSCLC), carcinoma of uterine cervix (CC), and breast...

journal_title：FEBS letters

authors： Braga E,Pugacheva E,Bazov I,Ermilova V,Kazubskaya T,Mazurenko N,Kisseljov F,Liu J,Garkavtseva R,Zabarovsky E,Kisselev L

更新日期：1999-07-09 00:00:00

abstract：:Polyunsaturated N-acylethanolamines (NAEs), including anandamide (20:4n-6 NAE), elicit a variety of biological effects through cannabinoid receptors, whereas saturated and monounsaturated NAEs are inactive. Arachidonic acid mobilization induced by treatment of intact mouse peritoneal macrophages with Ca2+ ionophore A2...

journal_title：FEBS letters

authors： Kuwae T,Shiota Y,Schmid PC,Krebsbach R,Schmid HH

更新日期：1999-10-01 00:00:00

abstract：:The complementary-addressed modification of DNA and proteins in chromatin using photoreactive derivatives of pd(AC)6 has been studied. These oligonucleotides form complementary complexes with specific DNA sequences and modify both DNA and proteins in the vicinity of these regions, and can be used for investigation of ...

journal_title：FEBS letters

authors： Bozhenok LN,Khazina EB,Chernolovskaya EL,Kobets ND

更新日期：1998-11-27 00:00:00

abstract：:The nervous and immune systems have similar functional characteristics. Both have an intricate network of synaptic connections and an exquisite communication system that enable intercellular signal transduction. Although semaphorins were originally identified as guidance cues in neural development, accumulating eviden...

journal_title：FEBS letters

authors： Okuno T,Nakatsuji Y,Kumanogoh A

更新日期：2011-12-01 00:00:00

abstract：:With the ultimate aim of developing an effective antiviral strategy against HIV-1, a mono-DNA enzyme possessing the 10-23 catalytic motif [Santoro and Joyce (1997) Proc. Natl. Acad. Sci. USA 94, 4264-4266] was synthesized against the HIV-1 envelope gene. We tested the in vitro cleavage efficiency of the 178 bp long tr...

journal_title：FEBS letters

authors： Dash BC,Harikrishnan TA,Goila R,Shahi S,Unwalla H,Husain S,Banerjea AC

更新日期：1998-07-24 00:00:00

abstract：:AtCYP20-2 is one of 16 immunophilins in thylakoid lumen. The presence of the isomerase domain in AtCYP20-2, an enrichment of AtCYP20-2 in the stroma membranes and it's co-migration with NAD(P)H dehydrogenase (NDH) in native gels provide evidence that AtCYP20-2 is an auxiliary protein of NDH. When different NDH mutants...

journal_title：FEBS letters

authors： Sirpiö S,Holmström M,Battchikova N,Aro EM

更新日期：2009-07-21 00:00:00

abstract：:A potent inhibitor of a dual-specificity protein phosphatase, VHR (vaccinia H1 related), was isolated during a screening of microbial metabolites. This inhibitor was identified as 4-isoavenaciolide (4-iA), and was determined to irreversibly inhibit VHR phosphatase activity with a 50% inhibitory concentration of 1.2 mi...

journal_title：FEBS letters

authors： Ueda K,Usui T,Nakayama H,Ueki M,Takio K,Ubukata M,Osada H

更新日期：2002-08-14 00:00:00

abstract：:The kinetics of complexing inactivation at identical enzyme and inhibitor concentrations were analyzed and the equations of product generation were derived when the free enzyme concentration is great, larger or smaller than the dissociation constant of inhibitor, K(I). The kinetic constants can be obtained by fitting ...

journal_title：FEBS letters

authors： Wang MH,Zhao KY

更新日期：1997-08-04 00:00:00

abstract：:The influence of the esterification of the 3'(2') hydroxyl group of tRNA on the hydrolysis by venom phosphodiesterase (Crotalus adamanteus) was studied. It was shown that this esterification does not change the rate of hydrolysis. At pH 7-8, no significant differences were observed either when the alpha amino group of...

journal_title：FEBS letters

authors： Yot P,Gueguen P,Chapeville F

更新日期：1968-08-01 00:00:00

abstract：:At least two of the introns in the antithrombin-III (AT-III) gene are located in positions different from those of the other three proteins in this superfamily for which the gene structures are known, namely, ovalbumin, alpha 1-antitrypsin and angiotensinogen. In another part of the 3'-portion of the AT-III gene there...

journal_title：FEBS letters

authors： Jagd S,Vibe-Pedersen K,Magnusson S

更新日期：1985-12-02 00:00:00

abstract：:A monoclonal antibody (mAb) has been produced which reacts with human mitofilin, a mitochondrial inner membrane protein. This mAb immunocaptures its target protein in association with six other proteins, metaxins 1 and 2, SAM50, CHCHD3, CHCHD6 and DnaJC11, respectively. The first three are outer membrane proteins, CHC...

journal_title：FEBS letters

authors： Xie J,Marusich MF,Souda P,Whitelegge J,Capaldi RA

更新日期：2007-07-24 00:00:00

abstract：:We report a new member of the von Willebrand factor A-domain protein superfamily, WARP (for von Willebrand factor A-domain-related protein). The full-length mouse WARP cDNA is 2.3 kb in size and predicts a protein of 415 amino acids which contains a signal sequence, a VA-like domain, two fibronectin type III-like repe...

journal_title：FEBS letters

authors： Fitzgerald J,Tay Ting S,Bateman JF

更新日期：2002-04-24 00:00:00

abstract：:Mox1 and Mox2 homeobox genes have been shown to be critical in axial skeleton and in limb muscle development respectively. Pax1 and Pax3 gene products are also implicated in these processes. Mox and Pax expression patterns are highly overlapping both spatially and temporally during embryonic development. We show here ...

journal_title：FEBS letters

authors： Stamataki D,Kastrinaki M,Mankoo BS,Pachnis V,Karagogeos D

更新日期：2001-06-22 00:00:00

abstract：:Rat calvaria cells grown in culture for one week had properties of osteoblasts: a high content in alkaline phosphatase and a marked cyclic AMP response to parathyroid hormone (PTH). In short-term experiments, insulin-like growth factor I (IGF I) stimulated the incorporation of [14C] glucose into glycogen. When IGF I w...

journal_title：FEBS letters

authors： Schmid C,Steiner T,Froesch ER

更新日期：1984-07-23 00:00:00

abstract：:The informational content of the primary structure of thymopoietin (TP) is investigated using the informational spectrum method (ISM). We show that the sequence of TP shares common information with the sequences of long postsynaptic snake neurotoxins, although no apparent similarity was found among their primary struc...

journal_title：FEBS letters

authors： Skerl V,Pavlović M

更新日期：1988-10-24 00:00:00

abstract：:The sequence of the HIV Nef protein has no significant homology to other proteins in the SwissProt database, and experimental data concerning its function are sparse and contradictory. Using a novel protein sequence comparison method, we find similarities between different Nef sequences and the alpha chain of human MH...

journal_title：FEBS letters

authors： Hobohm U,Sander C

更新日期：1993-11-01 00:00:00

abstract：:We have reported that histone deacetylase (HDAC) inhibitors activate a member of the INK4 family, the p19INK4d gene, causing G1 phase arrest. We report here that HDAC inhibitor, Trichostatin A, activates another member of the INK4 family, the p18INK4c gene, through its promoter in Jurkat cells. Interestingly, the acti...

journal_title：FEBS letters

authors： Yokota T,Matsuzaki Y,Sakai T

更新日期：2004-09-10 00:00:00

abstract：:In this study, we demonstrated that tissue inhibitor of metalloproteinases (TIMP) produced by human bone marrow stromal cell line KM-102 had erythroid-potentiating activity (EPA) which stimulates the proliferation of erythroid progenitor cells. We, then, propose a scheme for the bifunctional role of TIMP/EPA in hemato...

journal_title：FEBS letters

authors： Hayakawa T,Yamashita K,Kishi J,Harigaya K

更新日期：1990-07-30 00:00:00

abstract：:Apolipoprotein E- (ApoE-) rich high-density lipoprotein (HDL), which was prepared from the bound fraction of normolipemic volunteers on heparin-Sepharose and from a hyperalphalipoproteinemic patient, potently inhibited aggregation of human platelets in a dose-dependent fashion. Dimyristoyl phosphatidylcholine liposome...

journal_title：FEBS letters

authors： Higashihara M,Kinoshita M,Teramoto T,Kume S,Kurokawa K

更新日期：1991-04-22 00:00:00

abstract：:The effects of progesterone, dehydroepiandrosterone sulfate and 17beta-estradiol on the synthesis and degradation of hyaluronate were investigated using human uterine cervix fibroblasts. The cells were incubated with [3H]glucosamine in the presence of the hormones and then [3H]hyaluronate was isolated from the medium....

journal_title：FEBS letters

authors： Tanaka K,Nakamura T,Takagaki K,Funahashi M,Saito Y,Endo M

更新日期：1997-02-03 00:00:00

abstract：:The major anthocyanin in A. thaliana is a cyanidin derivative modified by glycosylation as well as by the addition of three acyl moieties: malonyl, p-coumaroyl, and sinapoyl. We have isolated a member of the BAHD acyltransferase family which catalyzes this malonylation reaction by combining a reverse genetics approach...

journal_title：FEBS letters

authors： D'Auria JC,Reichelt M,Luck K,Svatos A,Gershenzon J

更新日期：2007-03-06 00:00:00

abstract：:Mesenchymal stem cells (MSC) transplantation has been shown to decrease fibrosis in the heart; however, whether MSC directly influence the function of cardiac fibroblasts (CFB) remains unknown. MSC-conditioned medium significantly attenuated proliferation of CFB compared with CFB-conditioned medium. MSC-conditioned me...

journal_title：FEBS letters

authors： Ohnishi S,Sumiyoshi H,Kitamura S,Nagaya N

更新日期：2007-08-21 00:00:00

abstract：:Two-photon scanning laser and confocal microscopies were used to image metabolic dynamics of single or cell populations of Saccharomyces cerevisiae strain 28033. Autofluorescence of reduced nicotinamide nucleotides, and mitochondrial membrane potential (DeltaPsim), were simultaneously monitored. Spontaneous, large-sca...

journal_title：FEBS letters

authors： Aon MA,Cortassa S,Lemar KM,Hayes AJ,Lloyd D

更新日期：2007-01-09 00:00:00

abstract：:The somatostatin upstream enhancer (SMS-UE) is a highly complex enhancer element. The distal A-element contains overlapping Pdx1 and Pbx binding sites. However, a point mutation in the A-element that abolishes both Pdxl and Pbx binding does not impair promoter activity. In contrast, a point mutation that selectively e...

journal_title：FEBS letters

authors： Andersen FG,Jensen J,Heller RS,Petersen HV,Larsson LI,Madsen OD,Serup P

更新日期：1999-02-26 00:00:00

abstract：:Phalloidin and fluorescently labeled phalloidin analogs are established reagents to stabilize and mark actin filaments for the investigation of acto-myosin interactions. In the present study, we employed transient and steady-state kinetic measurements as well as in vitro motility assays to show that phalloidin perturb...

journal_title：FEBS letters

authors： Diensthuber RP,Müller M,Heissler SM,Taft MH,Chizhov I,Manstein DJ

更新日期：2011-03-09 00:00:00

abstract：:The secretion mechanisms of cathepsin L from osteoclasts in the process of bone resorption were investigated. The increases in bone pit numbers formed take place by PTH addition in parallel with the increases of cathepsin L and/or L-like proteinase activities in the culture medium of bone cells, and these were suppres...

journal_title：FEBS letters

authors： Tagami K,Kakegawa H,Kamioka H,Sumitani K,Kawata T,Lenarcic B,Turk V,Katunuma N

更新日期：1994-04-11 00:00:00

abstract：:The photocycle of the light-adapted purple membrane was studied with a time-resolved fluorometry apparatus: fluorescence of the sample suspension (greater than 660 nm) was pumped with a 633-nm cw laser and the temporal change induced by a 532-nm pulsed laser was measured with a photon-counting-type transient recorder....

journal_title：FEBS letters

authors： Ohtani H,Itoh H,Shinmura T

更新日期：1992-06-22 00:00:00