Biosynthesis and turnover of anandamide and other N-acylethanolamines in peritoneal macrophages.

Abstract:

:Polyunsaturated N-acylethanolamines (NAEs), including anandamide (20:4n-6 NAE), elicit a variety of biological effects through cannabinoid receptors, whereas saturated and monounsaturated NAEs are inactive. Arachidonic acid mobilization induced by treatment of intact mouse peritoneal macrophages with Ca2+ ionophore A23187 had no effect on the production of NAE or its precursor N-acylphosphatidylethanolamine (N-acyl PE). Addition of exogenous ethanolamine resulted in enhanced NAE synthesis by its N-acylation with endogenous fatty acids, but this pathway was not selective for arachidonic acid. Incorporation of (18)O from H2 (18)O-containing media into the amide carbonyls of both NAE and N-acyl PE demonstrated a rapid, constitutive turnover of both lipids.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Kuwae T,Shiota Y,Schmid PC,Krebsbach R,Schmid HH

doi

10.1016/s0014-5793(99)01226-0

keywords:

subject

Has Abstract

pub_date

1999-10-01 00:00:00

pages

123-7

issue

1

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(99)01226-0

journal_volume

459

pub_type

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