Insulin-like growth factor I supports differentiation of cultured osteoblast-like cells.


:Rat calvaria cells grown in culture for one week had properties of osteoblasts: a high content in alkaline phosphatase and a marked cyclic AMP response to parathyroid hormone (PTH). In short-term experiments, insulin-like growth factor I (IGF I) stimulated the incorporation of [14C] glucose into glycogen. When IGF I was present in the medium during 6 days the cell number increased slightly and there was a substantial, disproportionate rise in alkaline phosphatase activity of the cultures. Thus, IGF I stimulates growth, and in addition, and in contrast to other growth factors, mainly enhances differentiation of osteoblasts.




FEBS letters


Schmid C,Steiner T,Froesch ER




Has Abstract


1984-07-23 00:00:00














  • Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strain.

    abstract::The 40 N-terminal amino acids of the 20 kDa antigen A2 from Brucella melitensis were sequenced and showed important similarities with 4 bacterioferrins. A monoclonal antibody raised against this antigen cross-reacted with Escherichia coli bacterioferritin. Hybridization of two sets of degenerate primers with B. melite...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Denoel PA,Zygmunt MS,Weynants V,Tibor A,Lichtfouse B,Briffeuil P,Limet JN,Letesson JJ

    更新日期:1995-03-20 00:00:00

  • Biotin synthase mechanism: on the origin of sulphur.

    abstract::Biotin synthase catalyses the last step of the biosynthesis of biotin in microorganisms and plants. The active protein isolated from Bacillus sphaericus and Escherichia coli contains an iron-sulphur (FeS) cluster. The native enzymes were depleted of their iron and inorganic sulphide and the resulting apoenzymes were c...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bui BT,Florentin D,Fournier F,Ploux O,Méjean A,Marquet A

    更新日期:1998-11-27 00:00:00

  • Ecm10, a novel hsp70 homolog in the mitochondrial matrix of the yeast Saccharomyces cerevisiae.

    abstract::Members of the heat shock protein 70 (Hsp70) family are found in most of the compartments of eukaryotic cells where they play essential roles in protein metabolism. In yeast mitochondria, two Hsp70 proteins are known: Ssc1 and Ssq1. We identified Ecm10 as a third Hsp70 protein in the mitochondrial matrix. Ecm10 shares...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Baumann F,Milisav I,Neupert W,Herrmann JM

    更新日期:2000-12-29 00:00:00

  • Cloning and sequencing of spinach cDNA clones encoding the 20 kDa PS I polypeptide.

    abstract::Apart from the 8 kDa subunit, which is of chloroplast origin, most of the small polypeptides of the PS I reaction center from higher plants are encoded in nuclear genes. We describe here the first nucleotide sequence of a nuclear component of this photosystem, the precursor of the 20 kDa protein. The deduced sequence ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lagoutte B

    更新日期:1988-05-23 00:00:00

  • Isoform-specific interaction of pyruvate kinase with hepatitis C virus NS5B.

    abstract::Hepatitis C virus (HCV) replication and pathogenesis involve both virus-encoded proteins and cellular factors. In our study, we showed that NS5B, the HCV RNA-dependent RNA polymerase, interacted with M2 type pyruvate kinase (M2PK) but not L type pyruvate kinase. We confirmed the interaction by GST pull down, coimmunop...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wu X,Zhou Y,Zhang K,Liu Q,Guo D

    更新日期:2008-06-25 00:00:00

  • Identification of a wheat germ agglutinin-sensitive ATPase in yeast nuclei.

    abstract::We have found that wheat germ agglutinin (WGA), a lectin that specifically binds to N-acetylglucosamine residues inhibits the in vitro transport of plasmid DNA, pJDB219, into yeast nuclei. Histochemical staining of the isolated nuclei with biotinylated WGA and streptavidin-biotinylated peroxidase complex revealed the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tsuchiya E,Hiraga K,Fukui S,Miyakawa T

    更新日期:1989-07-03 00:00:00

  • Calmodulin interacts with the cytoplasmic tails of the parathyroid hormone 1 receptor and a sub-set of class b G-protein coupled receptors.

    abstract::Parathyroid hormone (PTH) binds to its receptor (PTH 1 receptor, PTH1R) and activates multiple pathways. The PTH1R, a class b GPCR, contains consensus calmodulin-binding motifs. The PTH1R cytoplasmic tail interacts with calmodulin in a calcium-dependent manner via the basic 1-5-8-14 motif. Calcium-dependent calmodulin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mahon MJ,Shimada M

    更新日期:2005-01-31 00:00:00

  • Modeling by homology of RNA binding domain in A1 hnRNP protein.

    abstract::Eukaryotic nuclear RNA binding proteins share a common sequence motif thought to be implicated in RNA binding. One of the two domains present in A1 hnRNP protein, has been modelled by homology in order to make a prediction of the main features of the RNA binding site. Acylphosphatase (EC was selected as templ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ghetti A,Bolognesi M,Cobianchi F,Morandi C

    更新日期:1990-12-17 00:00:00

  • Secondary structure and limited proteolysis give experimental evidence that the precursor of pulmonary surfactant protein B contains three saposin-like domains.

    abstract::The 42 kDa precursor of surfactant protein B generates the 79 residue mature SP-B polypeptide, which belongs to the family of saposin-like proteins and has unique functional roles in pulmonary surfactant. From sequence comparisons it has been suggested that proSP-B, in addition to SP-B, contains two saposin-like domai...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zaltash S,Johansson J

    更新日期:1998-02-13 00:00:00

  • Convenient plasmid vectors for construction of chimeric mouse/human antibodies.

    abstract::Chimeric antibodies composed of mouse-derived variable regions and human-derived constant regions have been developed for clinical use. However, construction of chimeric mouse/human genes in expression vectors is time-consuming work. In this study, we developed convenient vectors for construction of chimeric mouse/hum...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kameyama K,Imai K,Itoh T,Taniguchi M,Miura K,Kurosawa Y

    更新日期:1989-02-27 00:00:00

  • EXAFS of the type-1 copper site of rusticyanin.

    abstract::Extended X-ray absorption fine structure (EXAFS) spectra at the Cu K-edge have been recorded of the oxidized and reduced form at pH 3.5 of rusticyanin, the type-1 or 'blue'-copper protein from Thiobacillus ferrooxidans. The EXAFS of oxidized rusticyanin is well simulated with models assuming a ligand set of 2 N(His) a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Holt SD,Piggott B,Ingledew WJ,Feiters MC,Diakun GP

    更新日期:1990-08-20 00:00:00

  • Redox control of iron regulatory protein 2 stability.

    abstract::Iron regulatory protein 2 (IRP2) is a critical switch for cellular and systemic iron homeostasis. In iron-deficient or hypoxic cells, IRP2 binds to mRNAs containing iron responsive elements (IREs) and regulates their expression. Iron promotes proteasomal degradation of IRP2 via the F-box protein FBXL5. Here, we explor...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hausmann A,Lee J,Pantopoulos K

    更新日期:2011-02-18 00:00:00

  • Cross-linking of a synthetic partial-length (1-28) peptide of the Alzheimer beta/A4 amyloid protein by transglutaminase.

    abstract::Cerebral deposits of beta/A4 amyloid protein is a pathologic sign of Alzheimer's disease. A synthetic partial-length (1-28) peptide of this protein contains one glutamine and two lysine residues. Here we show that this peptide can be a substrate of transglutaminase, which catalyzes cross-linking between glutamine and ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ikura K,Takahata K,Sasaki R

    更新日期:1993-07-12 00:00:00

  • 31P-NMR studies of Mycoplasma gallisepticum cells using a continuous perfusion technique.

    abstract::31P-NMR studies of Mycoplasma gallisepticum cells have been carried out using a continuous perfusion technique; these are the first such studies with this organism. Using this technique, glucose metabolism was monitored in the intact organisms, and cell extracts were prepared to identify the intermediates. Under glyco...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Egan W,Barile M,Rottem S

    更新日期:1986-08-18 00:00:00

  • Aminoacyl-coenzyme A synthesis catalyzed by a CoA ligase from Penicillium chrysogenum.

    abstract::Coenzyme A ligases play an important role in metabolism by catalyzing the activation of carboxylic acids. In this study we describe the synthesis of aminoacyl-coenzyme As (CoAs) catalyzed by a CoA ligase from Penicillium chrysogenum. The enzyme accepted medium-chain length fatty acids as the best substrates, but the p...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Koetsier MJ,Jekel PA,Wijma HJ,Bovenberg RA,Janssen DB

    更新日期:2011-03-23 00:00:00

  • Negative and positive effects of CpG-methylation on Xenopus ribosomal gene transcription in vitro.

    abstract::Methylation of cytosine-residues in the sequence CpG affects the expression of many genes and generally correlates with reduced transcription. The ribosomal genes of Xenopus laevis were among the first genes to be studied with respect to their DNA methylation, and a loss of methylation during embryonic development cor...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Labhart P

    更新日期:1994-12-19 00:00:00

  • Swimming speed and chemokinetic response of Rhodobacter sphaeroides investigated by natural manipulation of the membrane potential.

    abstract::The delta psi of R. sphaeroides, grown under high light to reduce the levels of light-harvesting bacteriochlorophyll, was naturally manipulated using light intensity. The relationship between delta psi and the swimming speed of free swimming populations of cells was investigated. After de-energisation by incubation in...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Harrison DM,Packer HL,Armitage JP

    更新日期:1994-07-04 00:00:00

  • Different adhesion inhibiting activities of antisera against plasma membranes of liver and Morris hepatoma 7777.

    abstract::Cell-substratum adhesion of rat hepatocytes was inhibited by antisera raised against plasma membranes of liver (anti-liver antiserum) and Morris hepatoma 7777 (anti-hepatoma antiserum). Similar concentrations of both antisera inhibited adhesion on collagen. Anti-liver antiserum also inhibited the adhesion of hepatocyt...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Neumeier R,Dethlefs U,Reutter W

    更新日期:1984-03-26 00:00:00

  • Prediction of the maximal stability temperature of monomeric globular proteins solely from amino acid sequence.

    abstract::Globular protein thermostability is characterized the cold denaturation, maximal stability (Tms) and heat denaturation temperatures. For mesophilic globular proteins, Tms typically ranges from -25 degrees C to +35 degrees C. We show that the indirect estimate of Tms from calorimetry and the direct estimate from chemic...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ganesh C,Eswar N,Srivastava S,Ramakrishnan C,Varadarajan R

    更新日期:1999-07-02 00:00:00

  • Temporary inhibition of papain by hairpin loop mutants of chicken cystatin. Distorted binding of the loops results in cleavage of the Gly(9)-Ala10 bond.

    abstract::Temporary inhibition of the cysteine proteinases papain and cathepsin L was observed with several hairpin loop mutants of recombinant chicken cystatin at enzyme concentrations above nanomolar. Kinetic modelling of inhibition data, gel electrophoresis and amino acid sequencing revealed that reappearance of papain activ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Machleidt W,Nägler DK,Assfalg-Machleidt I,Stubbs MT,Fritz H,Auerswald EA

    更新日期:1995-03-20 00:00:00

  • Hepatitis B virus X increases immune cell recruitment by induction of chemokine SDF-1.

    abstract::Hepatitis B virus X protein is a major factor in the HBV-induced disease developments. Stromal cell-derived factor-1 is a small cytokine that is strongly chemotactic for lymphocytes. We explored the role of HBx on recruitment of HBV-induced virus-nonspecific immune cells into liver. Immune cell recruitment and SDF-1 e...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cho HK,Kim SY,Seong JK,Cheong J

    更新日期:2014-03-03 00:00:00

  • Human Tudor staphylococcal nuclease (Tudor-SN) protein modulates the kinetics of AGTR1-3'UTR granule formation.

    abstract::Human Tudor staphylococcal nuclease (Tudor-SN) interacts with the G3BP protein and is recruited into stress granules (SGs), the main type of discrete RNA-containing cytoplasmic foci structure that is formed under stress conditions. Here, we further demonstrate that Tudor-SN binds and co-localizes with AGTR1-3'UTR (3'-...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gao X,Shi X,Fu X,Ge L,Zhang Y,Su C,Yang X,Silvennoinen O,Yao Z,He J,Wei M,Yang J

    更新日期:2014-06-13 00:00:00

  • The algorithm of estimation of the Km values for primers of various structure and length in the polymerization reaction catalyzed by Klenow fragment of DNA polymerase I from E. coli.

    abstract::DNA synthesis at primers d(pT)n, d(pA)n, d(pC)n, and d(pG)n in the presence of corresponding complementary templates and at hetero-oligoprimers complementary to M13 phage DNA was investigated. The values of both -log Km and log Vmax increased linearly if homo-oligoprimers contained less than 10 nucleotides. The length...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nevinsky GA,Nemudraya AV,Levina AS,Khomov VV

    更新日期:1989-11-20 00:00:00

  • Calpains are involved in phosphatidylinositol 3',4'-bisphosphate synthesis dependent on the alpha IIb beta 3 integrin engagement in thrombin-stimulated platelets.

    abstract::In thrombin-stimulated platelets alpha IIb beta 3 integrin engagement triggers both phosphatidylinositol 3',4'-bisphosphate synthesis and calpain activation. We checked the possible involvement of calpains in phosphatidylinositol 3-kinase signalling pathway using a cell permeant specific inhibitor of calpains, calpept...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Montsarrat N,Racaud-Sultan C,Mauco G,Plantavid M,Payrastre B,Breton-Douillon M,Chap H

    更新日期:1997-03-03 00:00:00

  • miR-1273g-3p modulates activation and apoptosis of hepatic stellate cells by directly targeting PTEN in HCV-related liver fibrosis.

    abstract::MicroRNA (miRNA) play a pivotal role in the development of liver fibrosis. However, the functions of miRNA in hepatitis C virus (HCV)-related liver fibrosis remain unclear. In this study, we systematically analyzed the microarray data of the serum miRNA in patients with HCV-induced hepatic fibrosis. Among 41 dysregula...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Niu X,Fu N,Du J,Wang R,Wang Y,Zhao S,Du H,Wang B,Zhang Y,Sun D,Nan Y

    更新日期:2016-08-01 00:00:00

  • Nucleotide sequence of the maltotetraohydrolase gene from Pseudomonas saccharophila.

    abstract::The nucleotide sequence of the Pseudomonas saccharophila gene encoding maltotetraohydrolase (G4-forming amylase) has been determined. The coding region for the G4-forming amylase precursor contained 1653 nucleotides. The deduced precursor protein included an N-terminal 21-residue putative signal peptide; the deduced m...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhou JH,Baba T,Takano T,Kobayashi S,Arai Y

    更新日期:1989-09-11 00:00:00

  • Expression of a chimeric, cGMP-sensitive regulatory subunit of the cAMP-dependent protein kinase type I alpha.

    abstract::To study the fluctuations of cGMP in living cells through changes of energy transfer of dissociable fluorescence labeled subunits, we constructed a cGMP-sensitive probe by combining the N-terminus of the type I regulatory subunit of cAMP-dependent protein kinase (PKA) with the cGMP binding sites of cGMP-dependent prot...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wild N,Herberg FW,Hofmann F,Dostmann WR

    更新日期:1995-11-06 00:00:00

  • Peptidyl-prolyl cis-trans isomerase activity as studied by dynamic proton NMR spectroscopy.

    abstract::Recently the identity of the peptidyl-prolyl cis-trans isomerase (PPIase), which accelerates the cis/trans isomerization of prolyl peptide bonds and cyclophilin, the binding protein for the immunosuppressive drug Cyclosporin A (CsA), was discovered. The PPIase catalysis toward the substrate Suc-Ala-Phe-Pro-Phe-pNA has...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hübner D,Drakenberg T,Forsén S,Fischer G

    更新日期:1991-06-17 00:00:00

  • Selective inhibition of divergent seryl-tRNA synthetases by serine analogues.

    abstract::Seryl-tRNA synthetases (SerRSs) fall into two distinct evolutionary groups of enzymes, bacterial and methanogenic. These two types of SerRSs display only minimal sequence similarity, primarily within the class II conserved motifs, and possess distinct modes of tRNA(Ser) recognition. In order to determine whether the t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ahel D,Slade D,Mocibob M,Söll D,Weygand-Durasevic I

    更新日期:2005-08-15 00:00:00

  • Comment: mitochondrial genes and cancer.

    abstract::As a result of an experimental confluence between carcinogenesis and oxidative phosphorylation, we published in 1971 'A Unitary Hypothesis for Carcinogenesis' [(1971) J. Antibiot. Tokyo 24, 405-417]. According to this hypothesis, damaged mitochondria release mitochondrial genetic material which like that from an oncog...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hadler HI

    更新日期:1989-10-09 00:00:00