Abstract:
:The Xenopus laevis oocyte has endogenous sites that bind muscarinic agonists, which have been pharmacologically characterized as M3 and/or M1 receptor subtypes. In order to define the molecular identify of the receptor protein we have analyzed a Xenopus oocyte cDNA library and cloned a 2.9 kb cDNA fragment encoding a muscarinic receptor (xMR). The deduced amino acid sequence reveals a protein of 484 residues with an apparent molecular weight of 54,188 Da. Amino acid comparison with previously cloned mammalian muscarinic receptors showed a 78% identity with the human m4 subtype, presenting at the same time clustered differences within the amino-terminal region and third intracellular loop Genomic Southern analysis displayed the presence of one main gene belonging to this subtype, and the PCR analysis revealed an intronless gene.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Herrera L,Carvallo P,Antonelli M,Olate Jdoi
10.1016/0014-5793(94)00957-0subject
Has Abstractpub_date
1994-09-26 00:00:00pages
175-9issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(94)00957-0journal_volume
352pub_type
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