Degradation of the yeast MAT alpha 2 transcriptional regulator is mediated by the proteasome.

abstract：:When a cell divides prior to completion of DNA replication, serious DNA damage may occur. Thus, in addition to accuracy, the processivity of the replication forks is important. DNA synthesis at replication forks should be completed in time, and forks overcome aberrant structures on the template DNA, including damaged ...

journal_title：FEBS letters

authors： Hizume K,Araki H

更新日期：2019-07-01 00:00:00

abstract：:We amplified, using the polymerase chain reaction and calcitonin receptor (CTR) specific primers, RNA extracted from medullary thyroid carcinoma (MTC) and the derived TT cell line. Both secrete large amounts of calcitonin. Electrophoresis of amplification products revealed, in both cases, an ethidium bromide-stained b...

journal_title：FEBS letters

authors： Frendo JL,Pichaud F,Mourroux RD,Bouizar Z,Segond N,Moukhtar MS,Jullienne A

更新日期：1994-04-04 00:00:00

abstract：:The activity of the Ca-pumping ATPase of cardiac sarcoplasmic reticulum (SR) is controlled by the phosphorylation of the intrinsic regulatory protein phospholamban (PLB), which affects both the apparent Km(Ca) and the Vmax of the transport process. We have investigated the correlation between phosphorylation of PLB an...

journal_title：FEBS letters

authors： Chiesi M,Schwaller R

更新日期：1989-02-13 00:00:00

abstract：:Xenopus oocytes expressing neuronal alpha 1C, alpha 2 and beta 1b calcium channel subunit cDNAs were used in this study. During two-electric voltage clamp recording the oocyte was injected with 10-20 nl of a 100 mM BAPTA solution. Under these conditions, the endogenous Ca-activated Cl current was completely suppressed...

journal_title：FEBS letters

authors： Charnet P,Bourinet E,Dubel SJ,Snutch TP,Nargeot J

更新日期：1994-05-09 00:00:00

abstract：:Ischemic preconditioning (IPrec) improves post-ischemic dysfunctions of the myocardium along with activation of protein kinase C isozymes including PKCdelta. Moreover, expression of cardio-protective determinants can reduce ischemic damages. Because IPrec is limited in aged hearts, we assessed in an experimental model...

journal_title：FEBS letters

authors： Bartling B,Hilgefort C,Friedrich I,Silber RE,Simm A

更新日期：2003-12-18 00:00:00

abstract：:The structure of the Fab fragment of the monoclonal antibody MAK 33 (kappa/IgG1) at pH 2 was characterized. Spectroscopic and kinetic analysis revealed a molten globule-like state, characterized by elements of secondary structure but less defined tertiary contacts than in the native state. However, some aromatic side ...

journal_title：FEBS letters

authors： Lilie H,Buchner J

更新日期：1995-03-27 00:00:00

abstract：:Cripto-1 and OCT4, expressed in stem cells and cancers, play important roles in tumorigenesis. Here, we demonstrate that Cripto-1 expression is regulated by OCT4 in human embryonic carcinoma NCCIT cells. The endogenous expression of Cripto-1 and OCT4 is significantly reduced during differentiation. Cripto-1 expression...

journal_title：FEBS letters

authors： Park SW,Do HJ,Han MH,Choi W,Kim JH

更新日期：2018-01-01 00:00:00

abstract：:The 5.8S and 28S rRNA sequences of the oomycete Phytophthora megasperma were determined in order to study the secondary structure of these molecules and to assess the phylogenetic position of the oomycetes among the eukaryotes. Preliminary results point to an affiliation between the oomycetes, dinoflagellates and cili...

journal_title：FEBS letters

authors： Van der Auwera G,Chapelle S,De Wachter R

更新日期：1994-01-31 00:00:00

abstract：:We have found that the sign of phototaxis, i.e. the direction of phototactic migration either towards or away from the light source, of wild-type Chlamydomonas reinhardtii depends on its photosynthetic activity. This accounts for the frequently reported transient positive phototaxis that precedes a negative phototaxis...

journal_title：FEBS letters

authors： Takahashi T,Watanabe M

更新日期：1993-12-28 00:00:00

abstract：:Nocardia kirovani cell walls possess a lipidic fraction, a lipolysaccharidic fraction and a peptidoglycan. The lipidic part is composed of glycerides, C(16) and C(18) free fatty acids, nocardic acids and of a carotenoid pigment. The lipopolysaccharidic part is composed of an arabinogalactan esterified by nocardic acid...

journal_title：FEBS letters

authors： Guinand M,Vacheron MJ,Michel G

更新日期：1970-01-15 00:00:00

abstract：:In washed anterior pituitary membranes, there is enough GTP to occupy Ns and therefore to obtain activation of adenylate cyclase by vasointestinal peptide. GTP concentrations needed to obtain adenylate cyclase inhibition by dopamine (above 5 X 10- M) stimulate the adenylate cyclase. The dopamine effect is a blockade o...

journal_title：FEBS letters

authors： Bockaert J,Sebben-Perez M

更新日期：1983-09-05 00:00:00

abstract：:Plasmid pGC20 containing the (dGC)9 insert in SmaI recognition site has been used to study the inhibition of cleavage by different restriction endonuclease due to Z-DNA formation in (dCG)10 sequence of the negatively supercoiled plasmid. Data obtained indicate the different sensitivity of restriction endonucleases to ...

journal_title：FEBS letters

authors： Lesnik EA,Bes'chetnikova ZhA,Maslova RN,Varshavsky JaM

更新日期：1991-03-11 00:00:00

abstract：:Chaperone-mediated autophagy (CMA) is a lysosomal pathway that participates in the degradation of cytosolic proteins. CMA is activated by starvation and in response to stressors that result in protein damage. The selectivity intrinsic to CMA allows for removal of damaged proteins without disturbing nearby functional o...

journal_title：FEBS letters

authors： Kon M,Cuervo AM

更新日期：2010-04-02 00:00:00

abstract：:Incubation of human thrombocytes in the presence of [3H]palmitic acid leads to incorporation of this fatty acid into the alpha subunit of Gs as described [Linder et al., Proc. Natl. Acad. Sci. USA 90 (1993) 3675-3679; Degtyarev et al., Biochemistry 32 (1993) 8057-8061] but also into the catalyst of adenylyl cyclase wh...

journal_title：FEBS letters

authors： Mollner S,Beck K,Pfeuffer T

更新日期：1995-09-11 00:00:00

abstract：:Previously, several studies have demonstrated that autophosphorylation of the C-terminal tyrosine residues (Tyr1316 and Tyr1322) affects the signaling properties of the insulin receptor in vivo. To assess the biochemical consequences of the C-terminal phosphorylation in vitro, we have constructed, purified and charact...

journal_title：FEBS letters

authors： Tennagels N,Bergschneider E,Al-Hasani H,Klein HW

更新日期：2000-08-11 00:00:00

abstract：:Protein kinase A (PKA) signaling is essential for growth and virulence of the fungal pathogen Aspergillus fumigatus. Little is known concerning the regulation of this pathway in filamentous fungi. Employing liquid chromatography-tandem mass spectroscopy, we identified novel phosphorylation sites on the regulatory subu...

journal_title：FEBS letters

authors： Shwab EK,Juvvadi PR,Waitt G,Soderblom EJ,Moseley MA,Nicely NI,Steinbach WJ

更新日期：2017-11-01 00:00:00

abstract：:In this work, we investigated electron transport around the photosynthetic pigment-protein complex of Photosystem I (PS I) mediated by external high-potential electron carrier 2,3-dichloro-1,4-naphtoquinone (Cl2 NQ) and ascorbate. It has been demonstrated that the oxidized species of Cl2 NQ and ascorbate serve as inte...

journal_title：FEBS letters

authors： Petrova AA,Trubitsin BV,Boskhomdzhieva BK,Semenov AY,Tikhonov AN

更新日期：2018-07-01 00:00:00

abstract：:Affinity labeling studies have identified several conserved tyrosine residues in the alpha subunit of the nicotinic acetylcholine receptor (alpha Y93, alpha Y190, and alpha Y198) as being in or near the ligand binding site. Mutagenesis studies from several laboratories have shown that substitution of phenylalanine for...

journal_title：FEBS letters

authors： Aylwin ML,White MM

更新日期：1994-07-25 00:00:00

abstract：:Coronin proteins are widely expressed among eukaryotic organisms. Most coronins consist of a WD-repeat domain followed by a C-terminal coiled coil. Dictyostelium discoideum expresses a single short coronin coronin A, which has been implicated in both actin modulation and multicellular differentiation. Whether coronin ...

journal_title：FEBS letters

authors： Fiedler T,Fabrice TN,Studer V,Vinet A,Faltova L,Kammerer RA,Steinmetz MO,Sharpe T,Pieters J

更新日期：2020-04-16 00:00:00

abstract：:The intermediate in the reaction catalyzed by 4-chlorobenzoate dehalogenase from Pseudomonas sp. CBS3 was identified as 4-chlorobenzoyl-CoA. One component of 4-chlorobenzoate dehalogenase worked as a a 4-chlorobenzoyl-CoA ligase catalyzing the formation of 4-chlorobenzoyl-CoA from 4-chlorobenzoate, coenzyme A and ATP....

journal_title：FEBS letters

authors： Löffler F,Müller R

更新日期：1991-09-23 00:00:00

abstract：:Using a phage display peptide library, we characterized the epitope of two monoclonal antibodies reacting with syndecan-1: B-B2 and B-B4. The identified epitopes QDIT, for B-B2, and LPEV, for B-B4, were found to align with residues 36-39 and 90-93 of the mature protein, respectively. In contrast to B-B4, the B-B2 epit...

journal_title：FEBS letters

authors： Dore JM,Morard F,Vita N,Wijdenes J

更新日期：1998-04-10 00:00:00

abstract：:X-linked inhibitor of apoptosis protein (XIAP) is a potent antagonist of caspases, and functions as a ubiquitin-E3 ligase by itself and for caspases. Recently, NEDD8, a ubiquitin-like modifier, has been suggested to be used for modification of caspase-7 mediated by XIAP. However, it is not clear whether caspase-7 is a...

journal_title：FEBS letters

authors： Nagano T,Hashimoto T,Nakashima A,Kikkawa U,Kamada S

更新日期：2012-06-04 00:00:00

abstract：:The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay usi...

journal_title：FEBS letters

authors： Tatsumi K,Tsuji S,Miwa H,Morisaku T,Nuriya M,Orihara M,Kaneko K,Okano H,Yasui M

更新日期：2009-06-18 00:00:00

abstract：:Three homologous aspartate aminotransferases with virtually identical spatial structures and pairwise amino acid sequence identities of > 40% differ markedly with respect to the yield of renaturation upon dilution from 6 M guanidine hydrochloride (mitochondrial < cytosolic < Escherichia coli). The enzymes also respond...

journal_title：FEBS letters

authors： Widmann M,Christen P

更新日期：1995-12-27 00:00:00

abstract：:Extraction and identification of the non-covalently bound heme groups from crude membrane preparations of photoheterotrophically grown Synechocystis sp. PCC 6803 by reversed phase high performance liquid chromatography and optical spectrophotometry led to the detection of heme O in addition to hemes B and A which latt...

journal_title：FEBS letters

authors： Peschek GA,Wastyn M,Fromwald S,Mayer B

更新日期：1995-09-04 00:00:00

abstract：:The eukaryotic transcription factor NF-kappaB is activated by a large variety of stimuli. We have recently shown that ER stress, caused by an aberrant accumulation of membrane proteins within this organelle, also activates NF-kappaB. Here, we show that activation of NF-kappaB by ER stress requires an increase in the i...

journal_title：FEBS letters

authors： Pahl HL,Baeuerle PA

更新日期：1996-08-26 00:00:00

abstract：:Treatment of rat liver microsomes with 2,5-di(tert-butyl)-1,4-benzohydroquinone caused a dose-related inhibition (Ki congruent to 1 microM) of ATP-dependent Ca2+ sequestration. This was paralleled by a similar impairment of the microsomal Ca2+-stimulated ATPase activity. In contrast, the hydroquinose failed to induce ...

journal_title：FEBS letters

authors： Moore GA,McConkey DJ,Kass GE,O'Brien PJ,Orrenius S

更新日期：1987-11-30 00:00:00

abstract：:Streptomyces lividans 1326-9, a bldA+ strain, and its bldA39 mutant derivative J1725 were transformed with a cosmid containing the pur cluster, which determines the puromycin biosynthetic pathway from Streptomyces alboniger. bldA+ transformants produced puromycin in typical amounts, whereas bldA39 transformants did so...

journal_title：FEBS letters

authors： Tercero JA,Espinosa JC,Jiménez A

更新日期：1998-01-16 00:00:00

abstract：:In photosystem II (PSII) the probability that energy absorbed by core antenna chlorophyll (Chl) is transferred to the reaction center (RC) is extremely high. Although close proximity between antenna Chl ensures a high transfer efficiency, relative pigment orientation can fractionally modify it. This level of refinemen...

journal_title：FEBS letters

authors： Vasil'ev S,Shen JR,Kamiya N,Bruce D

更新日期：2004-03-12 00:00:00

abstract：:To better understand the mechanisms involved in the developmental expression of Trypanosoma cruzi antigens we examined the gene structure and transcription properties of the major shed trypomastigote antigen (SAPA). We report in this paper that SAPA is encoded by a small family of at lest 6 genes which differ mainly i...

journal_title：FEBS letters

authors： Affranchino JL,Pollevick GD,Frasch AC

更新日期：1991-03-25 00:00:00