Abstract:
:Protein kinase A (PKA) signaling is essential for growth and virulence of the fungal pathogen Aspergillus fumigatus. Little is known concerning the regulation of this pathway in filamentous fungi. Employing liquid chromatography-tandem mass spectroscopy, we identified novel phosphorylation sites on the regulatory subunit PkaR, distinct from those previously identified in mammals and yeasts, and demonstrated the importance of two phosphorylation clusters for hyphal growth and cell wall-stress response. We also identified key differences in the regulation of PKA subcellular localization in A. fumigatus compared with other species. This is the first analysis of the phosphoregulation of a PKA regulatory subunit in a filamentous fungus and uncovers critical mechanistic differences between PKA regulation in filamentous fungi compared with mammals and yeast species, suggesting divergent targeting opportunities.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Shwab EK,Juvvadi PR,Waitt G,Soderblom EJ,Moseley MA,Nicely NI,Steinbach WJdoi
10.1002/1873-3468.12886subject
Has Abstractpub_date
2017-11-01 00:00:00pages
3730-3744issue
22eissn
0014-5793issn
1873-3468journal_volume
591pub_type
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