Abstract:
:We investigated transactivation by NANOG in regulating growth and differentiation factor 3 (GDF3) expression in NCCIT cells. GDF3 expression was affected by shRNA-mediated downregulation and by exogenous overexpression of NANOG specifically, as well as by retinoic acid-mediated differentiation. GDF3 transcription was activated by NANOG, and the upstream region (-183 to -1) was sufficient to induce minimal transcriptional activity. Moreover, NANOG binds to the GDF3 minimal promoter in vivo and the transcriptional activity is mediated by NANOG transactivation domain. This study provides the first evidence that NANOG is a transcriptional activator of the expression of the oncogenic growth factor GDF3 in embryonic carcinoma cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Park SW,Lim HY,Do HJ,Sung B,Huh SH,Uhm SJ,Song H,Chung HJ,Kim JH,Kim NH,Kim JHdoi
10.1016/j.febslet.2012.08.013subject
Has Abstractpub_date
2012-09-21 00:00:00pages
3529-35issue
19eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00670-9journal_volume
586pub_type
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