Abstract:
:Treatment of U937 cells with dolichyl phosphate led to an increase in the activity of the ICE family protease CPP32, accompanied with cleavage of pre-CPP32 to generate p17. Peptide inhibitors YVAD-cmk and Z-Asp-CH2-DCB (specific to ICE) and DEVD-CHO (specific to CPP32) blocked the dolichyl phosphate-induced apoptosis. The dolichyl phosphate-induced increase of CPP32 activity was inhibited by adenylate cyclase inhibitors, SQ 22536 and 2',5'-dideoxyadenosine. Dolichyl phosphate caused a transient increase of intracellular cAMP concentration. The results suggest that modulation of cAMP synthesis due to the stimulation of adenylate cyclase by dolichyl phosphate plays a critical role in CPP32 activation and apoptosis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Yokoyama Y,Okubo T,Ozawa S,Nagai F,Ushiyama K,Kano I,Shioda M,Kubo H,Takemura M,Namiki H,Yasugi E,Oshima M,Seyama Y,Kano Kdoi
10.1016/s0014-5793(97)00763-1subject
Has Abstractpub_date
1997-07-21 00:00:00pages
153-6issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(97)00763-1journal_volume
412pub_type
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