Abstract:
:The RNA helicase Rok1 plays a role in rRNA processing and in control of cell cycle progression in Saccharomyces cerevisiae. We identified two upstream open reading frames (uORFs) within the ROK1 5' untranslated region, which inhibited Rok1 translation. Mutating uATG to uAAG or generation of a premature stop codon in the uORFs resulted in increased Rok1p levels. Rok1 protein levels oscillated during the cell cycle, declining at G1/S and increasing at G2. The uAAG1 mutation caused a constitutive level of Rok1 proteins throughout the cell cycle, resulting in significant delays in mitotic bud emergence and recovery from pheromone arrest. Our study reveals that the Rok1 protein level is regulated by uORFs, which is critical in cell cycle progression.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Jeon S,Kim Jdoi
10.1016/j.febslet.2010.10.019subject
Has Abstractpub_date
2010-11-19 00:00:00pages
4593-8issue
22eissn
0014-5793issn
1873-3468pii
S0014-5793(10)00823-9journal_volume
584pub_type
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