The crystal structure of the amidohydrolase VinJ shows a unique hydrophobic tunnel for its interaction with polyketide substrates.

abstract：:Astrocytes (AC) induce blood-brain barrier (BBB) properties in brain endothelial cells (EC). As antioxidative activity (AOA) is assumed to be a BBB characteristic, we tested whether AC improve AOA of EC. Monocultivated AC showed higher AOA [manganese superoxide dismutase (SOD), catalase (Cat), glutathione peroxidase (...

journal_title：FEBS letters

authors： Schroeter ML,Mertsch K,Giese H,Müller S,Sporbert A,Hickel B,Blasig IE

更新日期：1999-04-23 00:00:00

abstract：:The glucuronic acid analogue of 1-deoxynojirimycin, 2(S)-carboxy-3(R), 4(R), 5(S)-trihydroxypiperidine, recently isolated from seeds of Baphia racemosa, is a novel specific inhibitor of human liver beta-D-glucuronidase and alpha-L-iduronidase. No other glycosidases are inhibited. The inhibition of beta-D-glucuronidase...

journal_title：FEBS letters

authors： Cenci di Bello I,Dorling P,Fellows L,Winchester B

更新日期：1984-10-15 00:00:00

abstract：:PsbU is one of the extrinsic proteins in red algal Photosystem II (PSII) and functions to optimize the availability of Ca(2+) and Cl(-) cofactors for water oxidation. To determine the functional residue of PsbU, we constructed various PsbU mutants from a red alga Cyanidium caldarium and reconstituted these mutants wit...

journal_title：FEBS letters

authors： Okumura A,Sano M,Suzuki T,Tanaka H,Nagao R,Nakazato K,Iwai M,Adachi H,Shen JR,Enami I

更新日期：2007-11-13 00:00:00

abstract：:Activity of the plant mitochondrial alternative oxidase (AOX) can be regulated by organic acids, notably pyruvate. To date, only two well-conserved cysteine residues have been implicated in this process. We report the functional expression of two AOX isozymes (Sauromatum guttatum Sg-AOX and Arabidopsis thaliana At-AOX...

journal_title：FEBS letters

authors： Crichton PG,Affourtit C,Albury MS,Carré JE,Moore AL

更新日期：2005-01-17 00:00:00

abstract：:Organelles have been studied traditionally as single units, but a novel concept is now emerging: each organelle has distinct functional zones that regulate specific functions. The Golgi apparatus seems to have various zones, including zones for: glycosylphosphatidylinositol-anchored proteins; proteoglycan, mucin and l...

journal_title：FEBS letters

authors： Sasaki K,Yoshida H

更新日期：2019-09-01 00:00:00

abstract：:Spin-trapping techniques and electron spin resonance (ESR) spectroscopy were used to study the relationship between the effect of streptozotocin (STZ) on pancreatic beta-cells and free radical formation by these cells. Results showed that STZ enhanced generation of the DMPO-OH radical adduct, which is a degradation pr...

journal_title：FEBS letters

authors： Nukatsuka M,Sakurai H,Yoshimura Y,Nishida M,Kawada J

更新日期：1988-11-07 00:00:00

abstract：:Uroguanylin, a member of the guanylin peptide family, is a novel peptide regulator for intestinal salt and water transport. A cDNA encoding a precursor for rat uroguanylin was cloned from a rat jejunum cDNA library and sequenced. The precursor was 106 amino acids long and included a 21 residue putative signal peptide ...

journal_title：FEBS letters

authors： Miyazato M,Nakazato M,Matsukura S,Kangawa K,Matsuo H

更新日期：1996-12-02 00:00:00

abstract：:Calpastatin molecule contains four repeated inhibition domains, each having highly conserved internal regions A, B and C. The synthetic oligopeptides of regions A and C had no calpain inhibition activity while region B oligopeptide showed weak inhibition activity. Real-time biomolecular interaction analysis using a BI...

journal_title：FEBS letters

authors： Takano E,Ma H,Yang HQ,Maki M,Hatanaka M

更新日期：1995-03-27 00:00:00

abstract：:Endothelin-1 (ET-1) dose-dependently increased HIF1α expression in pulmonary artery smooth muscle cells (PASMCs). Inhibition of protein synthesis did not affect ET-1-induced HIF1α expression. The maximum effect of ET-1 was similar to that caused by proteasome inhibitor MG132. Further study indicates that ET-1 also dos...

journal_title：FEBS letters

authors： Li M,Liu Y,Jin F,Sun X,Li Z,Liu Y,Fang P,Shi H,Jiang X

更新日期：2012-11-02 00:00:00

abstract：:The A-rich bulge in paired region P5a of the Tetrahymena intron is a structural feature that is conserved in the sub-group Ib self-splicing introns. We have constructed a series of substitution and deletion mutations in this region of the intron. Kinetic analysis has shown that some of the mutants have a reduced maxim...

journal_title：FEBS letters

authors： Pace U,Szostak JW

更新日期：1991-03-11 00:00:00

abstract：:The human gene frataxin and its yeast homolog YFH1 affect mitochondrial function. Deficits in frataxin result in Friedreich ataxia, while deletion of YFH1 results in respiratory incompetence. We determined that as long as respiratory incompetent yeast express Yfh1p they do not accumulate excessive mitochondrial iron. ...

journal_title：FEBS letters

authors： Chen OS,Kaplan J

更新日期：2001-11-30 00:00:00

abstract：:The single cysteinyl residue 26 of spinach calmodulin was labelled with the thiol-specific bimane fluorescence probe. Following application of stoichiometric quantities of Ca2+ or aluminum ions to the protein, temperature-dependent fluorescence changes (anisotropy, lifetime) could be monitored via the label. From thes...

journal_title：FEBS letters

authors： Yuan SX,Haug A

更新日期：1988-07-04 00:00:00

abstract：:MiR-140 is a microRNA specially involved in chondrogenesis and osteoarthritis pathogenesis. However, its transcriptional regulation and target genes in cartilage development are not fully understood. Here we detected that miR-140 was uniquely expressed in chondrocyte and suppressed by Wnt/β-catenin signalling. The miR...

journal_title：FEBS letters

authors： Yang J,Qin S,Yi C,Ma G,Zhu H,Zhou W,Xiong Y,Zhu X,Wang Y,He L,Guo X

更新日期：2011-10-03 00:00:00

abstract：:A cytosolic calmodulin wave has been described in the pre-replicative phase of rat liver regeneration. Here we demonstrate that this calmodulin increase is reduced by injection of cycloheximide (translation inhibitor) and actinomycin D (transcription inhibitor). In addition, we found that calmodulin mRNA levels are in...

journal_title：FEBS letters

authors： Piõl MR,Berchtold MW,Bachs O,Heizmann CW

更新日期：1988-04-25 00:00:00

abstract：:The function of the coenzyme Q (CoQ) pool in the inner mitochondrial membrane is reviewed in view of recent findings suggesting a supramolecular organization of the mitochondrial respiratory complexes. In spite of the structural evidence for preferential aggregations of the inner membrane components, most kinetic evid...

journal_title：FEBS letters

authors： Lenaz G

更新日期：2001-12-07 00:00:00

abstract：:Phosphorylation of histone variant H2AX at serine 139, named gammaH2AX, has been widely used as a sensitive marker for DNA double-strand breaks (DSBs). gammaH2AX is required for the accumulation of many DNA damage response (DDR) proteins at DSBs. Thus it is believed to be the principal signaling protein involved in DD...

journal_title：FEBS letters

authors： Yuan J,Adamski R,Chen J

更新日期：2010-09-10 00:00:00

abstract：:Eucaryotic DNA is punctuated by many A+T-rich segments that we named A+T-rich linkers. Two types of these A+T-rich linkers can be distinguished: (i) isolated A+T-rich linkers, and (ii) A+T-rich linkers crowded in clusters. We have analysed the distribution of A+T-rich linker across the alpha- and beta-globin gene doma...

journal_title：FEBS letters

authors： Moreau J,Scherrer K

更新日期：1987-08-31 00:00:00

abstract：:Cytoplasmic dynein, a microtubule-based motor protein, is responsible for many cellular functions ranging from cargo transport to cell division. The various functions are carried out by a single isoform of cytoplasmic dynein, thus requiring different forms of motor regulation. A possible pathway to regulate motor func...

journal_title：FEBS letters

authors： Behrens VA,Walter WJ,Peters C,Wang T,Brenner B,Geeves MA,Scholz T,Steffen W

更新日期：2019-02-01 00:00:00

abstract：:Cell-substratum adhesion of rat hepatocytes was inhibited by antisera raised against plasma membranes of liver (anti-liver antiserum) and Morris hepatoma 7777 (anti-hepatoma antiserum). Similar concentrations of both antisera inhibited adhesion on collagen. Anti-liver antiserum also inhibited the adhesion of hepatocyt...

journal_title：FEBS letters

authors： Neumeier R,Dethlefs U,Reutter W

更新日期：1984-03-26 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) Rev is a small RNA-binding protein which is essential for viral replication. To investigate the structure of Rev we have mapped the binding sites of a panel of monoclonal antibodies (mAb) by protein footprinting and identified a mAb protecting amino acids within both the N- ...

journal_title：FEBS letters

authors： Jensen TH,Jensen A,Szilvay AM,Kjems J

更新日期：1997-09-01 00:00:00

abstract：:Transfer RNAs (tRNAs) are ancient macromolecules that have evolved under various environmental pressures as adaptors in translation in all forms of life but also towards alternative structures and functions. The present knowledge on both "canonical" and "deviating" signature motifs retrieved from vertical and horizont...

journal_title：FEBS letters

authors： Pütz J,Giegé R,Florentz C

更新日期：2010-01-21 00:00:00

abstract：:Tumor suppressor WW domain-containing oxidoreductase (WWOX) is depleted in various cancer types. Here we report that WWOX is modified by small ubiquitin-like modifier (SUMO) proteins and represses DU145 prostate cancer tumorigenesis in a SUMOylation-dependent manner. Ectopic WWOX was shown to associate with SUMO2/3 or...

journal_title：FEBS letters

authors： Choi HJ,Park JH,Park JH,Lee KB,Oh SM

更新日期：2015-12-21 00:00:00

abstract：:Photosystem I (PSI) complex of Anabaena variabilis ATCC 29413 consists of at least 11 subunits, 9 of which are resolved by high resolution gel electrophoresis. N-terminal amino acid sequences of the four subunits with molecular masses of 6.8, 5.2, 4.8 and 3.5 kDa were determined. Based on the sequence homology, the 3....

journal_title：FEBS letters

authors： Ikeuchi M,Nyhus KJ,Inoue Y,Pakrasi HB

更新日期：1991-08-05 00:00:00

abstract：:The chemokine RANTES is a potent chemoattractant for eosinophils. RANTES is produced by lung epithelial cells during eosinophil-rich inflammatory diseases such as asthma. In this study, we examined the effects of thiazolidinediones (TZD) on RANTES expression in a human lung epithelial cell line, A549. In A549 cells, i...

journal_title：FEBS letters

authors： Momoi A,Murao K,Imachi H,Sayo Y,Nakamura H,Hosokawa H,Sato M,Fujita J,Okada H,Ishida T,Takahara J

更新日期：1999-06-11 00:00:00

abstract：:Streptococcus pneumoniae (pneumococcus) has evolved sophisticated strategies to survive in several niches within the human body either as a harmless commensal or as a serious pathogen causing a variety of diseases. The dynamic interaction between pneumococci and resident host cells during colonization of the upper res...

journal_title：FEBS letters

authors： Kohler S,Voß F,Gómez Mejia A,Brown JS,Hammerschmidt S

更新日期：2016-11-01 00:00:00

abstract：:Over the past several years, the study of a hereditary tumor syndrome, tuberous sclerosis complex (TSC), has shed light on the regulation of cellular proliferation and growth. TSC is an autosomal dominant disorder that is due to inactivating mutations in TSC1 or TSC2 and characterized by benign tumors (hamartomas) inv...

journal_title：FEBS letters

authors： Han JM,Sahin M

更新日期：2011-04-06 00:00:00

abstract：:Rapidly generated high-titer Semliki Forest virus (SFV) vectors can infect numerous mammalian cell lines and primary cell cultures, and result in high levels of transgene expression. SFV-based expression of transmembrane receptors has been characterized by specific ligand-binding activity and functional responses. Ada...

journal_title：FEBS letters

authors： Lundstrom K,Schweitzer C,Rotmann D,Hermann D,Schneider EM,Ehrengruber MU

更新日期：2001-08-31 00:00:00

abstract：:The present study was designed to investigate whether and how the purinergic stimulation of rat ventricular myocytes modulates the cAMP-dependent pathway. Stimulation of cardiomyocytes with ATPgammaS in the presence of the phosphodiesterase inhibitor IBMX increases by 3-fold intracellular cAMP content. In contrast to ...

journal_title：FEBS letters

authors： Pucéat M,Bony C,Jaconi M,Vassort G

更新日期：1998-07-17 00:00:00

abstract：:The gene for the extracellular ribonuclease of B. pumilus KMM62 (RNase Bp) has been cloned and sequenced. The structural gene for this enzyme is similar to those of the extracellular ribonucleases of B. intermedius 7P (binase) and B. amyloliquefaciens H2 (barnase), as are the regulatory regions of binase and RNase Bp....

journal_title：FEBS letters

authors： Znamenskaya LV,Gabdrakhmanova LA,Chernokalskaya EB,Leshchinskaya IB,Hartley RW

更新日期：1995-01-02 00:00:00

abstract：:A cDNA of a type 7 adenylyl cyclase isoform was cloned from a bovine retinal pigment epithelium cDNA library using oligonucleotides developed to conserved regions common to mammalian adenylyl cyclases. A 6.7 kb mRNA of very high abundance was uniquely present on Northern blots containing mRNA or total RNA from the pig...

journal_title：FEBS letters

authors： Völkel H,Beitz E,Klumpp S,Schultz JE

更新日期：1996-01-15 00:00:00