Abstract:
:Endothelin-1 (ET-1) dose-dependently increased HIF1α expression in pulmonary artery smooth muscle cells (PASMCs). Inhibition of protein synthesis did not affect ET-1-induced HIF1α expression. The maximum effect of ET-1 was similar to that caused by proteasome inhibitor MG132. Further study indicates that ET-1 also dose-dependently stimulated calcineurin activation, specific calcineurin inhibitor cyclosporine A (CsA), abolished ET-1-induced HIF1α elevation, and reversed ET-1-induced RACK1 (receptor of activated protein kinase C 1) de-phosphorylation. Endothelin receptor A was found to specifically mediate the effects of ET-1. To examine whether RACK1 is particularly involved in proteasome-dependent HIF1α degradation, RACK1 was silenced by siRNA transfection. Cells lacking RACK1 exhibited significant elevation of HIF1α protein level. Taken together, our study suggests that ET-1 suppressed proteasome-dependent HIF1α degradation by calcineurin-dependent RACK1 de-phosphorylation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Li M,Liu Y,Jin F,Sun X,Li Z,Liu Y,Fang P,Shi H,Jiang Xdoi
10.1016/j.febslet.2012.08.036subject
Has Abstractpub_date
2012-11-02 00:00:00pages
3888-93issue
21eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00727-2journal_volume
586pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The intermediate in the reaction catalyzed by 4-chlorobenzoate dehalogenase from Pseudomonas sp. CBS3 was identified as 4-chlorobenzoyl-CoA. One component of 4-chlorobenzoate dehalogenase worked as a a 4-chlorobenzoyl-CoA ligase catalyzing the formation of 4-chlorobenzoyl-CoA from 4-chlorobenzoate, coenzyme A and ATP....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81265-a
更新日期:1991-09-23 00:00:00
abstract::Human D-type prostanoid (DP) and E-type prostanoid 2 (EP2) receptors are regarded as the most closely related receptors among prostanoid receptors. Although these receptors are generated by tandem duplication, their physiological outputs often oppose one another. In the present study, we extracted mutations occurring ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.02.006
更新日期:2015-03-12 00:00:00
abstract::The pluripotent nature of human embryonic stem cells (hESC) has attracted great interest in using them as a source of cells or tissue in cell therapy. However, in order to be used in regenerative medicine, the pluripotent hESC lines should be established and propagated according to good manufacturing practice quality ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2006.03.083
更新日期:2006-05-22 00:00:00
abstract::Ovulation is a complex process initiated by the surge of the pituitary luteinizing hormone (LH) that provokes the expression of specific genes. We report herein the isolation and characterization of an ovulation-associated, ovary-specific novel isoform of epoxide hydrolase 2 (Ephx2), Ephx2C. This variant is exclusivel...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.09.016
更新日期:2007-10-16 00:00:00
abstract::The eukaryotic Ssu72 factor is involved in several RNA biogenesis processes. It has phosphatase activity on the carboxy-terminal domain (CTD) of the major subunit of RNA polymerase II. The Kluyveromyces lactis Ssu72 (KlSsu72) shows in vitro phosphatase activity for the pNPP substrate, and this activity is inhibited by...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.06.044
更新日期:2013-08-19 00:00:00
abstract::An erythroid-specific enhancer was previously identified in the 3'-flanking region of the beta adult gene in chicken and duck, by transfection into AEV transformed chicken erythroblasts. Here we show that the duck enhancer is equally active in erythroid human K562 cells, presenting an embryonic/fetal program of globin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80103-0
更新日期:1988-07-18 00:00:00
abstract::Membrane proteins are usually solubilized in polar solvents by incorporation into micelles. Even for small membrane proteins these mixed micelles have rather large molecular masses, typically beyond 50000 Da. The NMR technique TROSY (transverse relaxation-optimized spectroscopy) has been developed for studies of struc...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(01)02742-9
更新日期:2001-08-31 00:00:00
abstract::The known members of inhibitor of growth (ING) gene family are considered as candidate tumor suppressor genes. ING4, a novel member of ING family, is recently reported to interact with tumor suppressor p53, p300 (a major component of histone acetyl transferase complexes), and p65(RelA) subunit of NF-kappaB. In this st...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.06.010
更新日期:2004-07-16 00:00:00
abstract::Quantitative modeling of intracellular processes often requires information about intracellular rate constants as well as the concentrations of low abundance species in individual cells. Single molecule imaging techniques offer not only new ways for obtaining such information but also the possibilities to test model-b...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2009.11.035
更新日期:2009-12-17 00:00:00
abstract::The metallochromic indicator murexide has been used to monitor calcium concentration changes during the dextran-induced, phosphatidylserine-dependent degranulation of rat peritoneal mast cells. The dextran-induced Ca2+-uptake showed an absolute dependence on the presence of phosphatidylserine. The extent of Ca2+-uptak...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80725-x
更新日期:1983-09-05 00:00:00
abstract::Plasminogen activator inhibitor-1 (PAI-1) increases injury in several liver, lung and kidney disease models. The objective of this investigation was to assess the effect of PAI-1 deficiency on cholestatic liver fibrosis and determine PAI-1 influenced fibrogenic mechanisms. We found that PAI-1(-/-) mice had less fibros...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.05.049
更新日期:2007-06-26 00:00:00
abstract::A hybrid protein from Neisseria meningitidis, which contains both a peroxiredoxin and a glutaredoxin domain, has been isolated. The enzyme was active in the reduction of various peroxides and dehydroascorbate in the presence of reduced glutathione. These findings suggest that both the peroxiredoxin and glutaredoxin do...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01156-6
更新日期:2003-11-06 00:00:00
abstract::The majority of cases of early-onset familial Alzheimer disease are caused by mutations in the recently identified presenilin 1 (PS1) gene, located on chromosome 14. PS1, a 467 amino acid protein, is predicted to be an integral membrane protein containing seven putative transmembrane domains and a large hydrophilic lo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00608-4
更新日期:1996-07-08 00:00:00
abstract::Hypoxic-ischaemic encephalopathy, resulting from asphyxia during birth, affects 2-3 in every 1000 term infants and depending on severity, brings about life-changing neurological consequences or death. This hypoxic-ischaemia (HI) results in a delayed neural energy failure during which the majority of brain injury occur...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12943
更新日期:2018-03-01 00:00:00
abstract::CHO SSF3 cells grow as a suspension culture in unmodified commercial medium with only low-molecular weight ingredients. Continuous serum-free culture unexpectedly induced expression of a low dihydrofolate reductase activity in the originally dhfr- CHO cells. Nevertheless, it was possible with methotrexate to induce am...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01328-8
更新日期:1995-12-27 00:00:00
abstract::The role of Arp3 in mouse development was investigated utilizing a gene trap mutation in the Arp3 gene. Heterozygous Arp3(WT/GT) mice are normal, however, homozygous Arp3(GT/GT) embryos die at blastocyst stage. Earlier embryonic stages appear unaffected by the mutation, probably due to maternal Arp3 protein. Mutant bl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.11.031
更新日期:2007-12-11 00:00:00
abstract::Measurement of the binding equilibrium for the interaction of alpha-actinin with F-actin is complicated by secondary reactions involving cross-linking and/or bundling of the actin filaments. To quantitate the initial binding event, we studied the interaction of the bacterially expressed actin-binding domain (ABD) of c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80434-6
更新日期:1994-02-21 00:00:00
abstract::A cytochrome c (cyt. c) was solubilized with Triton-X-100 and co-purified with cytochrome c oxidase from membranes of chemotrophically grown cells of Rhodopseudomonas capsulata. Cyt. c and cytochrome oxidase were separated on Sephadex G-50 columns. Antibodies against cytochrome c2 from the same bacterium did not cross...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80390-1
更新日期:1983-02-21 00:00:00
abstract::We have previously shown that Docetaxel-induced variable degrees of apoptosis in melanoma. In this report, we studied the beta-tubulin repertoire of melanoma cell lines and show that class III beta-tubulin expression correlated with Docetaxel-resistance. Sensitive cells showed low levels of class III beta-tubulin with...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.12.014
更新日期:2008-01-23 00:00:00
abstract::We recently showed that some of the enzymes underpinning cellulose solubilization by Ruminococcus albus 8 lack the conventional type of dockerin module characteristic of cellulosomal proteins and instead, bear an "X" domain of unknown function at their C-termini. We have now subcloned and expressed six X domains and s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.04.005
更新日期:2004-05-21 00:00:00
abstract::To identify genes whose expression is modulated by heregulin-beta1 (HRG), a regulatory polypeptide for mammary epithelial cells, we performed differential display screening of MCF-7 cell mRNA. One cDNA clone upregulated by HRG was identical to human ADP-ribosylation factor 3 (ARF3), a guanine nucleotide-binding protei...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02994-0
更新日期:2002-07-31 00:00:00
abstract::Hst1At (accession number AB018695) was identified from the Arabidopsis thaliana sequencing project on BAC T3F12, and the corresponding cDNA was isolated by reverse transcription-PCR. Southern blot analysis reveals a single copy of this gene. The cDNA encodes a root specific sulfate transporter of 649 amino acids. Hete...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01615-x
更新日期:2000-06-09 00:00:00
abstract::The critical urea concentration (C3*) which destabilizes the structure of bovine serum albumin and chymotrypsinogen was determined by UV difference spectroscopy. The increase of the relative content of mobile rotating water molecules in aqueous urea was formerly shown by millimeter spectroscopy [1]. The rise of rotato...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81152-p
更新日期:1993-01-04 00:00:00
abstract::The E-E* model for calcium pumping by the CaATPase of sarcoplasmic reticulum includes two distinct conformational states of the enzyme, E and E*. Exterior Ca2+ binds only to E and interior Ca2+ binds only to E*. Therefore, it is expected that there will be competition between the binding of calcium to the unphosphoryl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80077-g
更新日期:1991-01-14 00:00:00
abstract::The early response to bacterial inoculation has been investigated and two Arabidopsis genes, ap3.3a and ap4.3a have been characterized. The AP3.3A protein showed high identity to centrin, a ubiquitous cytoskeletal protein first identified in unicellular green alga. Amino-acid sequence analyses of the AP4.3A protein in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01015-1
更新日期:1998-09-04 00:00:00
abstract::Mesenchyme forkhead-1 (MFH-1), a winged helix/forkhead transcription factor, is expressed in developing cartilaginous tissues, kidney and arch arteries, and is essential for the normal development of the axial skeleton and aortic arch formation of mice. To investigate the possible role of MFH-1 in osteogenesis and ost...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01285-0
更新日期:2000-03-17 00:00:00
abstract::The odorant-induced accumulation of cAMP can be inhibited by antibodies directed against G alpha s/olf. In contrast, antibodies raised against G alpha i-subunits caused a strong enhancement of the odorant-induced cAMP accumulation. Western blotting and immunoelectron microscopy revealed the presence of both G alpha s/...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00368-8
更新日期:1998-04-24 00:00:00
abstract::The WRN protein is mutated in the chromosomally unstable Werner syndrome (WS) and the Nbs1 protein is mutated in Nijmegen breakage syndrome (NBS). The Nbs1 protein is an integral component of the M/R/N complex. Although WRN is known to interact with this complex in response to gamma-irradiation, the mechanism of actio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.01.028
更新日期:2005-02-28 00:00:00
abstract::The present one-drug-one-target paradigm in drug discovery has been considered partially responsible for the more-funding-less-drug predicament in modern pharmaceutical industry. To hit the multiple targets implicated in complex diseases, two strategies, based on multicomponent or single-ingredient, are conceivable. A...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2005.09.006
更新日期:2005-10-10 00:00:00
abstract::Transfer RNAs (tRNAs) are ancient macromolecules that have evolved under various environmental pressures as adaptors in translation in all forms of life but also towards alternative structures and functions. The present knowledge on both "canonical" and "deviating" signature motifs retrieved from vertical and horizont...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2009.11.050
更新日期:2010-01-21 00:00:00