Abstract:
:The role of Arp3 in mouse development was investigated utilizing a gene trap mutation in the Arp3 gene. Heterozygous Arp3(WT/GT) mice are normal, however, homozygous Arp3(GT/GT) embryos die at blastocyst stage. Earlier embryonic stages appear unaffected by the mutation, probably due to maternal Arp3 protein. Mutant blastocysts isolated at E3.5 fail to continue development in vitro, lack outgrowth of trophoblast-like cells in culture and express reduced levels of the trophoblast marker Cdx2, while markers for inner cell mass continue to be present. The recessive embryonic lethal phenotype indicates that Arp3 plays a vital role for early mouse development, possibly when trophoblast cells become critical for implantation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Vauti F,Prochnow BR,Freese E,Ramasamy SK,Ruiz P,Arnold HHdoi
10.1016/j.febslet.2007.11.031subject
Has Abstractpub_date
2007-12-11 00:00:00pages
5691-7issue
29eissn
0014-5793issn
1873-3468pii
S0014-5793(07)01175-1journal_volume
581pub_type
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