Abstract:
:Kinetic analyses of cpTat-mediated protein transport across the thylakoid membrane were conducted, revealing three important characteristics of this translocation pathway. First, transport via the cpTAT system displays a non-Michaelis-Menten, sigmoidal rate-substrate relationship with an apparent Hill coefficient of 1.80, indicative of positive homotropic cooperativity. Second, the presence of transport-incompetent substrates was found not to competitively inhibit the translocation of transport-competent substrates. However, the presence of low concentrations of transport-incompetent protein enhances the transport of wild type substrate. Together, these findings suggest that interaction between Tat machinery components and both transport-competent and transport-incompetent protein may elicit a cooperative effect on the translocation rate.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Alder NN,Theg SMdoi
10.1016/s0014-5793(03)00231-xkeywords:
subject
Has Abstractpub_date
2003-04-10 00:00:00pages
96-100issue
1-3eissn
0014-5793issn
1873-3468pii
S001457930300231Xjournal_volume
540pub_type
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