Abstract:
:Fluorescence techniques were used to study conformational changes that occur in inactive E. coli 30 S ribosomal subunits during activation by heating in 12 mM Mg2+. Activation is associated with movement of a fluorophore on the 3'-end of 16 S RNA into a less polar environment and towards a probe on the cysteine thiol of ribosomal protein S21. The conformational change causes an apparent decrease in distance between the probes from 59 to 52 A as determined by non-radiative energy transfer.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Odom OW,Stöffler G,Hardesty Bdoi
10.1016/0014-5793(84)81037-6subject
Has Abstractpub_date
1984-07-23 00:00:00pages
155-8issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(84)81037-6journal_volume
173pub_type
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