Involvement of phospholipase D in oxidative stress-induced necrosis of vascular smooth muscle cells.

abstract：:A bovine anterior pituitary polypeptide that stimulates the replication of rat and human adipocyte precursors has been purified. Its Mr is 44 000-53 000 and its isoelectric point is 9.8-10.3. While pituitary basic fibroblast growth factor is equally mitogenic on adipocyte precursors and skin fibroblasts, the polypepti...

journal_title：FEBS letters

authors： Lau DC,Roncari DA,Yip DK,Kindler S,Nilsen SG

更新日期：1983-03-21 00:00:00

abstract：:BSP-30K is a major acidic glycoprotein of bovine seminal plasma. It displays heparin-, gelatin-, and phospholipid-binding activities. BSP-30K binds to spermatozoa upon ejaculation and is thought to play a role in sperm capacitation. We have determined its amino acid sequence, disulfide bonds, and 0-glycosylation sites...

journal_title：FEBS letters

authors： Calvete JJ,Mann K,Sanz L,Raida M,Töpfer-Petersen E

更新日期：1996-12-09 00:00:00

abstract：:A cDNA clone encoding cholesterol side-chain cleavage cytochrome P450 (P450scc) was isolated from a rainbow trout ovarian follicle cDNA library. The cDNA contains an open reading frame of 1,542 nucleotides encoding a protein of 514 amino acids. The predicted amino acid sequence of trout P450scc shows 48% homology with...

journal_title：FEBS letters

authors： Takahashi M,Tanaka M,Sakai N,Adachi S,Miller WL,Nagahama Y

更新日期：1993-03-15 00:00:00

abstract：:RNA binding motif protein 5 (RBM5) is a candidate tumor suppressor gene. Recent studies showed that RBM5 functions as an alternative splicing regulator of apoptosis-related genes. Here, we identify DHX15 and PRP19, two spliceosome components, as novel RBM5-interacting partners. We then show that the G-patch domain of ...

journal_title：FEBS letters

authors： Niu Z,Jin W,Zhang L,Li X

更新日期：2012-04-05 00:00:00

abstract：:Sensitivity of mammalian cells to the bacterial toxin aerolysin is due to the presence at their surface of glycosylphosphatidyl inositol (GPI)-anchored proteins which act as receptors. Using a panel of mutants that are affected in the GPI biosynthetic pathway and Trypanosoma brucei variant surface glycoproteins, we sh...

journal_title：FEBS letters

authors： Abrami L,Velluz MC,Hong Y,Ohishi K,Mehlert A,Ferguson M,Kinoshita T,Gisou van der Goot F

更新日期：2002-02-13 00:00:00

abstract：:The action of substrate analogs containing the tetrazolyl group instead of the C-terminal carboxy group on the peptidase activity of carboxypeptidase A is studied. The analogs compete with the substrate for the secondary binding site thus showing activation phenomena. ...

journal_title：FEBS letters

authors： Kojro E,Willhardt I,Römbach A,Grzonka Z,Hermann P

更新日期：1987-02-09 00:00:00

abstract：:The present report provides evidence that, in A431 cells, interferon gamma (IFNgamma) induces the rapid (within 5 min), and reversible, tyrosine phosphorylation of the epidermal growth factor receptor (EGFR). IFNgamma-induced EGFR transactivation requires EGFR kinase activity, as well as activity of the Src-family tyr...

journal_title：FEBS letters

authors： Burova E,Vassilenko K,Dorosh V,Gonchar I,Nikolsky N

更新日期：2007-04-03 00:00:00

abstract：:The effects of protein disulfide isomerase (PDI) on the four structured des species that accumulate in the rate-determining steps of ribonuclease A folding were investigated at pH 8.0 and 15 degrees C. The results indicate that PDI catalyzes the conversion of the kinetically trapped intermediates, des-[26-84] and des-...

journal_title：FEBS letters

authors： Shin HC,Song MC,Scheraga HA

更新日期：2002-06-19 00:00:00

abstract：:The O2-dependent formation of side products during the oxidation of veratryl alcohol (VA) by lignin peroxidase has previously been proposed to start with the attack of H2O on the VA radical cation (VA*+). This initial reaction is unlikely since it would also lead to side product formation in the absence of O2, which i...

journal_title：FEBS letters

authors： ten Have R,Franssen MC

更新日期：2001-01-05 00:00:00

abstract：:We have purified two growth factors from dogfish brain and retina by using their binding affinity for heparin-Sepharose. These growth factors were eluted at 1 and 2 M NaCl similarly to those purified from bovine brain or retina. Their mitogenic activity was assayed in vitro on the same mammalian cells: bovine lens epi...

journal_title：FEBS letters

authors： Lagente O,Diry M,Courtois Y

更新日期：1986-07-07 00:00:00

abstract：:Ku, a heterodimer of 70- and 80-kDa subunits, plays a general role in the metabolism of DNA ends in eukaryotic cells, including double-strand DNA break repair, V(D)J recombination, and maintenance of telomeres. We have utilized the yeast two-hybrid system to identify Ku70-interacting proteins other than Ku80. Two reac...

journal_title：FEBS letters

authors： Song K,Jung D,Jung Y,Lee SG,Lee I

更新日期：2000-09-08 00:00:00

abstract：:We recently purified two closely related 33 kDa proteins from rat hepatic cytosol, designated bile acid binder I and II, which selectively bind bile acids with comparable affinity as glutathione S-transferase B. This work has now been extended to human liver in which we have identified a similar cytosolic binding acti...

journal_title：FEBS letters

authors： Stolz A,Sugiyama Y,Kuhlenkamp J,Kaplowitz N

更新日期：1984-11-05 00:00:00

abstract：:The yeast integral plasma membrane protein Ist2 belongs to a group of membrane proteins which are synthesized from localized mRNAs. The protein reaches the plasma membrane via the ER on a route operating independently of the classical secretory pathway. We have identified a complex peptide-sorting signal located at th...

journal_title：FEBS letters

authors： Franz A,Maass K,Seedorf M

更新日期：2007-02-06 00:00:00

abstract：:We compared oxidative DNA damage in strictly anaerobic Prevotella melaninogenica, aerotolerant anaerobic Bacteroides fragilis, and facultative anaerobic Salmonella typhimurium after exposure to O2 or H2O2. Using HPLC with electrochemical detection, we measured 8-hydroxydeoxyguanosine (8OHdG) as a damage marker. O2 ind...

journal_title：FEBS letters

authors： Takeuchi T,Nakaya Y,Kato N,Watanabe K,Morimoto K

更新日期：1999-05-07 00:00:00

abstract：:Five-lipoxygenase-activating protein (FLAP) is usually described as an essential protein to activate the leukotriene (LTs) synthesis via the 5-lipoxygenase pathway. In the enterocyte model HT29 cl.19A cell line, 5-lipoxygenase metabolism was found despite the lack of FLAP expression. Therefore HT29 cl.19A represents a...

journal_title：FEBS letters

authors： Battu S,Beneytout JL,Pairet M,Rigaud M

更新日期：1998-10-16 00:00:00

abstract：:Effects of sulfatide and its precursor galactosylceramide (gal-cer) on the kinetics of production of cytokines were studied. In human mononuclear leucocytes, gal-cer but not sulfatide induced significantly increased amounts of interleukin (IL)-1beta, IL-6 and tumor necrosis factor (TNF) mRNA. In phytohemagglutinin-sti...

journal_title：FEBS letters

authors： Bovin LF,Fredman P,Månsson JE,Buschard K,Bendtzen K

更新日期：1999-07-23 00:00:00

abstract：:Escherichia coli phosphofructokinase-2 (Pfk-2) is a homodimer whose subunits consist of a large domain and an additional beta-sheet that provides the interfacial contacts between the subunits, creating a beta-barrel flattened-like structure with the adjacent subunit's beta-sheet. To determine how the structural organi...

journal_title：FEBS letters

authors： Baez M,Babul J

更新日期：2009-06-18 00:00:00

abstract：:Proteins involved in protein translocation across the membrane of the endoplasmic reticulum assemble into different oligomeric complexes depending on their state of function. To analyse such membrane protein complexes we fractionated proteins of mammalian rough microsomes and analysed them using blue native PAGE and i...

journal_title：FEBS letters

authors： Wang L,Dobberstein B

更新日期：1999-09-03 00:00:00

abstract：:We studied the effect of high-fat diet on the expression and activation of the three caveolins in rat skeletal muscle and their association with the insulin signalling cascade. Initial response was characterized by increased signalling through Cav-1 and Cav-3 phosphorylation, suggesting that both participate in an ini...

journal_title：FEBS letters

authors： Gómez-Ruiz A,de Miguel C,Campión J,Martínez JA,Milagro FI

更新日期：2009-10-06 00:00:00

abstract：:Subclinical inflammation is a candidate etiological factor in the pathogenesis of metabolic syndrome and in the progression of atherosclerosis. A central role for activated macrophages has been elucidated recently as important regulators of the inflammatory process in atherosclerosis. Macrophage differentiation and fu...

journal_title：FEBS letters

authors： Szanto A,Roszer T

更新日期：2008-01-09 00:00:00

abstract：:T cells express two different types of voltage-independent Ca(2+)-activated K(+) channels with small (SK) and intermediate (IK) conductance that serve important roles in the activation of T lymphocytes. In contrast to the IK channels from T lymphocytes which are upregulated upon mitogen stimulation, SK channels of Jur...

journal_title：FEBS letters

authors： Jäger H,Adelman JP,Grissmer S

更新日期：2000-03-10 00:00:00

abstract：:Folding of nascent chains resembles the decoding of spoken language in that information is emitted as a unidirectional, one-dimensional string of elements, with higher structures and long-distance interactions emerging with time. Applying a "pseudolinguistic' analysis of structure to a set of all 36 possible six-stran...

journal_title：FEBS letters

authors： Gross M

更新日期：1996-07-29 00:00:00

abstract：:Nitrogen dioxide (NO2.) is often present in inhaled air and may be generated in vivo from nitric oxide. Exposure of human blood plasma to NO2. caused rapid losses of ascorbic acid, uric acid and protein thiol groups, as well as lipid peroxidation and depletions of alpha-tocopherol, bilirubin and ubiquinol-10. No incre...

journal_title：FEBS letters

authors： Halliwell B,Hu ML,Louie S,Duvall TR,Tarkington BK,Motchnik P,Cross CE

更新日期：1992-11-16 00:00:00

abstract：:Streptococci are a broad group of Gram-positive bacteria. This genus includes various human pathogens causing significant morbidity and mortality. Two of the most important human pathogens are Streptococcus pneumoniae (pneumococcus) and Streptococcus pyogenes (group A streptococcus or GAS). Streptococcal pathogens hav...

journal_title：FEBS letters

authors： Syed S,Viazmina L,Mager R,Meri S,Haapasalo K

更新日期：2020-08-01 00:00:00

abstract：:Bacterial cells containing the ner gene of phage Mu inserted into pBR322 express a binding activity with specificity for the left-end EcoRI.C fragment of Mu DNA. Crude extracts containing either Mu repressor or ner protein have been used to localize binding sites on TaqI subfragments of the EcoRI.C fragment. There are...

journal_title：FEBS letters

authors： Barlach S,Schumann W

更新日期：1983-06-27 00:00:00

abstract：:Hypermethylated in cancer, a new candidate tumor suppressor gene located in 17p13.3, encodes a protein with five Krüppel-like C2H2 zinc finger motifs and a N-terminal protein/protein interaction domain called broad complex, tramtrack and bric à brac/poxviruses and zinc finger domain. Hypermethylated in cancer appears ...

journal_title：FEBS letters

authors： Guerardel C,Deltour S,Leprince D

更新日期：1999-05-28 00:00:00

abstract：:Heparin Binding Hemagglutinin A (HBHA) is hitherto the sole virulence factor associated with tuberculosis dissemination from the lungs, the site of primary infection, to epithelial cells. We have previously reported the solution structure of HBHA, a dimeric and elongated molecule. Since oligomerisation of HBHA is asso...

journal_title：FEBS letters

authors： Esposito C,Carullo P,Pedone E,Graziano G,Del Vecchio P,Berisio R

更新日期：2010-03-19 00:00:00

abstract：:The rate of formation of diferric-transferrin has been studied using various combinations of Fe(II), Tf, Cp and h serum. When the reactants were added in a correct physiological sequence, ceruloplasmin and diluted human serum showed the fastest rate of saturation of transferrin. ...

journal_title：FEBS letters

authors： Chidambaram MV,Barnes G,Frieden E

更新日期：1983-08-08 00:00:00

abstract：:Two human mammary carcinoma cell variants, MCF-7/AZ and MCF-7/6, show the same composition in their glycosphingolipid-enriched microdomain (GEM) with regard to globo-series structures Gb3, Gb4, Gb5, monosialyl-Gb5, GM2, and cSrc and FAK. Both variants are non-invasive into collagen gel layer, and showed similar motili...

journal_title：FEBS letters

authors： Steelant WF,Kawakami Y,Ito A,Handa K,Bruyneel EA,Mareel M,Hakomori S

更新日期：2002-10-30 00:00:00

abstract：:Cyclic ADP-ribose (cADPR) is a natural metabolite of beta-NAD+ with a potent Ca2+-mobilizing activity in different cell types, including T-lymphocytes. We investigated (i) whether stimulation of T-lymphocytes with different agonists affects the intracellular concentration of cADPR, and (ii) whether the lymphocyte anti...

journal_title：FEBS letters

authors： da Silva CP,Schweitzer K,Heyer P,Malavasi F,Mayr GW,Guse AH

更新日期：1998-11-20 00:00:00