Rainbow trout ovarian cholesterol side-chain cleavage cytochrome P450 (P450scc). cDNA cloning and mRNA expression during oogenesis.

Abstract:

:A cDNA clone encoding cholesterol side-chain cleavage cytochrome P450 (P450scc) was isolated from a rainbow trout ovarian follicle cDNA library. The cDNA contains an open reading frame of 1,542 nucleotides encoding a protein of 514 amino acids. The predicted amino acid sequence of trout P450scc shows 48% homology with that of human, and 46% homology with that of rat, bovine and pig. P450scc activity was confirmed by transfected COS-1 monkey kidney tumour cells with an expression vector for trout P450scc cDNA and subsequent detection of conversion from 25-hydroxycholesterol to pregnenolone by radioimmunoassay. The cDNA only hybridized to a single 1.8 kb RNA transcript. The transcript was not found in early vitellogenic follicles, barely detected in postvitellogenic follicles, and abundant in postovulatory follicles.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Takahashi M,Tanaka M,Sakai N,Adachi S,Miller WL,Nagahama Y

doi

10.1016/0014-5793(93)80034-r

subject

Has Abstract

pub_date

1993-03-15 00:00:00

pages

45-8

issue

1-2

eissn

0014-5793

issn

1873-3468

pii

0014-5793(93)80034-R

journal_volume

319

pub_type

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