The primary structure of BSP-30K, a major lipid-, gelatin-, and heparin-binding glycoprotein of bovine seminal plasma.

abstract：:T helper 1 (Th1) cell fate is induced by overlapping signaling pathways, whose kinetic principles and regulatory motifs are largely unknown. We identified a simple positive feedback loop in the STAT4 signaling pathway, whereby activation by IL-12 leads to the increased expression in IL-12 receptor. A computational ana...

journal_title：FEBS letters

authors： Becskei A,Grusby MJ

更新日期：2007-11-13 00:00:00

abstract：:Measurements of the effects of NH3/NH4+ on glucose-induced electrical activity in beta-cells from microdissected mouse islets of Langerhans and on intracellular pH in single collagenase-isolated islets pre-loaded with a fluorescent pH probe were performed and are reported here. Application of NH3/NH4+ (15 mM) in the p...

journal_title：FEBS letters

authors： Rosario LM,Rojas E

更新日期：1986-05-05 00:00:00

abstract：:We have studied the action of an alpha-latrotoxin (alpha-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC12 cells as well as on [14C]GABA release from synaptosomes. ...

journal_title：FEBS letters

authors： Grishin EV,Himmelreich NH,Pluzhnikov KA,Pozdnyakova NG,Storchak LG,Volkova TM,Woll PG

更新日期：1993-12-27 00:00:00

abstract：:In mouse mammary epithelial cells in primary culture, mechanical stimulation of a cell induced in other cells within the same colony a short depolarization of less than 15 mV with a duration of 1-8 s and a subsequent, prominent hyperpolarization of 6 mV lasting 10-40 s. Epidermal growth factor induces a spontaneous hy...

journal_title：FEBS letters

authors： Enomoto K,Furuya K,Maeno T,Edwards C,Oka T

更新日期：1987-10-19 00:00:00

abstract：:The gene for the extracellular ribonuclease of B. pumilus KMM62 (RNase Bp) has been cloned and sequenced. The structural gene for this enzyme is similar to those of the extracellular ribonucleases of B. intermedius 7P (binase) and B. amyloliquefaciens H2 (barnase), as are the regulatory regions of binase and RNase Bp....

journal_title：FEBS letters

authors： Znamenskaya LV,Gabdrakhmanova LA,Chernokalskaya EB,Leshchinskaya IB,Hartley RW

更新日期：1995-01-02 00:00:00

abstract：:Cytosine in nucleic acids can be converted into N4-aminocytosine by treatment with a mixture of hydrazine and bisulfite. The hydrazino group thus formed at position 4 of the pyrimidine ring can be linked to a sulhydryl group in proteins by the use of bromopyruvate as a linker. Successful use of this scheme of chemical...

journal_title：FEBS letters

authors： Nitta N,Kuge O,Yui S,Tsugawa A,Negishi K,Hayatsu H

更新日期：1984-01-23 00:00:00

abstract：:32 consensus patterns for a set of functional regions and structural motifs in protein sequences were constructed. The pattern definition is heuristic and based on 11 selected steric and physicochemical properties. By comparison with these patterns, it was possible to identify, without false detection, 1532 sites in 8...

journal_title：FEBS letters

authors： Bork P

更新日期：1989-10-23 00:00:00

abstract：:The kinetic constants of internalization of asialoorosomucoid were determined for normal and jejuno-ileal by-passed rat hepatocytes. In by-passed rats the maximum velocity of asialoorosomucoid internalization is decreased 3-fold, without any modification of apparant constant of internalization. Moreover, the rate cons...

journal_title：FEBS letters

authors： Serbource-Goguel N,Dodeur M,Borel B,Feger J,Agneray J,Durand G

更新日期：1985-04-22 00:00:00

abstract：:Cleavage of the envelope glycoprotein precursor gp160 of HIV-1 is a prerequisite for the infectivity of HIV-1, and occurs at least in part before gp160 reaches the cell surface. Kexin/subtilisin-related endopeptidases are proposed enzyme candidates for this intracellular processing. In this study, we reveal the possib...

journal_title：FEBS letters

authors： Okumura Y,Yano M,Murakami M,Mori S,Towatari T,Kido H

更新日期：1999-01-08 00:00:00

abstract：:The effects of insulin and IGF-I on the cell surface quantities of GLUT1, GLUT3 and GLUT4 glucose transporters in L6 myotubes were determined with the exofacial bis-mannose phololabel (ATB-BMPA). In basal cells, an equal molar quantity of each transporter isoform was found at the cell surface. Insulin stimulated the t...

journal_title：FEBS letters

authors： Wilson CM,Mitsumoto Y,Maher F,Klip A

更新日期：1995-07-10 00:00:00

abstract：:When isolated reaction centres of photosystem II are subjected to photoinhibitory illumination, a 41 kDa SDS-PAGE band is observed under all experimental conditions. The same band is also found, together with lower molecular weight fragments of the D1 protein, in whole thylakoids and in all PSII sub-particles investig...

journal_title：FEBS letters

authors： Barbato R,Friso G,Rigoni F,Frizzo A,Giacometti GM

更新日期：1992-09-07 00:00:00

abstract：:We have investigated the activity of STAT family members throughout a mammary developmental cycle. Transcripts for Stat 5 were upregulated during pregnancy whilst STAT1 and STAT3 mRNAs were expressed at constant levels. DNA binding complexes containing both STAT5a and 5b showed differing affinities for two naturally o...

journal_title：FEBS letters

authors： Philp JA,Burdon TG,Watson CJ

更新日期：1996-10-28 00:00:00

abstract：:A mutant of Escherichia coli has been described that produces an altered form of penicillin-binding protein 5 which still binds penicillin but is unable to catalyse the release of the bound penicilloyl moiety. We show that the mutation is caused by a single nucleotide transition that results in a change from glycine a...

journal_title：FEBS letters

authors： Broome-Smith J,Spratt BG

更新日期：1984-01-09 00:00:00

abstract：:The role of the RNA-binding protein human antigen R (HuR) in hepatocarcinogenesis is still elusive. By employing short hairpin (sh)RNA-dependent knockdown approach, we demonstrate that lymphotoxin α (LTα) is a target of posttranscriptional gene regulation by HuR in hepatocellular carcinoma (HepG2) cells. Consequently,...

journal_title：FEBS letters

authors： Steinmeyer N,Doller A,Biyanee A,Brauss T,Schmid T,Pfeilschifter J,Eberhardt W

更新日期：2015-07-08 00:00:00

abstract：:The molecular organisation of the Prochlorococcus marinus rnpB gene and the catalytic activity of the encoded RNA were characterised. Kinetic parameters for several pre-tRNA substrates were comparable to those from other eubacterial RNase P RNAs, although unusually high cation concentrations were required. The CCA-end...

journal_title：FEBS letters

authors： Hess WR,Fingerhut C,Schön A

更新日期：1998-07-17 00:00:00

abstract：:The amino-terminal part of prion protein (PrP), containing a series of octapeptide repeats with the consensus sequence PHGGGWGQ, has been implicated in the binding of copper ion. This region possesses amino acid residues susceptible to oxidation, such as histidine, lysine, arginine and proline. In this study, we have ...

journal_title：FEBS letters

authors： Shiraishi N,Nishikimi M

更新日期：2002-01-30 00:00:00

abstract：:Despite significant increases in circulating concentrations of lipid fuels (triacylglycerol, non-esterified fatty acids (NEFA) and ketone bodies) in late-pregnant rats sampled in the fed (absorptive) state, cardiac and skeletal muscle active pyruvate dehydrogenase (PDHa) activities remained comparable with those obser...

journal_title：FEBS letters

authors： Sugden MC,Holness MJ

更新日期：1993-04-26 00:00:00

abstract：:We have used a competitive enzyme-linked immunoassay with a panel of monoclonal antibodies to probe the topography of the membrane-bound form of apolipoprotein B (apo B) in rabbit microsomes. All epitopes investigated were found to be expressed at the cytosolic side of the microsomal membrane under conditions in which...

journal_title：FEBS letters

authors： Wilkinson J,Higgins JA,Groot PH,Gherardi E,Bowyer DE

更新日期：1992-06-08 00:00:00

abstract：:Polyhydroxyalkanoate (PHA) synthases catalyze the polymerization reaction of the acyl moiety of hydroxyacyl-coenzyme A into polyester. The catalytic subunit PhaC of PHA synthase has the PhaC box sequence at the active site that is typically described as G-X-C-X-G-G (X is an arbitrary amino acid), and cysteine is an ac...

journal_title：FEBS letters

authors： Nambu Y,Ishii-Hyakutake M,Harada K,Mizuno S,Tsuge T

更新日期：2020-02-01 00:00:00

abstract：:A Synechococcus PCC7942 mutant in which the psbO gene was inactivated by insertion of a chloramphenicol interposon and which did not contain any detectable manganese stabilizing protein in immunoblot experiments, was constructed. Such a Synechococcus mutant was able to grow under photoautotrophic conditions. Isolated ...

journal_title：FEBS letters

authors： Bockholt R,Masepohl B,Pistorius EK

更新日期：1991-12-02 00:00:00

abstract：:We have previously shown that the Mycoplasma mycoides glycine tRNA (anticodon UCC) effectively reads the codons GGU and GGC in violation of the classic codon reading rules. We have attempted to elucidate what structural elements in this tRNA molecule confer this translational property and in the course of this investi...

journal_title：FEBS letters

authors： Claesson C,Samuelsson T,Lustig F,Borén T

更新日期：1990-10-29 00:00:00

abstract：:In hydra the neuropeptide head activator (HA) is responsible for head-specific growth and differentiation processes. The effects of HA are mediated by high and low affinity receptors on hydra cells. In the current study HA receptors from a multi-headed mutant of Chlorohydra viridissima were solubilized from the membra...

journal_title：FEBS letters

authors： Christians S,Neubauer KH,Ulrich H

更新日期：1993-01-25 00:00:00

abstract：:The uncoupling protein-3 (UCP3) is a mitochondrial protein expressed mainly in skeletal muscle. Among several hypotheses for its physiological function, UCP3 has been proposed to prevent excessive production of reactive oxygen species. In the present study, we evaluated the effect of an oxidative stress induced by hyp...

journal_title：FEBS letters

authors： Flandin P,Donati Y,Barazzone-Argiroffo C,Muzzin P

更新日期：2005-06-20 00:00:00

abstract：:Zinc (Zn) deficiency is often associated with low plasma vitamin A (retinol) concentrations. It has been suggested that the reduction in plasma retinol is secondary to reduced liver retinol binding protein (RBP) synthesis. In the present study, RBP expression was determined in HepG2 cells cultured in either Zn adequat...

journal_title：FEBS letters

authors： Satre MA,Jessen KA,Clegg MS,Keen CL

更新日期：2001-03-02 00:00:00

abstract：:The membrane-membrane fusion during fertilization of oocyte by spermatozoa is believed to be mainly mediated by so called "fusion proteins". In the present study we have tried to demonstrate that beside the proteins, lipid components of membrane may play an important role in fusion of oocyte with spermatozoa. Conventi...

journal_title：FEBS letters

authors： Atif SM,Hasan I,Ahmad N,Khan U,Owais M

更新日期：2006-04-17 00:00:00

abstract：:For good function, membrane lipids have to be arranged appropriately and be in the correct physical state. In poikilotherms, exposure to cold stress or heat shock can alter membrane properties such that, unless they are corrected quickly, damage and, possibly, death can result. Low temperature stress is countered by m...

journal_title：FEBS letters

authors： Guschina IA,Harwood JL

更新日期：2006-10-09 00:00:00

abstract：:Oil crops are in increasing demand both for food and as renewable sources of chemicals. It is therefore vital to understand how oil accumulation is regulated. Different ways of obtaining such information are discussed with an emphasis on metabolic control analysis. The usefulness of the latter has been well-illustrate...

journal_title：FEBS letters

authors： Harwood JL,Guschina IA

更新日期：2013-06-27 00:00:00

abstract：:Nine of the cbi genes from the 17.5 kb cob operon of Salmonella typhimurium previously shown by genetic studies to be involved in the biosynthesis of cobinamide from precorrin-2, have been subcloned and expressed in Escherichia coli. Seven of the gene products were found in the soluble fraction of cell lysates and hav...

journal_title：FEBS letters

authors： Roessner CA,Warren MJ,Santander PJ,Atshaves BP,Ozaki S,Stolowich NJ,Iida K,Scott AI

更新日期：1992-04-13 00:00:00

abstract：:Plastidic type I signal peptidase 1 (Plsp1) is necessary for proper chloroplast development although its mode of action is not completely understood. Here, we demonstrate by immunoblotting and electron microscopy immunolocalization that Plsp1 is evenly distributed in the envelope and thylakoids of developing chloropla...

journal_title：FEBS letters

authors： Shipman RL,Inoue K

更新日期：2009-03-04 00:00:00

abstract：:In this study, we demonstrated that laminin-1 (LM-1) induces neurite outgrowth and enhances the expression of neurofilament-L and MAP2 in human bone marrow mesenchymal stem cells (MSCs). The c-Jun transcription factor was strongly activated by LM-1 during neurite induction. Suppression of c-Jun inhibited the expressio...

journal_title：FEBS letters

authors： Mruthyunjaya S,Rumma M,Ravibhushan G,Anjali S,Padma S

更新日期：2011-06-23 00:00:00