Abstract:
:The gene for the extracellular ribonuclease of B. pumilus KMM62 (RNase Bp) has been cloned and sequenced. The structural gene for this enzyme is similar to those of the extracellular ribonucleases of B. intermedius 7P (binase) and B. amyloliquefaciens H2 (barnase), as are the regulatory regions of binase and RNase Bp. The regulatory region of the barnase gene, however, is quite different from the other two. In the promoter of the genes for binase and RNase Bp, but not in that for barnase, is a region similar to the Pho box of E. coli. We have established that inorganic phosphate suppresses the synthesis of the binase and RNase Bp, but does not effect the synthesis of barnase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Znamenskaya LV,Gabdrakhmanova LA,Chernokalskaya EB,Leshchinskaya IB,Hartley RWdoi
10.1016/0014-5793(94)01316-ssubject
Has Abstractpub_date
1995-01-02 00:00:00pages
16-8issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(94)01316-Sjournal_volume
357pub_type
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