The extracellular processing of HIV-1 envelope glycoprotein gp160 by human plasmin.

abstract：:The inhibitory glycine receptor (GlyR) is a ligand-gated chloride channel protein which displays developmental heterogeneity in the mammalian central nervous system. Here we describe 2 novel cDNA variants of the rat GlyR alpha 2 subunit and demonstrate that alternative splicing generates these 2 isoforms. The deduced ...

journal_title：FEBS letters

authors： Kuhse J,Kuryatov A,Maulet Y,Malosio ML,Schmieden V,Betz H

更新日期：1991-05-20 00:00:00

abstract：:A pulsed field gradient three-dimensional isotope-filtered 13C HMQC-NOESY experiment has been developed to characterize intermolecular contacts in a 37 kDa macromolecular ternary complex consisting of uniformly 13C labeled trp-repressor, its natural abundance co-repressor, L-tryptophan, and natural abundance operator ...

journal_title：FEBS letters

authors： Lee W,Revington MJ,Arrowsmith C,Kay LE

更新日期：1994-08-15 00:00:00

abstract：:A cDNA clone encoding cytochrome P-450(11)beta of rat adrenal has been cloned and sequenced using a bovine P-450(11)beta cDNA insert (pcP-450(11 beta)-2; (1987) J. Biochem. 102, 559-568) as a probe. The nucleotide sequence contains an open reading frame sufficient to encode the entire amino acid sequence of a P-450(11...

journal_title：FEBS letters

authors： Nonaka Y,Matsukawa N,Morohashi,Omura T,Ogihara T,Teraoka H,Okamoto M

更新日期：1989-09-11 00:00:00

abstract：:When rat pancreatic islets were incubated with 10(-8) M arginine vasopressin in the presence of 15 mM glucose there was a pronounced inhibition of insulin release in comparison with controls. This inhibitory effect appeared to be specific for vasopressin since it was antagonised by vasopressin antibody. Moreover, pert...

journal_title：FEBS letters

authors： Khalaf LJ,Taylor KW

更新日期：1988-04-11 00:00:00

abstract：:In contrast to the preponderance of affected males in families with X-linked mental retardation, Rett syndrome (RTT) is a neurological disorder occurring almost exclusively in females. The near complete absence of affected males in RTT families has been explained by the lethal effect of an X-linked gene mutation in he...

journal_title：FEBS letters

authors： Orrico A,Lam C,Galli L,Dotti MT,Hayek G,Tong SF,Poon PM,Zappella M,Federico A,Sorrentino V

更新日期：2000-09-22 00:00:00

abstract：:The STARD1 subfamily of 'START' lipid trafficking proteins can reduce macrophage lipid content and inflammatory status (STARD1; StAR), and traffic cholesterol from endosomes (STARD3/MLN64). During macrophage differentiation, STARD1 mRNA and protein increase with sterol content, while the reverse is true for STARD3. St...

journal_title：FEBS letters

authors： Borthwick F,Taylor JM,Bartholomew C,Graham A

更新日期：2009-04-02 00:00:00

abstract：:Gene expression is controlled through a complex interplay among mRNAs, non-coding RNAs and RNA-binding proteins (RBPs), which all assemble along with other RNA-associated factors in dynamic and functional ribonucleoprotein complexes (RNPs). To date, our understanding of RBPs is largely limited to proteins with known o...

journal_title：FEBS letters

authors： Faoro C,Ataide SF

更新日期：2014-10-16 00:00:00

abstract：:Saturable binding sites for the labelled calcium antagonist (+/-)[3H]nimodipine were found in guinea-pig hind limb skeletal muscle homogenates. Binding sites were enriched in a microsomal pellet by differential centrifugation of the homogenate. [3H]Nimodipine binding (Kd = 1.5 +/- 0.03 nM, Bmax = 2.1 +/- 0.25 pmol/pro...

journal_title：FEBS letters

authors： Ferry DR,Glossmann H

更新日期：1982-11-08 00:00:00

abstract：:Effects of amino acid substitutions in the first extracellular loop region of the delta- and mu-opioid receptors were examined. Substitution of lysine-108 of the delta-receptor (delta K108) with asparagine improved affinity to [D-Ala2,MePhe4,Gly-ol5]enk ephalin (DAGO), a mu-selective peptide agonist, to be comparable ...

journal_title：FEBS letters

authors： Fukuda K,Terasako K,Kato S,Mori K

更新日期：1995-10-09 00:00:00

abstract：:Di(1,N6-ethenoadenosine)5',5'''-P1,P4-tetraphosphate, epsilon-(Ap4A), a fluorescent analog of Ap4A has been synthesized by reaction of 2-chloroacetaldehyde with Ap4A. At neutral pH this Ap4A analog presents characteristics maxima at 265 and 274 nm, shoulders at ca 260 and 310 nm and moderate fluorescence (lambda exc 3...

journal_title：FEBS letters

authors： Rotilán P,Ramos A,Pintor J,Torres M,Miras-Portugal MT

更新日期：1991-03-25 00:00:00

abstract：:The effects of anaphylatoxin C3a on human neutrophils were studied in comparison with C5a. Both peptides induced a transient shape change response and a respiratory burst. In both cases C3a was 50- to 100-times less potent than C5a. A marked chemotactic response with bimodal concentration dependence was obtained with ...

journal_title：FEBS letters

authors： Ehrengruber MU,Geiser T,Deranleau DA

更新日期：1994-06-13 00:00:00

abstract：:When cultured porcine aortic endothelial cells (ECs) were incubated with porcine big endothelin-1 (bit ET-1(1-39)), there was a time-dependent increase in immunoreactive (IR)-ET in the culture supernatant, in addition to an endogenous IR-ET release fron the cells. Reverse-phase HPLC of the culture supernatant revealed...

journal_title：FEBS letters

authors： Ikegawa R,Matsumura Y,Tsukahara Y,Takaoka M,Morimoto S

更新日期：1991-11-18 00:00:00

abstract：:The products of the isiAB operon are a chlorophyll antenna protein (IsiA) and flavodoxin (IsiB), which accumulate in cyanobacteria grown under iron starvation conditions. Here we show that strong light triggers de-repression of isiAB transcription and leads to IsiA and flavodoxin accumulation under iron replete condit...

journal_title：FEBS letters

authors： Havaux M,Guedeney G,Hagemann M,Yeremenko N,Matthijs HC,Jeanjean R

更新日期：2005-04-25 00:00:00

abstract：:The type 1 ribosome-inactivating protein (RIP) saporin 5 isolated from seeds of Saponaria officinalis L. strongly inhibited translation carried out by Vicia sativa L. purified ribosomes. The toxin multidepurinated V. sativa rRNA, which upon treatment with acid aniline releases several RNA fragments including an RNA fr...

journal_title：FEBS letters

authors： Iglesias R,Arias FJ,Rojo MA,Escarmis C,Ferreras JM,Girbés T

更新日期：1993-07-05 00:00:00

abstract：:The presence of synaptic proteins involved in excitation/secretion coupling was examined in ten small cell lung cancer lines. N-Type calcium channels (omega-conotoxin receptors), synaptotagmin (p65) and syntaxin (HPC-1) were detected in eight. Co-immunoprecipitation experiments indicated that syntaxin can form a compl...

journal_title：FEBS letters

authors： David P,el Far O,Martin-Mouto N,Poupon MF,Takahashi M,Seagar MJ

更新日期：1993-07-12 00:00:00

abstract：:In this work, we have spectroscopically characterised CYP157C1 from Streptomyces coelicolor A3(2) which has the motif E(297)QSLW(301) rather than the invariant EXXR motif in the P450 K-helix. Site-directed mutagenesis of native E(297)QSLW(301) in CYP157C1 to E(297)ESLR(301) or E(297)QSRW(301) both containing standard ...

journal_title：FEBS letters

authors： Rupasinghe S,Schuler MA,Kagawa N,Yuan H,Lei L,Zhao B,Kelly SL,Waterman MR,Lamb DC

更新日期：2006-11-27 00:00:00

abstract：:Different hydrophobic glycoproteins are associated to native biliary vesicles, which are the major carrier of biliary cholesterol. Some of these proteins promote cholesterol crystallization, a key step in cholesterol gallstone formation. This study was specifically conducted to identify the 130 kDa biliary vesicle-ass...

journal_title：FEBS letters

authors： Núñez L,Amigo L,Rigotti A,Puglielli L,Mingrone G,Greco AV,Nervi F

更新日期：1993-08-23 00:00:00

abstract：:Several apolipoproteins including apoE and apoA-I are known to be associated with amyloid beta-peptide, a major component of senile plaques in Alzheimer's disease. In the present study the interaction between three human amphipathic apolipoproteins apoE3, apoA-I and apoA-II and immobilized amyloid beta-peptide (1-40) ...

journal_title：FEBS letters

authors： Shuvaev VV,Siest G

更新日期：1996-03-25 00:00:00

abstract：:Gemin5 acts as a U1 small nuclear RNA (snRNA)-binding protein in U1 small nuclear ribonucleic protein (snRNP) biogenesis. Here, we report a role for Gemin5 in unassembled U1 snRNP disposal under survival of motor neuron (SMN) protein-deficient conditions. We demonstrate that non-Sm protein-associated U1 snRNA and U1A ...

journal_title：FEBS letters

authors： Jiang D,Zou X,Zhang C,Chen J,Li Z,Wang Y,Deng Z,Wang L,Chen S

更新日期：2018-04-01 00:00:00

abstract：:Protein kinase A (PKA) signaling is essential for growth and virulence of the fungal pathogen Aspergillus fumigatus. Little is known concerning the regulation of this pathway in filamentous fungi. Employing liquid chromatography-tandem mass spectroscopy, we identified novel phosphorylation sites on the regulatory subu...

journal_title：FEBS letters

authors： Shwab EK,Juvvadi PR,Waitt G,Soderblom EJ,Moseley MA,Nicely NI,Steinbach WJ

更新日期：2017-11-01 00:00:00

abstract：:Na(+)/H(+) antiporters are ubiquitous membrane proteins and play an important role in cell homeostasis. We amplified a gene encoding a member of the monovalent cation:proton antiporter-2 (CPA2) family (TC 2.A.37) from the Thermus thermophilus genome and expressed it in Escherichia coli. The gene product was identified...

journal_title：FEBS letters

authors： Furrer EM,Ronchetti MF,Verrey F,Pos KM

更新日期：2007-02-06 00:00:00

abstract：:The characteristics of renal transport of L-alanine by luminal-membrane vesicles from proximal straight tubules (pars recta) of rabbit kidney were investigated. The following picture emerges from transport studies. Two electrogenic and Na+ requiring systems confined to this region of the nephron exist for the transpor...

journal_title：FEBS letters

authors： Vorum H,Jessen H,Jørgensen KE,Sheikh MI

更新日期：1988-01-18 00:00:00

abstract：:An abundant matrix protein was purified under native conditions from adult bovine tendon and identified as cartilage oligomeric matrix protein (COMP) by immunochemical crossreaction, amino acid sequence identity of tryptic peptides derived from both N- and C-terminal regions, and structure revealed by electron microsc...

journal_title：FEBS letters

authors： DiCesare P,Hauser N,Lehman D,Pasumarti S,Paulsson M

更新日期：1994-11-07 00:00:00

abstract：:In aqueous suspensions of purple membranes (pH 10.2, 0.4 M KCl) an intermediate having an absorption maximum at 570-575 nm (at -196 degrees C) was produced by first heating the M intermediate up to -30 degrees C and then stabilizing it by subsequent cooling to -60 degrees C. We suggest that this species is the interme...

journal_title：FEBS letters

authors： Balashov SP,Imasheva ES,Litvin FF,Lozier RH

更新日期：1990-10-01 00:00:00

abstract：:Interaction of the widely expressed Fas with its membrane-bound ligand (FasL) leads to rapid cell death via apoptosis. To avoid pathological tissue damage, the activity of FasL requires tight regulation. Here, we report that the Src homology 3 (SH3) domain of Fyn binds to the proline-rich cytoplasmic region of FasL. B...

journal_title：FEBS letters

authors： Hane M,Lowin B,Peitsch M,Becker K,Tschopp J

更新日期：1995-10-16 00:00:00

abstract：:The concept of critical period plasticity in rat visual cortex has been studied in terms of changes in the level of filamentous actin and of the 200 kDa neurofilament polypeptide. Our results suggest that the postnatal developmental profile of filamentous actin is affected by visual experience, as a consequence of eye...

journal_title：FEBS letters

authors： Nona SN,Trowell SC,Cronly-Dillon JR

更新日期：1985-07-01 00:00:00

abstract：:In Saccharomyces cerevisiae, four proteins, Shu1, Shu2, Psy3 and Csm2, form a stable SHU-complex both in vivo and in vitro. These proteins are involved in the early stages of the homologous recombination DNA damage repair process. In this paper, the crystal structure of the Psy3-Csm2 sub-complex is presented at 1.8Å r...

journal_title：FEBS letters

authors： She Z,Gao ZQ,Liu Y,Wang WJ,Liu GF,Shtykova EV,Xu JH,Dong YH

更新日期：2012-07-30 00:00:00

abstract：:We tested the effect of melatonin on membrane fluidity in microsomes of a rat liver model in which lipid peroxidation was induced by the addition of FeCl3, ADP and NADPH. Membrane fluidity was monitored using fluorescence spectroscopy and lipid peroxidation was estimated by quantifying malonaldehyde (MDA)+4-hydroxyalk...

journal_title：FEBS letters

authors： García JJ,Reiter RJ,Guerrero JM,Escames G,Yu BP,Oh CS,Muñoz-Hoyos A

更新日期：1997-05-26 00:00:00

abstract：:Stimulation of mas-oncogene transfected 401L-C3 cells by angiotensins leads to the production of inositol phosphates. This response shows dose dependence, and has an apparent rank order of potency angiotensin III greater than or equal to angiotensin II much greater than angiotensin I. Preincubation with 12-O-tetradeca...

journal_title：FEBS letters

authors： Jackson TR,Hanley MR

更新日期：1989-07-17 00:00:00

abstract：:The lack of trehalose accumulation in most plant species has been partly attributed to the presence of an active trehalase. Although trehalose synthesis enzymes are thought to be cytosolic, and previous studies have indicated that trehalase activity is extracellular, the exact location of the enzyme has not yet been e...

journal_title：FEBS letters

authors： Frison M,Parrou JL,Guillaumot D,Masquelier D,François J,Chaumont F,Batoko H

更新日期：2007-08-21 00:00:00