Abstract:
:A pulsed field gradient three-dimensional isotope-filtered 13C HMQC-NOESY experiment has been developed to characterize intermolecular contacts in a 37 kDa macromolecular ternary complex consisting of uniformly 13C labeled trp-repressor, its natural abundance co-repressor, L-tryptophan, and natural abundance operator DNA. The pulse scheme makes use of pulsed field gradients for the removal of artifacts and dephasing of unwanted magnetization during isotope filtering, and employs a strategy to minimize the time that magnetization resides in the transverse plane. The experiment provides solely intermolecular NOE contacts between protons of the labeled protein and protons of the unlabeled species, and has proven to be especially useful in eliminating ambiguities between intra- and intermolecular NOEs in the isotope-edited 3D 13C HMQC-NOESY spectrum of the complex.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lee W,Revington MJ,Arrowsmith C,Kay LEdoi
10.1016/0014-5793(94)00740-3subject
Has Abstractpub_date
1994-08-15 00:00:00pages
87-90issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(94)00740-3journal_volume
350pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Apoptotic proteases cleave and inactivate survival signaling molecules such as Akt/PKB, phospholipase C (PLC)-gamma1, and Bcl-2. We have found that treatment of A431 cells with tumor necrosis factor-alpha in the presence of cycloheximide resulted in the cleavage of epidermal growth factor receptor (EGFR) as well as th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02167-6
更新日期:2001-02-23 00:00:00
abstract::The Fanconi anemia (FA) pathway, of which the FANCD2 protein is a key component, plays crucial roles in the maintenance of hematopoietic stem cells and suppression of carcinogenesis. However, the function of FANCD2 remains unclear. Here, we report that FANCD2 is a novel and specific substrate of caspase 3. Cleavage of...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.08.027
更新日期:2014-10-16 00:00:00
abstract::The present study was focused on the regulation of ecdysteroidogenesis in the Y-organ of Scylla serrata during molting cycle. A strong expression of molt-inhibiting hormone (MIH) and phosphorylation of ERK was predominantly observed in the postmolt and intermolt stages of Y-organs, whereas protein kinase C, steroidoge...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.09.029
更新日期:2007-11-13 00:00:00
abstract::Tau proteins are abnormally phosphorylated in Alzheimer's disease. Pathological Tau proteins named PHF-Tau 55, PHF-Tau 64, and PHF-Tau 69, are the main constituents of the paired helical filaments (PHF). When treating SKNSH-SY 5Y cells with okadaic acid (OA), Tau 55 protein was clearly induced whereas Tau 64 protein w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01361-4
更新日期:1995-01-03 00:00:00
abstract::Bcl-xL, a close homolog of Bcl2, is an important regulator of apoptosis and is overexpressed in human cancer. Phosphorylation of Bcl-xL can be induced by microtubule-damaging drugs such as taxol or 2-methoxyestradiol (2-ME). By site-directed mutagenesis studies, we have identified that serine 62 is the necessary site ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00131-5
更新日期:2003-03-13 00:00:00
abstract::Olfactory cilia contain cyclic nucleotide-gated and Ca2+-dependent Cl- conductances that underlie excitatory chemotransduction, and a Ca2+-dependent K+ (KCa) conductance, apparently involved in inhibitory transduction. Previous single-channel patch-clamp studies on olfactory cilia revealed four different KCas, with di...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.01.079
更新日期:2005-03-14 00:00:00
abstract::In endocrine cells the regulated secretion of hormones, peptides, enzymes and neurotransmitters into the external medium occurs when mature secretory granules fuse with the plasma membrane. Secretory granules form at the trans-Golgi network (TGN) by envelopment of the dense-core aggregate of regulated secretory protei...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(91)80805-d
更新日期:1991-07-22 00:00:00
abstract::Investigations of the cleavage specificity of a cyclizing endoribonuclease of yeast show that it hydrolyzes Y-A bonds of single-stranded regions of RNA and that oligonucleotides are hydrolyzed poorly. The stringent specificity of the enzyme suggests that it may be useful for generating specific fragments of polyribonu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80899-7
更新日期:1986-09-15 00:00:00
abstract::Photo-oxidized low-density lipoprotein is cytotoxic to bovine aortic endothelial cells in a concentration-dependent manner. Total cell killing occurs at a concentration of 600 mumol/l lipid hydroperoxide (LOOH). Selenium deficiency enhances the toxicity of LOOH such that 300 mumol/l LOOH is cytotoxic. This toxicity is...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00178-c
更新日期:1995-03-20 00:00:00
abstract::In HCT116 colorectal cancer cells, HeLa cervical cancer cells and HuH-7 hepatoma cells, miR-223 is expressed at a low level. Through infection with lentivirus containing miR-223 precursor, miR-223 [corrected] was overexpressed in all these cells. Interestingly, the expression levels of FOXO1 mRNA and protein, and phos...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.02.050
更新日期:2012-04-05 00:00:00
abstract::Connexin 43 (Cx43) hemichannels may form open channels in the plasma membrane when exposed to specific stimuli, e.g. reduced extracellular concentration of divalent cations, and allow passage of fluorescent molecules and presumably a range of smaller physiologically relevant molecules. However, the permeability profil...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.01.036
更新日期:2014-04-17 00:00:00
abstract::In response to Ca(2+) signaling, cytosolic phospholipase A(2)alpha (cPLA(2)alpha) translocates from the cytosol to the perinuclear membrane, where downstream eicosanoid-synthetic enzymes, such as cyclooxygenase (COX), are localized. Although the spatiotemporal perinuclear colocalization of cPLA(2)alpha and COXs has be...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00596-9
更新日期:2003-07-10 00:00:00
abstract::Zinc metallopeptidases that contain the His-Glu-Xaa-Xaa-His (HEXXH) motif generally have a third ligand of the metal ion that may be either a Glu residue (in clan MA) or a His residue (in clan MB) (Rawlings and Barrett (1995) Methods Enzymol. 248, 183-228). Thimet oligopeptidase has not yet been assigned to either cla...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01032-1
更新日期:1998-09-11 00:00:00
abstract::Gene expression is controlled through a complex interplay among mRNAs, non-coding RNAs and RNA-binding proteins (RBPs), which all assemble along with other RNA-associated factors in dynamic and functional ribonucleoprotein complexes (RNPs). To date, our understanding of RBPs is largely limited to proteins with known o...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2014.07.039
更新日期:2014-10-16 00:00:00
abstract::We studied the molecular binding specificity of two rabbit polyclonal sera generated against phenolic glycolipid antigens namely PheG1 B and PheG1 B-3 from Mycobacterium bovis BCG. PheG1 B is the well-known mycoside B (2-O-Me-alpha-L-Rhap 1----aglycone), while PheG1 B-3 is a recently found glycolipid (alpha-L-Rhap-(1-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80469-w
更新日期:1992-05-25 00:00:00
abstract::Glutathione peroxidase 3 (GPX3) is an important member of antioxidant enzymes for reducing reactive oxygen species and maintaining the oxygen balance. Gpx3 mRNA is strongly expressed in decidual cells from days 5 to 8 of pregnancy. After pregnant mice are treated with GPX inhibitor for 3 days, pregnancy rate is signif...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.02.043
更新日期:2014-05-02 00:00:00
abstract::Diapause is an important overwintering strategy enabling Locusta migratoria to survive under stressed conditions. We identified a novel dual-specificity kinase gene that is differentially expressed between long and short day-treated L. migratoria. To determine its function on photoperiodic diapause induction, we clone...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13544
更新日期:2019-11-01 00:00:00
abstract::Using time-resolved low-temperature spectroscopy, we have examined whether or not bovine rhodopsin has a unique transducin-binding state, meta Ib, previously detected from chicken rhodopsin. Unlike chicken meta Ib, bovine meta Ib was detected only by detailed kinetics analysis of the bleaching process, but it was stab...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00216-6
更新日期:1998-03-20 00:00:00
abstract::14-3-3s predominantly form homo-/heterodimers that are in equilibrium with corresponding monomers. Dimer/monomer equilibrium depends on the nature and phosphorylation of Ser58 of certain 14-3-3 isoforms. The structure and properties of 14-3-3 dimers are well characterized, whereas 14-3-3 monomers are less investigated...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2012.10.048
更新日期:2012-12-14 00:00:00
abstract::The effect of platelet-derived growth factor (PDGF) on Rap1 expression was investigated in rat vascular smooth muscle cells (SMC). First, evidence for Rap1 proteins was shown by their: (i) detection in membranes using a specific anti-Rap1 antibody, (ii) typical shift in electrophoretic mobility as a consequence of red...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80492-3
更新日期:1994-04-04 00:00:00
abstract::The function of the domains of Trichoderma reesei endoglucanase I (EGI) has been studied. Truncated EGI proteins were expressed from the 3'-end deleted cDNAs in the yeast Saccharomyces cerevisiae under the control of the ADC1 expression cassette. EGI protein was detected by monoclonal antibody EI-2 and EGI activity as...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81100-m
更新日期:1991-10-07 00:00:00
abstract::Polyhydroxyalkanoate (PHA) synthases catalyze the polymerization reaction of the acyl moiety of hydroxyacyl-coenzyme A into polyester. The catalytic subunit PhaC of PHA synthase has the PhaC box sequence at the active site that is typically described as G-X-C-X-G-G (X is an arbitrary amino acid), and cysteine is an ac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13651
更新日期:2020-02-01 00:00:00
abstract::CD38, a type II transmembrane glycoprotein, behaves as a catalytically active transporter responsible for ectocellular generation of cyclic ADP-ribose (cADPR) from NAD+ and for subsequent influx of cADPR across membranes [Franco, L., Guida, L., Bruzzone, S., Zocchi, E., Usai, C. and De Flora, A. (1998) FASEB J. in pre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00929-6
更新日期:1998-08-21 00:00:00
abstract::Bovine insulin can be split into several fractions by gel filtration on Sephadex G50. These are, in order of decreasing molecular weight, a new unidentified substance, proinsulin, and a large fraction containing several substances. Ultracentrifugal examination of the proinsulin in acetic acid solution (pH 2.3) gave a ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(68)80122-x
更新日期:1968-12-01 00:00:00
abstract::Elongation factor Tu (EF-Tu) is the most abundant protein in prokaryotic cells. Its general function in protein biosynthesis is well established. It is a member of the large family of G-proteins, all of which bind guanosine phosphates (GDP or GTP) as cofactors. In its active GTP bound state EF-Tu binds aminoacylated t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01254-7
更新日期:1994-12-19 00:00:00
abstract::We have studied the pharmacological properties of genetically engineered human NK1 tachykinin receptors in which residues at the extracellular surface of the fourth transmembranal domain were substituted with the corresponding amino acids from the NK2 receptor. We show that substitution of G166C:Y167F in the human NK1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01236-2
更新日期:1997-10-27 00:00:00
abstract::RNA binding motif protein 5 (RBM5) is a candidate tumor suppressor gene. Recent studies showed that RBM5 functions as an alternative splicing regulator of apoptosis-related genes. Here, we identify DHX15 and PRP19, two spliceosome components, as novel RBM5-interacting partners. We then show that the G-patch domain of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.02.052
更新日期:2012-04-05 00:00:00
abstract::A short conserved sequence equivalent to the fifth conserved sequence region of alpha-amylases (173_LPDLD, Aspergillus oryzae alpha-amylase) comprising the calcium-ligand aspartate, Asp-175, was identified in the amino acid sequences of several members of the family of (alpha/beta)8-barrel glycosyl hydrolases. Despite...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01309-1
更新日期:1995-12-11 00:00:00
abstract::We compared oxidative DNA damage in strictly anaerobic Prevotella melaninogenica, aerotolerant anaerobic Bacteroides fragilis, and facultative anaerobic Salmonella typhimurium after exposure to O2 or H2O2. Using HPLC with electrochemical detection, we measured 8-hydroxydeoxyguanosine (8OHdG) as a damage marker. O2 ind...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00497-4
更新日期:1999-05-07 00:00:00
abstract::We have recorded (13)C nuclear magnetic resonance (NMR) spectra of [3-(13)C]Ala, [1-(13)C]Val-labeled pharaonis phoborhodopsin (ppR or sensory rhodopsin II) incorporated into egg PC (phosphatidylcholine) bilayer, by means of site-directed high-resolution solid-state NMR techniques. Seven (13)C NMR signals from transme...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00065-6
更新日期:2003-02-11 00:00:00