Abstract:
:Gene expression is controlled through a complex interplay among mRNAs, non-coding RNAs and RNA-binding proteins (RBPs), which all assemble along with other RNA-associated factors in dynamic and functional ribonucleoprotein complexes (RNPs). To date, our understanding of RBPs is largely limited to proteins with known or predicted RNA-binding domains. However, various methods have been recently developed to capture an RNA of interest and comprehensively identify its associated RBPs. In this review, we discuss the RNA-affinity purification methods followed by mass spectrometry analysis (AP-MS); RBP screening within protein libraries and computational methods that can be used to study the RNA-binding proteome (RBPome).
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Faoro C,Ataide SFdoi
10.1016/j.febslet.2014.07.039subject
Has Abstractpub_date
2014-10-16 00:00:00pages
3649-64issue
20eissn
0014-5793issn
1873-3468pii
S0014-5793(14)00616-4journal_volume
588pub_type
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