Biochemical characterization and subcellular localization of the sterol C-24(28) reductase, erg4p, from the yeast saccharomyces cerevisiae.

abstract：:Human preimplantation developmental studies are difficult to accomplish due to associated ethical and moral issues. Preimplantation cells are rare and exist only in transient cell states. From a single cell, it is very challenging to analyse the origination of the heterogeneity and complexity inherent to the human bod...

journal_title：FEBS letters

authors： Otte J,Wruck W,Adjaye J

更新日期：2017-08-01 00:00:00

abstract：:The lack of trehalose accumulation in most plant species has been partly attributed to the presence of an active trehalase. Although trehalose synthesis enzymes are thought to be cytosolic, and previous studies have indicated that trehalase activity is extracellular, the exact location of the enzyme has not yet been e...

journal_title：FEBS letters

authors： Frison M,Parrou JL,Guillaumot D,Masquelier D,François J,Chaumont F,Batoko H

更新日期：2007-08-21 00:00:00

abstract：:The purinergic receptor P2X(7) is part of a complex signaling mechanism participating in a variety of physiological and pathological processes. Depending on the activation scheme, P2X(7) receptors in vivo are non-selective cation channels or form large pores that can mediate apoptotic cell death. Expression of P2X(7)R...

journal_title：FEBS letters

authors： Locovei S,Scemes E,Qiu F,Spray DC,Dahl G

更新日期：2007-02-06 00:00:00

abstract：:Peptides corresponding to the known sequence of turkey erythrocyte beta 1-adrenergic receptor were synthesized and the effects on receptor-mediated cyclase activation were measured. Peptides corresponding to the first and second intracellular loops (T61-71 and T138-159) inhibited at micromolar concentrations the hormo...

journal_title：FEBS letters

authors： Palm D,Münch G,Dees C,Hekman M

更新日期：1989-08-28 00:00:00

abstract：:Golgin45 plays important roles in Golgi stack assembly and is known to bind both the Golgi stacking protein GRASP55 and Rab2 in the medial-Golgi cisternae. In this study, we sought to further characterize the cisternal adhesion complex using a proteomics approach. We report here that Acyl-CoA binding domain containing...

journal_title：FEBS letters

authors： Yue X,Bao M,Christiano R,Li S,Mei J,Zhu L,Mao F,Yue Q,Zhang P,Jing S,Rothman JE,Qian Y,Lee I

更新日期：2017-09-01 00:00:00

abstract：:A short conserved sequence equivalent to the fifth conserved sequence region of alpha-amylases (173_LPDLD, Aspergillus oryzae alpha-amylase) comprising the calcium-ligand aspartate, Asp-175, was identified in the amino acid sequences of several members of the family of (alpha/beta)8-barrel glycosyl hydrolases. Despite...

journal_title：FEBS letters

authors： Janecek S

更新日期：1995-12-11 00:00:00

abstract：:A low resolution solution structure of the IIA domain of the Bacillus subtilis phosphoenolpyruvate-sugar phosphotransferase system (PTS) glucose permease has been determined using 945 inter-residue and 724 intra-residue distance constraints derived from three-dimensional 15N and 13C edited NOESY spectra. A total of 15...

journal_title：FEBS letters

authors： Fairbrother WJ,Gippert GP,Reizer J,Saier MH Jr,Wright PE

更新日期：1992-01-20 00:00:00

abstract：:Cyclic ADP-ribose (cADPR) is a natural metabolite of beta-NAD+ with a potent Ca2+-mobilizing activity in different cell types, including T-lymphocytes. We investigated (i) whether stimulation of T-lymphocytes with different agonists affects the intracellular concentration of cADPR, and (ii) whether the lymphocyte anti...

journal_title：FEBS letters

authors： da Silva CP,Schweitzer K,Heyer P,Malavasi F,Mayr GW,Guse AH

更新日期：1998-11-20 00:00:00

abstract：:Na(+)/H(+) antiporters are ubiquitous membrane proteins and play an important role in cell homeostasis. We amplified a gene encoding a member of the monovalent cation:proton antiporter-2 (CPA2) family (TC 2.A.37) from the Thermus thermophilus genome and expressed it in Escherichia coli. The gene product was identified...

journal_title：FEBS letters

authors： Furrer EM,Ronchetti MF,Verrey F,Pos KM

更新日期：2007-02-06 00:00:00

abstract：:The Escherichia coli signal transduction protein PII, product of the glnB gene, was overproduced and purified. The predicted molecular weight of the protein based on the correct nucleotide sequence is 12,427 and is very close to the value 12,435 obtained by matrix-assisted laser desorption mass spectrometry. Hexagonal...

journal_title：FEBS letters

authors： Vasudevan SG,Gedye C,Dixon NE,Cheah E,Carr PD,Suffolk PM,Jeffrey PD,Ollis DL

更新日期：1994-01-17 00:00:00

abstract：:Endothelial monocyte-activating polypeptide II (EMAP II) is a proinflammatory cytokine and a chemoattractant for leukocytes. The mature cytokine is formed in apoptotic cells by cleavage of the precursor proEMAP II. Here we show that caspase-7 is capable of cleaving proEMAP II in vitro. A proEMAP II mutant, in which th...

journal_title：FEBS letters

authors： Behrensdorf HA,van de Craen M,Knies UE,Vandenabeele P,Clauss M

更新日期：2000-01-21 00:00:00

abstract：:Quantitative structure activity relationships (QSARs) for the conversion of nitrobenzimidazolones and nitrobenzimidazoles by rat liver DT-diaphorase (EC 1.6.99.2) are described. The parameter used for description of the QSARs is the energy of the lowest unoccupied molecular orbital (E(LUMO)) of the nitroaromatic compo...

journal_title：FEBS letters

authors： Anusevicius Z,Soffers AE,Cénas N,Sarlauskas J,Segura-Aguilar J,Rietjens IM

更新日期：1998-05-15 00:00:00

abstract：:Large amounts of refined sequence material in the form of predicted, curated and annotated genes and expressed sequences tags (ESTs) have recently been added to the NCBI databases. We matched the transcript-sequences of RefSeq, Ensembl and dbEST in an attempt to provide an updated overview of how many unique human gen...

journal_title：FEBS letters

authors： Larsson TP,Murray CG,Hill T,Fredriksson R,Schiöth HB

更新日期：2005-01-31 00:00:00

abstract：:Vpu is an 81 amino acid protein encoded by HIV-1. Its role is to amplify viral release by two mechanisms: (i) docking to CD4 with the consequence of targeting CD4 for ubiquitine-mediated degradation, and (ii) formation of ion channels to enhance particle release. The intensive research on its in vivo function, combine...

journal_title：FEBS letters

authors： Fischer WB

更新日期：2003-09-18 00:00:00

abstract：:Challenge of Rhodobacter capsulatus cells with the superoxide propagator methyl viologen resulted in the induction of a diaphorase activity identified as a member of the ferredoxin (flavodoxin)-(reduced) nicotinamide adenine dinucleotide phosphate (NADP(H)) reductase (FPR) family by N-terminal sequencing. The gene cod...

journal_title：FEBS letters

authors： Bittel C,Tabares LC,Armesto M,Carrillo N,Cortez N

更新日期：2003-10-23 00:00:00

abstract：:Lactate dehydrogenase (LDH) and yeast alcohol dehydrogenase ( YADH ) are inactivated when attacked by hydroxy free radicals (OH). Organic molecules with a high rate constant of reaction with OH such as ascorbate or urate can compete with the enzymes for these strongly oxidising radicals. However, although 10(-3)M asco...

journal_title：FEBS letters

authors： Kittridge KJ,Willson RL

更新日期：1984-05-07 00:00:00

abstract：:Phenylalanine ammonia-lyase immobilized within semipermeable microcapsules has an assayed enzyme activity which is 20% +/- 4% of the enzyme in free solution. The Km for the immobilized enzyme remained the same as that of the free enzyme. The pH optimum also remained unchanged at pH 8.5 +/- 1.0. At the lower pH range, ...

journal_title：FEBS letters

authors： Bourget L,Chang TM

更新日期：1985-01-21 00:00:00

abstract：:A synthetic peptide, KKKKRFSFKKSFKLSGFSFKK, containing the phosphorylation sites of the acidic 80-87 kDa protein kinase C substrate was used to identify phosphopeptides in enzyme digests of this protein from mouse fibroblast C3H/10T1/2 cells. Stimulation of phosphorylation occurred, in vivo, with TPA at Ser7, Ser11 an...

journal_title：FEBS letters

authors： Amess B,Manjarrez-Hernandez HA,Howell SA,Learmonth M,Aitken A

更新日期：1992-02-10 00:00:00

abstract：:To investigate interactions between the polychlorinated biphenyl-binding protein uteroglobin and phospholipids, we used a liposome-pelletting assay. PCB-BP/uteroglobin bound to liposomes made from negatively charged phospholipids (PtdSer and PtdIns) in the presence of 5 mM calcium. No binding to liposomes made from ph...

journal_title：FEBS letters

authors： Nord M,Gustafsson JA,Lund J

更新日期：1995-11-06 00:00:00

abstract：:Apoptosis is a physiological process for active cell removal. One of its hallmarks is an increased cytosolic Ca2+ content. Several genes involved in apoptosis control have been identified, but their mode of action is not understood in detail. Apoptosis may relate to oncogenesis, in that some malignant tumors may grow ...

journal_title：FEBS letters

authors： Richter C

更新日期：1993-06-28 00:00:00

abstract：:The cytosolic lipid peroxidation product 4-hydroxynonenal (HNE) is rapidly metabolized in mitochondria isolated from rat kidney cortex. About 80% of HNE was degraded within 3 min of incubation. Main products of HNE which were identified in mitochondria were the hydroxynonenoic acid, the 1,4-dihydroxynonene and the glu...

journal_title：FEBS letters

authors： Ullrich O,Grune T,Henke W,Esterbauer H,Siems WG

更新日期：1994-09-19 00:00:00

abstract：:Gp130 is the common signal transducing receptor subunit of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor and cardiotrophin-1. IL-6 and IL-11 induce gp130 homodimerization whereas the others lead to the formation of heterodimers with LIFR or OSMR. Binding e...

journal_title：FEBS letters

authors： Timmermann A,Pflanz S,Grötzinger J,Küster A,Kurth I,Pitard V,Heinrich PC,Müller-Newen G

更新日期：2000-02-25 00:00:00

abstract：:Pore-forming toxins, such as colicin A, are water-soluble proteins that insert into lipid bilayers. The water-soluble structure of Colicin A is known at a high resolution and this review describes the kinetic and structural steps involved in its soluble-to-membrane bound transformation. ...

journal_title：FEBS letters

authors： Lakey JH,González-Mañas JM,van der Goot FG,Pattus F

更新日期：1992-07-27 00:00:00

abstract：:The binding of protein HU from Escherichia coli to nucleic acids was investigated by affinity chromatography under various conditions, by a nitrocellulose retention assay and by isopycnic centrifugations in metrizamide gradients. The results indicate that HU has a preference for binding to RNA and single-stranded DNA ...

journal_title：FEBS letters

authors： Holck A,Kleppe K

更新日期：1985-06-03 00:00:00

abstract：:In bovine heart mitochondria a protein of M(r) 18 kDa, phosphorylated by mtPKA, is associated to the NADH-ubiquinone oxidoreductase in the inner membrane and is present in purified preparation of this complex. The 18 kDa phosphoprotein has now been isolated and sequenced. It is identified as the 18 kDa (IP) AQDQ subun...

journal_title：FEBS letters

authors： Papa S,Sardanelli AM,Cocco T,Speranza F,Scacco SC,Technikova-Dobrova Z

更新日期：1996-02-05 00:00:00

abstract：:Saccharomyces bayanus var. uvarum investigated here is the species closest to Saccharomyces cerevisiae. Random sequence tags (RSTs) allowed us to identify homologues to 2789 open reading frames (ORFs) in S. cerevisiae, ORFs duplicated in S. uvarum but not in S. cerevisiae, centromeres, tRNAs, homologues of Ty1/2 and T...

journal_title：FEBS letters

authors： Bon E,Neuvéglise C,Casaregola S,Artiguenave F,Wincker P,Aigle M,Durrens P

更新日期：2000-12-22 00:00:00

abstract：:A single protein from human leukocytes possesses both 5-lipoxygenase and leukotriene A4 (LTA4) synthase activities. It has been reported that LTA4 production is more efficient when the enzyme utilizes arachidonic acid, than when 5-HPETE is exogenously supplied as substrate. In the present study, human leukocyte homoge...

journal_title：FEBS letters

authors： Puustinen T,Scheffer MM,Samuelsson B

更新日期：1987-06-15 00:00:00

abstract：:Using antibodies recognizing the phosphorylation state of specific sites, phosphorylation states of tau were monitored in fetal rat primary cultured neurons. When cultured neurons were treated with okadaic acid (OA) or calyculin A (CalA) at concentrations sufficient to inhibit protein phosphatase 2A (PP2A), phosphoryl...

journal_title：FEBS letters

authors： Saito T,Ishiguro K,Uchida T,Miyamoto E,Kishimoto T,Hisanaga S

更新日期：1995-12-04 00:00:00

abstract：:A functional rat heart Na(+)-Ca2+ exchanger gene has been obtained by splicing and ligating two partially overlapping clones isolated from a rat heart lambda ZAP cDNA library. The deduced primary structure of the protein encoded by the open reading frame corresponds to 971 amino acids, that can be organized into 12 tr...

journal_title：FEBS letters

authors： Low W,Kasir J,Rahamimoff H

更新日期：1993-01-18 00:00:00

abstract：:A single chain ribosome-inactivating protein with RNA N-glycosidase activity, here named Dianthin 29, was isolated from leaves of Dianthus barbatus L. Incubation of intact Escherichia coli ribosomes with Dianthin 29 and subsequent aniline treatment of the isolated rRNA releases a rRNA fragment of 243 nucleotides from ...

journal_title：FEBS letters

authors： Prestle J,Hornung E,Schönfelder M,Mundry KW

更新日期：1992-02-10 00:00:00