The nuclear-encoded 18 kDa (IP) AQDQ subunit of bovine heart complex I is phosphorylated by the mitochondrial cAMP-dependent protein kinase.


:In bovine heart mitochondria a protein of M(r) 18 kDa, phosphorylated by mtPKA, is associated to the NADH-ubiquinone oxidoreductase in the inner membrane and is present in purified preparation of this complex. The 18 kDa phosphoprotein has now been isolated and sequenced. It is identified as the 18 kDa (IP) AQDQ subunit of complex I, a protein of 133 amino acids with a phosphorylation consensus site RVS at position 129-131.




FEBS letters


Papa S,Sardanelli AM,Cocco T,Speranza F,Scacco SC,Technikova-Dobrova Z




Has Abstract


1996-02-05 00:00:00














  • Stabilization of a semiquinone radical at the high-affinity quinone-binding site (QH) of the Escherichia coli bo-type ubiquinol oxidase.

    abstract::Reaction of ubiquinone in the high-affinity quinone-binding site (QH) in bo-type ubiquinol oxidase from Escherichia coli was revealed by EPR and optical studies. In the QH site, ubiquinol was shown to be oxidized to ubisemiquinone and to ubiquinone, while no semiquinone signal was detected in the oxidase isolated from...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sato-Watanabe M,Itoh S,Mogi T,Matsuura K,Miyoshi H,Anraku Y

    更新日期:1995-10-30 00:00:00

  • Molecular cloning of a cDNA that encodes a serine protease with chymotryptic and collagenolytic activities in the hepatopancreas of the shrimp Penaeus vanameii (Crustacea, Decapoda).

    abstract::Two clones were isolated by screening a shrimp hepatopancreas cDNA library with a DNA fragment obtained by PCR amplification using two oligonucleotides based on the partial protein sequence of Penaeus vanameii chymotrypsin purified earlier. One of these clones, PVC 7 contains a complete cDNA coding for a serine protea...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sellos D,Van Wormhoudt A

    更新日期:1992-09-14 00:00:00

  • Requirements for a conservative protein translocation pathway in chloroplasts.

    abstract::The chloroplast inner envelope translocon subunit Tic110 is imported via a soluble stromal translocation intermediate. In this study an in-organellar import system is established which allows for an accumulation of this intermediate in order to analyze its requirements for reexport. All results demonstrate that the re...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vojta L,Soll J,Bölter B

    更新日期:2007-06-12 00:00:00

  • Identification of the cardiac ryanodine receptor channel in membrane blebs of sarcoplasmic reticulum.

    abstract::Blebs of the sarcoplasmic reticulum (SR) membrane of heart muscle cells were generated after saponin perforation of the plasma membrane followed by complete hypercontraction of the cell. Although characteristic proteins of the plasma membrane, namely the beta1-adrenoreceptor and Galphai, were stained by monoclonal ant...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Böhle T,Brandt MC,Henn N,Schmidt A,Bloch W,Beuckelmann DJ

    更新日期:2001-09-21 00:00:00

  • Does calmitine, a protein specific for the mitochondrial matrix of skeletal muscle, play a key role in mitochondrial function?

    abstract::The effect of the myotoxic drug chlorpromazine was studied in vitro on proteins of sarcoplasmic reticulum and mitochondrial matrix of skeletal muscle in the normal mouse. Our results indicate that the drug is specific for calcium-binding proteins (calcium ATPase, calsequestrin and calmitine). Its proteolytic effect on...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lucas-Heron B,Le Ray B,Schmitt N

    更新日期:1995-11-06 00:00:00

  • Muscle glycolipids in inherited muscular dystrophy of chickens.

    abstract::Gangliosides and neutral glycolipids of muscles from normal and dystrophic chickens were studied. Total glycolipid content of the degenerating muscles was higher than the normal muscles. In addition, the myopathic muscles contained a ganglioside which was absent in the unaffected muscles from normal and dystrophic chi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kundu SK,Misra LK,Luthra MG

    更新日期:1982-12-27 00:00:00

  • The yeast Golgi apparatus: insights and mysteries.

    abstract::The Golgi apparatus is known to modify and sort newly synthesized secretory proteins. However, fundamental mysteries remain about the structure, operation, and dynamics of this organelle. Important insights have emerged from studying the Golgi in yeasts. For example, yeasts have provided direct evidence for Golgi cist...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Papanikou E,Glick BS

    更新日期:2009-12-03 00:00:00

  • RNA/DNA hybrid duplexes with identical nearest-neighbor base-pairs have identical stability.

    abstract::Energetic behaviors of eight pairs of RNA/DNA hybrid duplexes with identical nearest neighbors have been investigated by UV melting analysis. In the pairs with identical nearest-neighbor pairs, the melting curve traces at the same strand concentration were very similar. The average difference in stabilization energy o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sugimoto N,Katoh M,Nakano S,Ohmichi T,Sasaki M

    更新日期:1994-10-31 00:00:00

  • SRC transcriptional activation in a subset of human colon cancer cell lines.

    abstract::Human SRC encodes the non-receptor tyrosine kinase pp60(c-Src), which is activated in many human colon cancer cell lines (HCCLs) and tumors. We found that both c-Src protein and mRNA levels were elevated in a subset of HCCLs. Increased c-Src mRNA and protein levels correlated strongly with increased c-Src kinase activ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dehm S,Senger MA,Bonham K

    更新日期:2001-01-05 00:00:00

  • Cell fate determination, neuronal maintenance and disease state: The emerging role of transcription factors Lmx1a and Lmx1b.

    abstract::LIM-homeodomain (LIM-HD) proteins are evolutionary conserved developmental transcription factors. LIM-HD Lmx1a and Lmx1b orchestrate complex temporal and spatial gene expression of the dopaminergic pathway, and evidence shows they are also involved in adult neuronal homeostasis. In this review, the multiple roles play...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Doucet-Beaupré H,Ang SL,Lévesque M

    更新日期:2015-12-21 00:00:00

  • The p18 component of the multisynthetase complex shares a protein motif with the beta and gamma subunits of eukaryotic elongation factor 1.

    abstract::In higher eukaryotes, nine aminoacyl-tRNA synthetases form a multienzyme complex also comprising the three auxiliary proteins p18, p38 and p43, of apparent molecular masses of 18, 38 and 43 kDa. The function of these proteins, invariably found associated to the synthetase components of the complex, is unknown. In orde...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Quevillon S,Mirande M

    更新日期:1996-10-14 00:00:00

  • The evolution of BIR domain and its containing proteins.

    abstract::BIR domain and its containing proteins play critical roles in cell apoptosis and cell division. Here several lines of novelty were revealed based on a comprehensive evolutionary analysis of BIR domains in 11 representative organisms. First, the type II BIR domains in Survivin and Bruce showed more conservation compare...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cao L,Wang Z,Yang X,Xie L,Yu L

    更新日期:2008-11-12 00:00:00

  • Purification of two astroglial growth factors from bovine brain.

    abstract::The astroglial growth factor (AGF), which induces a characteristic morphological change in cultured rat astroglial cells and stimulates their proliferation, was purified to homogeneity from bovine brain. Two different methods were used, the second one including heparin-Sepharose affinity chromatography. AGF is actuall...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pettmann B,Weibel M,Sensenbrenner M,Labourdette G

    更新日期:1985-09-09 00:00:00

  • Two types of linkage between codon usage and gene-expression levels.

    abstract::The relation between codon usage and gene-expression levels is an intensively investigated and discussed topic in the field of molecular evolution. We statistically analyzed 25 Escherichia coli gene sequences by a new classification of synonymous codons and found that (i) there are two distinct types of linkage betwee...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nakamura T,Suyama A,Wada A

    更新日期:1991-09-02 00:00:00

  • Nuclear functions in space and time: gene expression in a dynamic, constrained environment.

    abstract::All eukaryotic cells enclose their genome within a dedicated, membrane-bound organelle termed the nucleus, which functions to partition gene transcription from sites of protein translation in the cytoplasm. Despite a great deal of research effort, basic questions about chromosome structure and gene expression mechanis...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Trinkle-Mulcahy L,Lamond AI

    更新日期:2008-06-18 00:00:00

  • Human peripheral blood lymphocytes express D5 dopamine receptor gene and transcribe the two pseudogenes.

    abstract::Sequential reverse transcription and polymerase chain reaction (RT-PCR) of the mRNA were used to investigate the expression of dopamine receptors in human peripheral blood lymphocytes. The RT-PCR products contained three types of sequences, each corresponding to those of the D5 dopamine receptor gene and the two relat...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Takahashi N,Nagai Y,Ueno S,Saeki Y,Yanagihara T

    更新日期:1992-12-07 00:00:00

  • IgG immunoglobulins induce activation of the sphingomyelin cycle in HL-60 cells.

    abstract::In HL-60 cells signal transduction via sphingomyelin hydrolysis (sphingomyelin cycle) is induced by binding of tumor necrosis factor alpha (TNFalpha) to cell surface TNFalpha receptor. We found that IgG immunoglobulins activate sphingomyelin hydrolysis in plasma membrane of HL-60 cells, with kinetics similar to that o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Glick D,Barenholz Y

    更新日期:1996-10-07 00:00:00

  • Fluorescence ratiometric detection of ligand-induced receptor internalization using extracellular coiled-coil tag-probe labeling.

    abstract::We report a new method for the detection of ligand-induced receptor internalization by fluorescence ratiometric imaging of pH in endosomes in combination with a recently developed posttranslational labeling system based on the formation of a heterodimeric coiled-coil structure. The N-terminus of the β2-adrenergic rece...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yano Y,Matsuzaki K

    更新日期:2011-07-21 00:00:00

  • Pentacoordination of the heme iron of Arthromyces ramosus peroxidase shown by a 1.8 A resolution crystallographic study at pH 4.5.

    abstract::In the presence of ammonium sulfate the absorption spectra of a peroxidase from the fungus Arthromyces ramosus (ARP) showed that the low-spin component increased as the pH increased from 6.0 to 9.0, whereas in its absence ARP remained in the high-spin state in the pH range investigated. The crystal structure of ARP at...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kunishima N,Amada F,Fukuyama K,Kawamoto M,Matsunaga T,Matsubara H

    更新日期:1996-01-15 00:00:00

  • Effects of hydrogen/deuterium exchange on photosynthetic water cleavage in PS II core complexes from spinach.

    abstract::H/D isotope exchange effects on P680+. reduction by Yz and electron abstraction from the water oxidizing complex (WOC) in redox state S3 by YZOX were analyzed in PS II core complexes from spinach by measurements of laser flash induced absorption changes at 820 nm and 355 nm. The results obtained reveal: (1) the rate o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Karge M,Irrgang KD,Sellin S,Feinäugle R,Liu B,Eckert HJ,Eichler HJ,Renger G

    更新日期:1996-01-08 00:00:00

  • Photoreceptors and olfactory cells express the same retinal guanylyl cyclase isoform in medaka: visualization by promoter transgenics.

    abstract::We examined the spatial expression patterns of two orphan receptor guanylyl cyclase genes OlGC4 and OlGC5 during embryogenesis of medaka and characterized the 5' flanking region required for tissue-specific expression of OlGC4 by introducing promoter-GFP fusion constructs into medaka embryos. Expression of OlGC5 is co...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kusakabe T,Suzuki N

    更新日期:2000-10-20 00:00:00

  • Expression of a chimeric, cGMP-sensitive regulatory subunit of the cAMP-dependent protein kinase type I alpha.

    abstract::To study the fluctuations of cGMP in living cells through changes of energy transfer of dissociable fluorescence labeled subunits, we constructed a cGMP-sensitive probe by combining the N-terminus of the type I regulatory subunit of cAMP-dependent protein kinase (PKA) with the cGMP binding sites of cGMP-dependent prot...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wild N,Herberg FW,Hofmann F,Dostmann WR

    更新日期:1995-11-06 00:00:00

  • Kinetics of the interaction of endotoxin with polymyxin B and its analogs: a surface plasmon resonance analysis.

    abstract::Lipopolysaccharide, the invariant structural component of Gram-negative bacteria, when present in minute amounts in the circulation in humans elicits 'endotoxic shock' syndrome, which is fatal in 60% of the cases. Polymyxin B (PMB), a cyclic cationic peptide, neutralizes the endotoxin, but also induces many harmful si...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Thomas CJ,Surolia A

    更新日期:1999-02-26 00:00:00

  • Inhibition of glycosphingolipid synthesis induces p34cdc2 activation in Xenopus oocyte.

    abstract::In Xenopus prophase-blocked oocytes, it is assumed that progesterone interacts with the plasma membrane to initiate a signalling cascade that ultimately leads to MPF activation. Progesterone regulates negatively the cAMP pathway through an inhibition of adenylate cyclase. However, the mechanisms linking the initial ac...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: De Smedt V,Rime H,Jessus C,Ozon R

    更新日期:1995-11-20 00:00:00

  • A human synaptic vesicle monoamine transporter cDNA predicts posttranslational modifications, reveals chromosome 10 gene localization and identifies TaqI RFLPs.

    abstract::A human vesicular monoamine transporter cDNA has been identified by screening a human brainstem library using sequences from the rat brain synaptic vesicle monoamine transporter (SVMT) [(1992) Cell 70, 539-551; (1992) Proc. Natl. Acad. Sci. USA 89, 10993-10997]. The hSVMT shares 92% amino acid identity with the rat se...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Surratt CK,Persico AM,Yang XD,Edgar SR,Bird GS,Hawkins AL,Griffin CA,Li X,Jabs EW,Uhl GR

    更新日期:1993-03-08 00:00:00

  • Alternative splicing of the human Shaker K+ channel beta 1 gene and functional expression of the beta 2 gene product.

    abstract::Mammalian voltage-activated Shaker K+ channels associate with at least three cytoplasmic proteins: Kv beta 1, Kv beta 2 and Kv beta 3. These beta subunits contain variable N-termini, which can modulate the inactivation of Shaker alpha subunits, but are homologous throughout an aldo-keto reductase core. Human and ferre...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: McCormack K,McCormack T,Tanouye M,Rudy B,Stühmer W

    更新日期:1995-08-14 00:00:00

  • The detection of natural opal suppressor seryl-tRNA in Escherichia coli by the dot blot hybridization and phosphorylation by a tRNA kinase [corrected] .

    abstract::It was believed that there was no natural suppressor tRNA in Escherichia coli, however, it has been suggested that selC, relating to the synthesis of formate dehydrogenase of a selenoprotein [(1988) Nature 331, 723-725], codes for tRNA, even though the presence of tRNA has not yet been demonstrated. We detected the pr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mizutani T,Maruyama N,Hitaka T,Sukenaga Y

    更新日期:1989-04-24 00:00:00

  • Antithrombin Budapest 3. An antithrombin variant with reduced heparin affinity resulting from the substitution L99F.

    abstract::The molecular basis and functional properties of a variant antithrombin (AT) protein. AT Budapest 3, were studied. A single base substitution was identified in codon 99, CTC----TTC, altering the normal leucine to phenylalanine. The proband presented with a history of venous thrombotic disease and was found to be homoz...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Olds RJ,Lane DA,Boisclair M,Sas G,Bock SC,Thein SL

    更新日期:1992-04-06 00:00:00

  • Molecular mechanism and physiological role of pexophagy.

    abstract::Pexophagy is a selective autophagy process wherein damaged and/or superfluous peroxisomes undergo vacuolar degradation. In methylotropic yeasts, where pexophagy has been studied most extensively, this process occurs by either micro- or macropexophagy: processes analogous to micro- and macroautophagy. Recent studies ha...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Manjithaya R,Nazarko TY,Farré JC,Subramani S

    更新日期:2010-04-02 00:00:00

  • Somatostatinergic phenotype markers in the human neuroblastoma cell-line LA-N-2.

    abstract::We have characterized somatostatinergic phenotype markers of the human neuroblastoma, LA-N-2. A single mRNA-transcript (approximately 850bp) and two cellular somatostatin immunoreactivity forms, a high molecular weight form (M(r) 15,000) and a fragment corresponding to somatostatin-28 was found, while the somatostatin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nilsson L,Bergström L,Meyersson G,Påhlman S,Winblad B,Folkesson R

    更新日期:1995-09-18 00:00:00