Abstract:
:A functional rat heart Na(+)-Ca2+ exchanger gene has been obtained by splicing and ligating two partially overlapping clones isolated from a rat heart lambda ZAP cDNA library. The deduced primary structure of the protein encoded by the open reading frame corresponds to 971 amino acids, that can be organized into 12 transmembrane helices. The cloned gene was functionally expressed in HeLa cells. Maximal expression was detected 18 h after transfection, after which transport activity rapidly declined. The electrogenic properties of the cloned transporter were demonstrated following reconstitution of the expressed exchanger protein into a tightly sealed phospholipid membrane.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Low W,Kasir J,Rahamimoff Hdoi
10.1016/0014-5793(93)81737-ksubject
Has Abstractpub_date
1993-01-18 00:00:00pages
63-7issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(93)81737-Kjournal_volume
316pub_type
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