Abstract:
:The polymorphism at the multitude of loci adjacent to human endogenous retrovirus long terminal repeats (LTRs) was analyzed by a technique for whole genome differential display based on the PCR suppression effect that provides selective amplification and display of genomic sequences flanking interspersed repeated elements. This strategy is simple, target-specific, requires a small amount of DNA and provides reproducible and highly informative data. The average frequency of polymorphism observed in the vicinity of the LTR insertion sites was found to be about 12%. The high incidence of polymorphism within the LTR flanks together with the frequent location of LTRs near genes makes the LTR loci a useful source of polymorphic markers for gene mapping.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lavrentieva I,Broude NE,Lebedev Y,Gottesman II,Lukyanov SA,Smith CL,Sverdlov EDdoi
10.1016/s0014-5793(99)00004-6keywords:
subject
Has Abstractpub_date
1999-01-29 00:00:00pages
341-7issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00004-6journal_volume
443pub_type
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