Abstract:
:Acceptor stem variants of tRNA(fMet) (Escherichia coli) have been characterized by nuclear magnetic resonance. The wild type contains a C1-A72 mismatch pair which is crucial for its biological function. For comparison, the mismatch was replaced by regular pairs U1-A72 and C1-G72. Further variants contain an altered discriminator base, G73, or a G1-C72/U73 combination. The stems of variants U1-A72/A73 and C1-G72/A73 have A-RNA geometry, which extends essentially to the single-strand terminus. C1-A72/G73 variant and wild type are structurally almost identical. C1 and A72 adopt peculiar conformations with C1 being largely destacked with respect to G2, while A73 stacks upon C1. The unique arrangement of the mismatch causes a distinctly different orientation of the single-strand terminus compared to variants with regular 1-72 base pairs, and to formyltransferase-complexed tRNA(fMet).
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Zuleeg T,Vogtherr M,Schübel H,Limmer Sdoi
10.1016/s0014-5793(00)01459-9keywords:
subject
Has Abstractpub_date
2000-04-28 00:00:00pages
247-53issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01459-9journal_volume
472pub_type
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