Interaction of a human blood group Sd(a-) Tamm-Horsfall glycoprotein with applied lectins.

abstract：:The 4-chlorobenzoyl-CoA dehalogenase catalyzes the hydrolytic dechlorination of 4-chlorobenzoyl-CoA via a two-step mechanism, namely nucleophilic aromatic substitution and ester hydrolysis. The mutation of an active-site Histidine residue has been shown to reduce the catalytic activity in both the substitution and sub...

journal_title：FEBS letters

authors： Xu D,Guo H

更新日期：2005-08-15 00:00:00

abstract：:Incubation of human thrombocytes in the presence of [3H]palmitic acid leads to incorporation of this fatty acid into the alpha subunit of Gs as described [Linder et al., Proc. Natl. Acad. Sci. USA 90 (1993) 3675-3679; Degtyarev et al., Biochemistry 32 (1993) 8057-8061] but also into the catalyst of adenylyl cyclase wh...

journal_title：FEBS letters

authors： Mollner S,Beck K,Pfeuffer T

更新日期：1995-09-11 00:00:00

abstract：:The alternative nitrogenase of Rhodobacter capsulatus, isolated from a nifHDK deletion mutant, has been purified to near homogeneity and identified as an 'iron only' nitrogenase. The dithionite-reduced component 1 ('FeFe protein') of this enzyme showed an EPR spectrum consisting of two components: a minor S = 1/2 sign...

journal_title：FEBS letters

authors： Müller A,Schneider K,Knüttel K,Hagen WR

更新日期：1992-05-25 00:00:00

abstract：:Replacements of Asp-295 by Asn (D295N) and Glu (D295E) decreased the catalytic center activity of Leuconostoc mesenteroides sucrose phosphorylase to about 0.01% of the wild-type level (k(cat)=200s(-1)). Glucosylation and deglucosylation steps of D295N were affected uniformly, approximately 10(4.3)-fold, and independen...

journal_title：FEBS letters

authors： Mueller M,Nidetzky B

更新日期：2007-04-03 00:00:00

abstract：:The unfolded protein response (UPR)--the endoplasmic reticulum stress response--is found in various pathologies including ischemia-reperfusion injury (IRI). However, its role during IRI is still unclear. Here, by combining two different bioinformatical methods--a method based on ordinary differential equations (Time S...

journal_title：FEBS letters

authors： Le Pape S,Dimitrova E,Hannaert P,Konovalov A,Volmer R,Ron D,Thuillier R,Hauet T

更新日期：2014-08-25 00:00:00

abstract：:An essential difference between eukaryotic ferritins and bacterioferritins is that the latter contain naturally, in vivo haem as Fe-protoporphyrin IX. This haem is located in a hydrophobic pocket along the 2-fold symmetry axes and is liganded by two Met 52. However, in in vivo studies, a cofactor has been isolated in ...

journal_title：FEBS letters

authors： de Val N,Herschbach H,Potier N,Dorsselaer AV,Crichton RR

更新日期：2006-11-13 00:00:00

abstract：:We present a new water selective pulse sequence allowing rapid determination of exchange rates of labile protons on the millisecond time scale. Using diffusion measurements, exchange rates of resolved protons can be determined without prior knowledge of relaxation parameters in a short overnight experiment. The use of...

journal_title：FEBS letters

authors： Böckmann A,Guittet E

更新日期：1997-11-24 00:00:00

abstract：:Circadian clocks are endogenous timing systems orchestrating the daily regulation of a huge variety of physiological, metabolic and behavioral processes. These clocks are important for health - in mammals, their disruption leads to a diverse number of pathologies. While genetic and biochemical approaches largely uncov...

journal_title：FEBS letters

authors： Wallach T,Kramer A

更新日期：2015-06-22 00:00:00

abstract：:An efficient method is presented for the production of intact mammalian prion proteins and partial sequences thereof. As an illustration we describe the production of polypeptides comprising residues 23-231, 81-231, 90-231 and 121-231 of the human prion protein (hPrP). Polypeptides were expressed as histidine tail fus...

journal_title：FEBS letters

authors： Zahn R,von Schroetter C,Wüthrich K

更新日期：1997-11-17 00:00:00

abstract：:We investigated the signaling pathways participating in hyperosmotic regulation of the human taurine transporter (TAUT) by using specific inhibitors of various intracellular signaling molecules. Among them, the specific inhibitor of calcium/calmodulin-dependent protein kinase II (Ca(2+)/CaM kinase II) completely repre...

journal_title：FEBS letters

authors： Satsu H,Manabe M,Shimizu M

更新日期：2004-07-02 00:00:00

abstract：:Thimet oligopeptidase (EC 3.4.24.15; TOP) is a Zn(II) endopeptidase implicated in physiological regulation of processes involving neuropeptides. The present study clarifies the active site structure and mechanism of catalysis of TOP. The enzyme exhibited a bell-shaped pH dependence of activity having an acidic limb du...

journal_title：FEBS letters

authors： Sigman JA,Edwards SR,Pabon A,Glucksman MJ,Wolfson AJ

更新日期：2003-06-19 00:00:00

abstract：:Compared with algal and cyanobacterial cytochrome c(6), cytochrome c(6A) from higher plants contains an additional loop of 12 amino acid residues. We have determined the first crystal structure of cytochrome c(6A) from Arabidopsis thaliana at 1.5 Angstrom resolution in order to help elucidate its function. The overall...

journal_title：FEBS letters

authors： Chida H,Yokoyama T,Kawai F,Nakazawa A,Akazaki H,Takayama Y,Hirano T,Suruga K,Satoh T,Yamada S,Kawachi R,Unzai S,Nishio T,Park SY,Oku T

更新日期：2006-06-26 00:00:00

abstract：:Endothelial dysfunction plays a major role in the pathogenesis of atherosclerosis. Pro-inflammatory cytokines such as interleukin-1 beta and tumour necrosis factor alpha activate endothelial cells changing their resting phenotype to become pro-adhesive, pro-thrombotic and pro-atherogenic. Phage display in vivo biopann...

journal_title：FEBS letters

authors： Houston P,Goodman J,Lewis A,Campbell CJ,Braddock M

更新日期：2001-03-09 00:00:00

abstract：:The Rad51 gene of Saccharomyces cerevisiae is required for genetic recombination and recombinational repair of DNA strand breaks. In higher eukaryotes Rad51 is essential for embryonic development, and is involved in cell proliferation and DNA repair. Here we show that human Rad51 (HsRad51) is proteolytically cleaved d...

journal_title：FEBS letters

authors： Flygare J,Armstrong RC,Wennborg A,Orsan S,Hellgren D

更新日期：1998-05-08 00:00:00

abstract：:Smad ubiquitination regulatory factor 2 (Smurf2), a ubiquitin ligase for Smads, plays critical roles in the regulation of transforming growth factor-beta (TGF-beta)-Smad signaling via ubiquitin-dependent degradation of Smad2 and Smad7. We found that TGF-beta stimulates Smurf2 expression. TGF-beta activated the Smurf2 ...

journal_title：FEBS letters

authors： Ohashi N,Yamamoto T,Uchida C,Togawa A,Fukasawa H,Fujigaki Y,Suzuki S,Kitagawa K,Hattori T,Oda T,Hayashi H,Hishida A,Kitagawa M

更新日期：2005-05-09 00:00:00

abstract：:The bchH gene of Rhodobacter capsulatus has been cloned into an expression strain of Escherichia coli. Following induction of expression of the BchH protein, it was found that the E. coli strain also accumulated porphyrins with the fluorescence properties of protoporphyrin and zinc protoporphyrin. It was also found th...

journal_title：FEBS letters

authors： Hinchigeri SB,Hundle B,Richards WR

更新日期：1997-05-05 00:00:00

abstract：:Phospholipase A2 has been purified from the venom of Horned viper (Cerastes cerastes) by gel permeation chromatography followed by reverse-phase HPLC. The primary structure was established by sequence analysis of the intact protein and its enzymic peptides. The structure has 120 residues, properties like other group I...

journal_title：FEBS letters

authors： Siddiqi AR,Shafqat J,Zaidi ZH,Jörnvall H

更新日期：1991-01-14 00:00:00

abstract：:We have recorded (13)C nuclear magnetic resonance (NMR) spectra of [3-(13)C]Ala, [1-(13)C]Val-labeled pharaonis phoborhodopsin (ppR or sensory rhodopsin II) incorporated into egg PC (phosphatidylcholine) bilayer, by means of site-directed high-resolution solid-state NMR techniques. Seven (13)C NMR signals from transme...

journal_title：FEBS letters

authors： Arakawa T,Shimono K,Yamaguchi S,Tuzi S,Sudo Y,Kamo N,Saitô H

更新日期：2003-02-11 00:00:00

abstract：:We tested the truncated 7-37 glucagon-like peptide 1 (TGLP-1), a naturally occurring porcine intestinal peptide, and other members of the glucagon family, including pancreatic glucagon (G-29), GLP-1 and GLP-2 for their ability to activate the cAMP generating system in rat gastric glands and HGT-1 human gastric cancer ...

journal_title：FEBS letters

authors： Hansen AB,Gespach CP,Rosselin GE,Holst JJ

更新日期：1988-08-15 00:00:00

abstract：:We characterized the promoter activity of a medaka fish intestinal guanylyl cyclase gene, OlGC6, by assay of enzyme activity in response to various promoter-luciferase fusion gene constructs introduced into CACO-2 cells and medaka fish embryos. A transient transfection assay of the various fusion gene constructs showe...

journal_title：FEBS letters

authors： Nakauchi M,Suzuki N

更新日期：2003-02-11 00:00:00

abstract：:Mammalian voltage-activated Shaker K+ channels associate with at least three cytoplasmic proteins: Kv beta 1, Kv beta 2 and Kv beta 3. These beta subunits contain variable N-termini, which can modulate the inactivation of Shaker alpha subunits, but are homologous throughout an aldo-keto reductase core. Human and ferre...

journal_title：FEBS letters

authors： McCormack K,McCormack T,Tanouye M,Rudy B,Stühmer W

更新日期：1995-08-14 00:00:00

abstract：:The interaction between two codon-anticodon duplexes of the ribosomal A- and P-site-bound tRNAs is the key feature of the proposed model. This interaction prohibits non-canonical base pairing at the first and second positions of the codon and controls base pairing at the third position (wobbling rules ensuing from the...

journal_title：FEBS letters

authors： Lim VI,Venclovas C

更新日期：1992-11-23 00:00:00

abstract：:Low-density lipoprotein receptor-related protein 4 (Lrp4) is essential for pre- and post-synaptic specialization at the neuromuscular junction (NMJ), an indispensable synapse between a motor nerve and skeletal muscle. Muscle-specific receptor tyrosine kinase MuSK must form a complex with Lrp4 to organize postsynaptic ...

journal_title：FEBS letters

authors： Hoshi T,Tezuka T,Yokoyama K,Iemura S,Natsume T,Yamanashi Y

更新日期：2013-11-29 00:00:00

abstract：:A key step in sulfate assimilation into cysteine is the reduction of sulfite to sulfide by sulfite reductase (SiR). This enzyme is encoded by three genes in the moss Physcomitrella patens. To obtain a first insight into the roles of the individual isoforms, we deleted the gene encoding the SiR1 isoform in P. patens by...

journal_title：FEBS letters

authors： Wiedemann G,Hermsen C,Melzer M,Büttner-Mainik A,Rennenberg H,Reski R,Kopriva S

更新日期：2010-06-03 00:00:00

abstract：:The (beta alpha)(8)-barrel is the most frequent and most versatile fold among enzymes [Höcker et al., Curr. Opin. Biotechnol. 12 (2001) 376-381; Wierenga, FEBS Lett. 492 (2001) 193-198]. Structural and functional evidence suggests that (beta alpha)(8)-barrels evolved from an ancestral half-barrel, which consisted of f...

journal_title：FEBS letters

authors： Höcker B,Schmidt S,Sterner R

更新日期：2002-01-16 00:00:00

abstract：:Our previous study documented the expression of Mta1 during spermatogenesis. Here, we present evidence for a possible involvement of Mta1 in the regulation of testicular function, possibly by interacting with p53. A notable decrease of Mta1 expression was revealed at postsurgical day 6, consistent with the previously ...

journal_title：FEBS letters

authors： Li W,Bao W,Ma J,Liu X,Xu R,Wang RA,Zhang Y

更新日期：2008-03-19 00:00:00

abstract：:Regulation of acetylcholine receptor (AChR) gene expression was analyzed in alpha-bungarotoxin (alpha-BTX) treated rats. A reduction in available 125I-alpha-BTX binding sites was accompanied by an increase in the various AChR transcripts. The increase in the AChR alpha-, beta- epsilon- and delta-subunit mRNAs was simi...

journal_title：FEBS letters

authors： Asher O,Provenzano C,Fuchs S

更新日期：1991-05-06 00:00:00

abstract：:The sialic acid of complex N-glycans can be biochemically engineered by substituting the physiological precursor N-acetylmannosamine with non-natural N-acylmannosamines. The Kv3.1 glycoprotein, a neuronal voltage-gated potassium channel, contains sialic acid. Western blots of the Kv3.1 glycoprotein isolated from trans...

journal_title：FEBS letters

authors： Hall MK,Reutter W,Lindhorst T,Schwalbe RA

更新日期：2011-10-20 00:00:00

abstract：:Evidence is presented here that cupric ions play a part in the natural spinning of Bombyx mori silk. Proton induced X-ray emission studies revealed that the copper content increased from the posterior part to the anterior part of silk gland, and then further increased in the silk fiber. Spectrophotometric analysis dem...

journal_title：FEBS letters

authors： Zhou L,Chen X,Shao Z,Zhou P,Knight DP,Vollrath F

更新日期：2003-11-20 00:00:00

abstract：:Mitogen-activated protein kinase (MAPK) cascades play an important role in mediating stress responses in plants. In Arabidopsis, 20 MAPKs have been identified and classified into four major groups (A-D). Little is known about the role of group C MAPKs. We have studied the activation of Arabidopsis subgroup C1 MAPKs (A...

journal_title：FEBS letters

authors： Ortiz-Masia D,Perez-Amador MA,Carbonell J,Marcote MJ

更新日期：2007-05-01 00:00:00