Interaction of a human blood group Sd(a-) Tamm-Horsfall glycoprotein with applied lectins.


:Unlike the human blood group Sd(a+) Tamm-Horsfall glycoprotein (THGP), the Sd(a-) one lacks terminal GalNAcbeta1--> residues at the nonreducing ends. The binding properties of this glycoprotein and its asialo product with lectins were characterized by quantitative precipitin (QPA) and precipitin inhibition assays. Among 20 lectins tested by QPA, both native and asialo Sd(a-) THGP reacted best with Abrus precatorius and Ricinus communis and completely precipitated the lectin added. They also precipitated well Wistaria floribunda (WFA), Glycine max (SBA), Bauhinia purpurea alba, abrin-a and ricin, all of which recognize the Galbeta1--> 4GlcNAcbeta1--> sequence, although at different strength. The lectin-glycan interactions were inhibited by Galbeta1--> 4GlcNAc and Galbeta1--> 4Glc. When the precipitability of Sd(a-) THGP was compared with that of the Sd(a+) phenotype, the native Sd(a-) THGP exhibited a 40% lesser affinity for WFA, SBA, WGA and mistletoe lectin-I (ML-I). Mapping the precipitation and inhibition profiles of the present study and the results of THGP Sd(a+), it is concluded that Sd(a-) THGP showed a strongly diminished affinity for GalNAcbeta1--> active lectins (SBA and WFA) than the Sd(a+) phenotype.




FEBS letters


Wu JH,Watkins WM,Chen CP,Song SC,Wu AM




Has Abstract


1996-04-22 00:00:00














  • 4-Hydroxyphenethyl alcohol--a new cytokinin-like substance from the phototrophic purple bacterium Rhodospirillum rubrum 1R.

    abstract::Three compounds with cytokinin activity have been isolated from the medium of Rhodospirillum rubrum grown photosynthetically. Two N-6 aminopurine cytokinins revealed in the medium were identical with those obtained from R. rubrum cells previously. The third compound with cytokinin activity in Amaranthus caudatus bioas...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Serdyuk OP,Smolygina LD,Muzafarov EN,Adanin VM,Arinbasarov MU

    更新日期:1995-05-22 00:00:00

  • Candida albicans PHO81 is required for the inhibition of hyphal development by farnesoic acid.

    abstract::Farnesoic acid is a signaling molecule that inhibits the transition from budding yeast to filament formation in Candida albicans, but the molecular mechanism regulated by this substance is unknown. In this study, we analyzed the function of CaPHO81, which is induced by farnesoic acid. The pho81Δ mutant cells existed e...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chung SC,Kim TI,Ahn CH,Shin J,Oh KB

    更新日期:2010-11-19 00:00:00

  • Repression of the c-fms gene in fibroblast cells by c-Myc-MM-1-TIF1beta complex.

    abstract::MM-1 has been reported to repress the E-box-dependent transcription activity of c-Myc by recruiting histone deacetylase 1 complex via TIF1beta/KAP1. In this study, to identify target genes for c-Myc-MM-1-TIF1beta, we established rat-1 cells harboring the dominant-negative form of TIF1beta to abrogate the pathway from ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Satou A,Hagio Y,Taira T,Iguchi-Ariga SM,Ariga H

    更新日期:2004-08-13 00:00:00

  • Substrate interactions in cytochrome P-450: correlation between carbon-13 nuclear magnetic resonance chemical shifts and C-O vibrational frequencies.

    abstract::13CO NMR chemical shifts and 12CO infrared stretching frequencies have been measured for cytochrome P-450cam-CO in the presence of D-camphor and of various camphor analogues. A linear correlation between both parameters delta (13C) and v(CO) was found indicating that the steric and electrostatic interactions acting on...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Legrand N,Bondon A,Simonneaux G,Jung C,Gill E

    更新日期:1995-05-08 00:00:00

  • The dual effects of the astrocyte-specific enhancer of the AQP4 M1 promoter.

    abstract::Aquaporin-4, a predominant water channel in the central nervous system, has two isoforms, M1 and M23, whose transcripts are driven by distinct promoters. Using a reporter assay, we found that a fragment located between exons 0 and 1 of the mouse aquaporin-4 gene, which had been thought to be the promoter for M23, lack...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Abe Y,Goda W,Ikeshima-Kataoka H,Yasui M

    更新日期:2017-12-01 00:00:00

  • Endurance training alters alanine and glutamine release from muscle during contractions.

    abstract::The release of alanine and glutamine from perfused muscle of trained and control animals was investigated. Release rates did not differ between trained and control muscle at rest. During contractions in trained muscle, alanine release was higher than at rest, while glutamine release was transiently increased. Phenylal...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hood DA,Terjung RL

    更新日期:1994-03-07 00:00:00

  • Skeletal muscle UCP2 and UCP3 gene expression in a rat cancer cachexia model.

    abstract::Rats bearing the Yoshida AH-130 ascites hepatoma showed an increased expression of both uncoupling protein-2 (UCP2) (194%) and UCP3 (189%) mRNA levels in skeletal muscle 7 days after tumour inoculation. Interestingly, an even greater increase was observed in mRNA for both UCP2 (278%) and UCP3 (797%) in the pair-fed an...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sanchís D,Busquets S,Alvarez B,Ricquier D,López-Soriano FJ,Argilés JM

    更新日期:1998-10-09 00:00:00

  • 4-Picoline-2,2':6',2"-terpyridine-platinum(II) - a potent intercalator of DNA.

    abstract::4-Picoloine-2,2':6',2"-terpyridine-platinum(II) is shown in a ligation assay to unwind and so intercalate into DNA. Circular dichroism is used to determine an equilibrium binding constant of approximately 2 x 10(7) M(-1) for the most stable binding mode of 4-picoline-2,2':6',2"-terpyridine-platinum(II) to poly[d(A-T)2...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: McCoubrey A,Latham HC,Cook PR,Rodger A,Lowe G

    更新日期:1996-02-12 00:00:00

  • Pyroglutamate stimulates Na+ -dependent glutamate transport across the blood-brain barrier.

    abstract::Regulation of Na(+)-dependent glutamate transport was studied in isolated luminal and abluminal plasma membranes derived from the bovine blood-brain barrier. Abluminal membranes have Na(+)-dependent glutamate transporters while luminal membranes have facilitative transporters. This organization allows glutamate to be ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hawkins RA,Simpson IA,Mokashi A,Viña JR

    更新日期:2006-08-07 00:00:00

  • Acylation of adenylyl cyclase catalyst is important for enzymic activity.

    abstract::Incubation of human thrombocytes in the presence of [3H]palmitic acid leads to incorporation of this fatty acid into the alpha subunit of Gs as described [Linder et al., Proc. Natl. Acad. Sci. USA 90 (1993) 3675-3679; Degtyarev et al., Biochemistry 32 (1993) 8057-8061] but also into the catalyst of adenylyl cyclase wh...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mollner S,Beck K,Pfeuffer T

    更新日期:1995-09-11 00:00:00

  • Multisite phosphorylation of the glycogen-binding subunit of protein phosphatase-1G by cyclic AMP-dependent protein kinase and glycogen synthase kinase-3.

    abstract::The glycogen-binding (G) subunit of protein phosphatase-1G is phosphorylated stoichiometrically by glycogen synthase kinase-3 (GSK3), and with a greater catalytic efficiency than glycogen synthase, but only after prior phosphorylation by cyclic AMP-dependent protein kinase (A-kinase) at site 1. The residues phosphoryl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dent P,Campbell DG,Hubbard MJ,Cohen P

    更新日期:1989-05-08 00:00:00

  • New potent inhibitors of aquaporins: silver and gold compounds inhibit aquaporins of plant and human origin.

    abstract::Silver and gold compounds were tested as potential inhibitors of aquaporins of plant- and human origin. Silver as AgNO(3) or silver sulfadiazine inhibited with high potency (EC(50) 1-10 microM) the water permeability of the peribacteroid membrane from soybean (containing Nodulin 26), the water permeability of plasma m...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Niemietz CM,Tyerman SD

    更新日期:2002-11-20 00:00:00

  • Regulation of the F0F1-ATP synthase: the conformation of subunit epsilon might be determined by directionality of subunit gamma rotation.

    abstract::F(0)F(1)-ATP synthase couples ATP synthesis/hydrolysis with transmembrane proton transport. The catalytic mechanism involves rotation of the gamma epsilon c(approximately 10)-subunits complex relative to the rest of the enzyme. In the absence of protonmotive force the enzyme is inactivated by the tight binding of MgAD...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Feniouk BA,Junge W

    更新日期:2005-09-26 00:00:00

  • Trendspotting in the Protein Data Bank.

    abstract::The Protein Data Bank (PDB) was established in 1971 as a repository for the three dimensional structures of biological macromolecules. Since then, more than 85000 biological macromolecule structures have been determined and made available in the PDB archive. Through analysis of the corpus of data, it is possible to id...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Berman HM,Coimbatore Narayanan B,Di Costanzo L,Dutta S,Ghosh S,Hudson BP,Lawson CL,Peisach E,Prlić A,Rose PW,Shao C,Yang H,Young J,Zardecki C

    更新日期:2013-04-17 00:00:00

  • Soluble, nitrate/nitrite-inducible cytochrome P-450 of the fungus, Fusarium oxysporum.

    abstract::Both soluble and microsomal fractions of Fusarium oxysporum contain cytochrome P-450(P-450). We report here that the P-450 in the soluble fraction was induced only when nitrate or nitrite was added to the growth medium, whereas the microsomal P-450 was synthesized regardless of the medium compositions. The reduced-CO ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shoun H,Suyama W,Yasui T

    更新日期:1989-02-13 00:00:00

  • The 'assembly-promoting sequence region' of microtubule-associated protein 4 failed to promote microtubule assembly.

    abstract::In order to study the function of the bovine MAP4 microtubule-binding domain (the assembly-promoting (AP) sequence region), a fragment corresponding to the AP sequence region was prepared using an Escherichia coli expression system. When the fragment was mixed with purified tubulin at 37 degrees C, the fragment caused...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Katsuki M,Tokuraku K,Nakagawa H,Murofushi H,Kotani S

    更新日期:1997-11-24 00:00:00

  • Flanking aromatic residue competition influences transmembrane peptide helix dynamics.

    abstract::To address biophysical principles and lipid interactions that underlie the properties of membrane proteins, modifications that vary the neighbors of tryptophan residues in the highly dynamic transmembrane helix of GW4,20 ALP23 (acetyl-GGAW4 A(LA)6 LAW20 AGA-amide) were examined using deuterium NMR spectroscopy. It was...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: McKay MJ,Greathouse DV,Koeppe RE 2nd

    更新日期:2020-12-01 00:00:00

  • Differential activation of STATs 3 and 5 during mammary gland development.

    abstract::We have investigated the activity of STAT family members throughout a mammary developmental cycle. Transcripts for Stat 5 were upregulated during pregnancy whilst STAT1 and STAT3 mRNAs were expressed at constant levels. DNA binding complexes containing both STAT5a and 5b showed differing affinities for two naturally o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Philp JA,Burdon TG,Watson CJ

    更新日期:1996-10-28 00:00:00

  • Search for a dextransucrase minimal motif involved in dextran binding.

    abstract::Fourteen truncated forms of Leuconostoc mesenteroides NRRL B512-F dextransucrase, involving N-, C- or N- plus C-terminal domain truncations were tested for their ability to bind dextrans. The shortest fragment (14kDa molecular weight) that still exhibited a strong interaction with dextran was localized between amino a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Suwannarangsee S,Moulis C,Potocki-Veronese G,Monsan P,Remaud-Simeon M,Chulalaksananukul W

    更新日期:2007-10-02 00:00:00

  • Effects of pressure on glucose transport in human erythrocytes.

    abstract::The operation of the human red cell glucose transporter has been studied at normal and high hydrostatic pressure to identify the step(s) which involve a volume change. Pressure inhibited zero-trans and equilibrium exchange influx to similar extents, by decreasing the Vmax but not significantly changing the Km. The Bma...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Thorne SD,Hall AC,Lowe AG

    更新日期:1992-04-27 00:00:00

  • Non-radioactive reverse transcriptase/polymerase chain reaction for quantification of myosin heavy chain mRNA isoforms in various rabbit muscles.

    abstract::A method was established for measuring molecule numbers of three different myosin heavy chain (MHC) mRNA isoforms in total RNA preparations. The quantification was based on a combination of primer-directed reverse transcriptase and polymerase chain reactions with 5'-digoxigenin-labeled oligonucleotides, using external...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Peuker H,Pette D

    更新日期:1993-03-08 00:00:00

  • Low-conductance chloride channel activated by cAMP in the epithelial cell line T84.

    abstract::We have studied the modulation and pharmacological properties of two anion channels in T84 cells by recording single channel and transepithelial currents. One channel had an outwardly rectifying current-voltage I/V curve, was rarely active in cell-attached patches, and was unaffected by cAMP. The other channel had low...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tabcharani JA,Low W,Elie D,Hanrahan JW

    更新日期:1990-09-17 00:00:00

  • Movement of the 3'-end of 16 S RNA towards S21 during activation of 30 S ribosomal subunits.

    abstract::Fluorescence techniques were used to study conformational changes that occur in inactive E. coli 30 S ribosomal subunits during activation by heating in 12 mM Mg2+. Activation is associated with movement of a fluorophore on the 3'-end of 16 S RNA into a less polar environment and towards a probe on the cysteine thiol ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Odom OW,Stöffler G,Hardesty B

    更新日期:1984-07-23 00:00:00

  • Ion channels in autoimmune neurodegeneration.

    abstract::Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system characterized by widespread inflammation, focal demyelination and a variable degree of axonal and neuronal loss. Ionic conductances regulate T cell activation as well as neuronal function and thus have been found to play a crucial ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Ehling P,Bittner S,Budde T,Wiendl H,Meuth SG

    更新日期:2011-12-01 00:00:00

  • Hypoxia induces apoptosis in human neuroblastoma SK-N-MC cells by caspase activation accompanying cytochrome c release from mitochondria.

    abstract::We have attempted to elucidate the mechanism of apoptotic cell death induced by hypoxia (very low oxygen conditions) in neuronal cells. Human neuroblastoma SK-N-MC cells under hypoxic conditions resulted in apoptosis in a time-dependent manner estimated by DNA fragmentation assay and nuclear morphology stained with fl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Araya R,Uehara T,Nomura Y

    更新日期:1998-11-13 00:00:00

  • Involvement of decreased myo-inositol transport in lipopolysaccharide-induced depression of phosphoinositide hydrolysis in vascular smooth muscle.

    abstract::The mechanism underlying lipopolysaccharide (LPS)-induced depression of phosphoinositide (PI) hydrolysis was investigated using rat aortas. In LPS-pretreated aortas, the 5-hydroxytryptamine-stimulated accumulation of inositol monophosphate and incorporation of exogenous myo-inositol into PIs were significantly less th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sotoda Y,Negoro M,Wakabayashi I

    更新日期:2002-05-22 00:00:00

  • Transforming growth factor-beta1 induces activation of Ras, Raf-1, MEK and MAPK in rat hepatic stellate cells.

    abstract::The transdifferentiation of hepatic stellate cells into myofibroblast-like cells and the proliferation of the transdifferentiated cells are controlled by TGF-beta1. Little is known about the intracellular signal transducers of TGF-beta1. In this paper we show that in cultured hepatic stellate cells TGF-beta1 induces a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Reimann T,Hempel U,Krautwald S,Axmann A,Scheibe R,Seidel D,Wenzel KW

    更新日期:1997-02-10 00:00:00

  • SRC transcriptional activation in a subset of human colon cancer cell lines.

    abstract::Human SRC encodes the non-receptor tyrosine kinase pp60(c-Src), which is activated in many human colon cancer cell lines (HCCLs) and tumors. We found that both c-Src protein and mRNA levels were elevated in a subset of HCCLs. Increased c-Src mRNA and protein levels correlated strongly with increased c-Src kinase activ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dehm S,Senger MA,Bonham K

    更新日期:2001-01-05 00:00:00

  • Caenorhabditis elegans DLC-1 associates with ribonucleoprotein complexes to promote mRNA regulation.

    abstract::Ribonucleoprotein complexes, which contain mRNAs and their regulator proteins, carry out post-transcriptional control of gene expression. The function of many RNA-binding proteins depends on their association with cofactors. Here, we use a genomic approach to identify transcripts associated with DLC-1, a protein previ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Day NJ,Ellenbecker M,Voronina E

    更新日期:2018-11-01 00:00:00

  • A large conductance ion channel in the nuclear envelope of a higher plant cell.

    abstract::To detect and characterize ion channel activity in the nuclear envelope of a higher plant cell, we performed patch clamp experiments on nuclei isolated from coconut endosperm cells and on giant liposomes containing nuclear envelope fragments prepared from the same cells. An ion channel exhibiting a number of conductan...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Matzke AJ,Behensky C,Weiger T,Matzke MA

    更新日期:1992-05-04 00:00:00