Abstract:
:Smad ubiquitination regulatory factor 2 (Smurf2), a ubiquitin ligase for Smads, plays critical roles in the regulation of transforming growth factor-beta (TGF-beta)-Smad signaling via ubiquitin-dependent degradation of Smad2 and Smad7. We found that TGF-beta stimulates Smurf2 expression. TGF-beta activated the Smurf2 promoter in a TGF-beta responsive cell lines, whereas IL-1alpha, PDGF and epidermal growth factor did not. TGF-beta-mediated Smurf2 promoter activation was inhibited by Smad7 or an activin receptor-like kinase 5 inhibitor but not by dominant negative Smad or disruption of Smad-binding elements in the promoter. Moreover, inhibition of the phosphatidil inositol 3 kinase (PI3K)/Akt pathway suppressed TGF-beta-mediated Smurf2 induction. These results suggest that TGF-beta stimulates Smurf2 expression by Smad-independent pathway such as PI3K/Akt pathway via TGF-beta receptor.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ohashi N,Yamamoto T,Uchida C,Togawa A,Fukasawa H,Fujigaki Y,Suzuki S,Kitagawa K,Hattori T,Oda T,Hayashi H,Hishida A,Kitagawa Mdoi
10.1016/j.febslet.2005.03.069keywords:
subject
Has Abstractpub_date
2005-05-09 00:00:00pages
2557-63issue
12eissn
0014-5793issn
1873-3468pii
S0014-5793(05)00421-7journal_volume
579pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::We amplified, using the polymerase chain reaction and calcitonin receptor (CTR) specific primers, RNA extracted from medullary thyroid carcinoma (MTC) and the derived TT cell line. Both secrete large amounts of calcitonin. Electrophoresis of amplification products revealed, in both cases, an ethidium bromide-stained b...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80503-2
更新日期:1994-04-04 00:00:00
abstract::Glycine amide (GlyAd), a typically amidated amino acid, is a versatile additive that suppresses protein aggregation during refolding, heat treatment, and crystallization. In spite of its effectiveness, the exact mechanism by which GlyAd suppresses protein aggregation remains to be elucidated. Here, we show the crystal...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.01.008
更新日期:2011-02-04 00:00:00
abstract::The structures of the M110 and M21 regulatory subunits of protein phosphatase-1M, the major enzyme which dephosphorylates myosin in smooth muscle, have been deduced from cloned cDNAs. The N-terminus of the M110 subunit from rat aorta contains seven ankyrin repeats, while the C-terminus of the M21 subunit from chicken ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01231-8
更新日期:1994-12-12 00:00:00
abstract::Methylation of cytosine-residues in the sequence CpG affects the expression of many genes and generally correlates with reduced transcription. The ribosomal genes of Xenopus laevis were among the first genes to be studied with respect to their DNA methylation, and a loss of methylation during embryonic development cor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01291-1
更新日期:1994-12-19 00:00:00
abstract::Plasminogen activator inhibitor-1 (PAI-1) increases injury in several liver, lung and kidney disease models. The objective of this investigation was to assess the effect of PAI-1 deficiency on cholestatic liver fibrosis and determine PAI-1 influenced fibrogenic mechanisms. We found that PAI-1(-/-) mice had less fibros...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.05.049
更新日期:2007-06-26 00:00:00
abstract::The low density lipoprotein receptor-related protein (LRP) is a multifunctional cell surface receptor highly expressed in human aortic smooth muscle cells. In the present study, we used the short interfering RNA (siRNA) technique to explore the role of LRP in smooth muscle cell migration. We identified an LRP-specific...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01272-9
更新日期:2003-12-04 00:00:00
abstract::Gustatory papillae and associated taste buds receive and process chemical information from the environment. In mammals, their development takes place during the late phase of embryogenesis. However, the cellular factors that regulate the differentiation of taste papillae remain largely unknown. Here, we show by quanti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.08.078
更新日期:2006-10-02 00:00:00
abstract::Vesicular monoamine transporters (VMAT) catalyze transport of serotonin, dopamine, epinephrine and norepinephrine into subcellular storage organelles in a variety of cells. Accumulation of the neurotransmitter depends on the proton electrochemical gradient across the organelle membrane and involves VMAT-mediated excha...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01252-0
更新日期:1994-12-12 00:00:00
abstract::African horse sickness virus (AHSV), a member of the orbivirus genus of the family Reoviridae, is an insect-vectored pathogen of horses of concern to the equine industry. Studies on AHSV replication and pathogenesis have been hampered by the lack of reverse genetics allowing targeted mutation of viral genomes. We demo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.06.030
更新日期:2010-08-04 00:00:00
abstract::A high-throughput cell-free protein synthesis method has been described. The methodology is based on a bilayer diffusion system that enables the continuous supply of substrates, together with the continuous removal of small byproducts, through a phase between the translation mixture and substrate mixture. With the use...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02329-3
更新日期:2002-03-06 00:00:00
abstract::Prominin-1 (CD133) is a cholesterol-interacting pentaspan membrane protein concentrated in plasma membrane protrusions. In epithelial cells, notably neuroepithelial stem cells, prominin-1 is found in microvilli, the primary cilium and the midbody. These three types of apical membrane protrusions are subject to remodel...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.01.050
更新日期:2010-05-03 00:00:00
abstract::Single channel recording techniques have been applied to neurons cultured from the hippocampus and the respiratory area of the brain stem of fetal rats in order to search for nicotinic acetylcholine receptors (nAChR) in the central nervous system. In addition to acetylcholine (ACh), the potent and specific agonist (+)...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80192-8
更新日期:1987-09-28 00:00:00
abstract::Piriprost and nordihydroguiaretic acid (NDGA), specific inhibitors of arachidonate lipoxygenase, inhibited phytohaemagglutinin (PHA)-stimulated breakdown of inositol lipids in human T lymphocytes. The dual inhibitors eicosatetraynoic acid (ETYA) and BW 755C, which inhibit both lipoxygenase and cyclooxygenase, also had...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81621-7
更新日期:1989-11-20 00:00:00
abstract::The flash-induced voltage response of halorhodopsin at high NaCl concentration comprises two main kinetic components. The first component with tau approximately 1 micros does not exceed 4% of the overall response amplitude and is probably associated with the formation of the L (hR520) intermediate. The second main com...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00394-9
更新日期:1998-05-01 00:00:00
abstract::Originally identified as multicopy suppressor of a lethal growth arrest caused by expression of a tumour mutant cDNA of p53 in fission yeast the tms1 gene product was found to form stable complexes with p53 in yeast. By using purified recombinant proteins multimeric complexes of tms1 and p53 could be demonstrated and ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01329-6
更新日期:1995-12-18 00:00:00
abstract::Canine aortic strip studies revealed insensitivity of angiotensin II (AII)-induced aortic contraction to inhibition by the non-peptide antagonist DuP753 (pKB = 6.7 +/- 0.1). In order to determine the origin of this phenomenon we cloned the canine homologue of the AT1 AII receptor. Expression of this cDNA in COS-7 cell...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80307-2
更新日期:1994-04-25 00:00:00
abstract::We report that sodium butyrate, a natural product of fiber degradation by colonic bacteria, markedly suppresses c-Myc-mediated apoptosis induced in murine plasmacytomas and human Burkitt lymphomas by growth factor deprivation, but not in cell lines devoid of c-myc translocations. Attenuation of cell death is associate...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00926-0
更新日期:1998-08-28 00:00:00
abstract::The dynamics of chromatin structure are tightly regulated by multiple epigenetic mechanisms such as histone modifications and incorporation of histone variants. In the current work, differentiation of an embryonal carcinoma cell line, NT2, was induced by retinoic acid, and total histone proteins were compared througho...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.10.041
更新日期:2010-11-19 00:00:00
abstract::Temporary inhibition of the cysteine proteinases papain and cathepsin L was observed with several hairpin loop mutants of recombinant chicken cystatin at enzyme concentrations above nanomolar. Kinetic modelling of inhibition data, gel electrophoresis and amino acid sequencing revealed that reappearance of papain activ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00174-8
更新日期:1995-03-20 00:00:00
abstract::Mitochondria continually fuse and divide to dynamically adapt to changes in metabolism and stress. Mitochondrial dynamics are also required for mitochondrial DNA (mtDNA) integrity; however, the underlying reason is not known. In this study, we examined the link between mitochondrial fusion and mtDNA maintenance in Sch...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12432
更新日期:2016-10-01 00:00:00
abstract::A novel peptide from Centruroides noxius Hoffmann scorpion venom was isolated and sequenced. The 37 amino acid peptide belongs to the charybdotoxin sub-family (alphaKTx1) and was numbered member 11. alphaKTx1.11 has 75% sequence identity with iberiotoxin and 54% with charybdotoxin. alphaKTx1.11 revealed specificity fo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02791-0
更新日期:2001-09-21 00:00:00
abstract::The kinetics of AMP-aminohydrolase, which under steady state conditions shows a typical sigmoid dependence of initial velocities versus substrate concentration, have been examined by rapid mixing methods. Using this technique it was observed that when substrate or substrate plus activator (K(+)) were mixed with enzyme...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(68)80102-4
更新日期:1968-11-01 00:00:00
abstract::The polytopic transmembrane protein, Niemann-Pick C1-Like 1 (NPC1L1), is enriched in the apical membrane of small intestine absorptive enterocytes where it mediates extracellular sterol transport across the brush border membrane. It is essential for intestinal sterol absorption and is the molecular target of ezetimibe...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.03.030
更新日期:2010-07-02 00:00:00
abstract::Electron paramagnetic resonance (EPR) and near-infrared magnetic circular dichroism (MCD) have been used to identify the ligands to the cytochrome b556 component of succinate: ubiquinone oxidoreductase (succinate dehydrogenase) from Escherichia coli. The 'highly axial low spin' (HALS) EPR spectrum suggests bis(histidi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01189-3
更新日期:1994-11-28 00:00:00
abstract::Piericidin is a potent inhibitor of the mitochondrial and bacterial type I NADH-ubiquinone oxidoreductases (Complex I) and is considered to bind at or close to the ubiquinone binding site(s) of the enzyme. Piericidin-resistant mutants of the bacterium Rhodobacter capsulatus have been isolated and the present work demo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00719-4
更新日期:1998-07-10 00:00:00
abstract::Pexophagy is a selective autophagy process wherein damaged and/or superfluous peroxisomes undergo vacuolar degradation. In methylotropic yeasts, where pexophagy has been studied most extensively, this process occurs by either micro- or macropexophagy: processes analogous to micro- and macroautophagy. Recent studies ha...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.01.019
更新日期:2010-04-02 00:00:00
abstract::Treatment of Chinese hamster ovary K1 cells with phosphatidylserine (PS) caused typical apoptosis with distinct morphological and biochemical features in a dose- and time-dependent manner. However, unlike camptothecin-induced apoptosis, changes in mitochondrial transmembrane potential were not observed. In addition, c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02771-5
更新日期:2001-08-24 00:00:00
abstract::In the brain inwardly rectifying potassium channel Kir7.1 subunits are predominantly expressed in the choroid plexus and meninges. To investigate this tissue-specific expression pattern, we characterized the genomic organization and the 5' proximal promoter of the rat Kir7.1 gene (Kcnj13). Starting from the major tran...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02092-5
更新日期:2000-10-20 00:00:00
abstract::The Ca2+-binding parvalbumin has been purified for the first time from rat kidney. Its biochemical and immunological properties were indistinguishable from the muscle counterpart. By immunohistochemical methods parvalbumin was localized in part of the distal tubule and proximal collecting duct, similar to the vitamin ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80569-5
更新日期:1986-05-26 00:00:00
abstract::We transfected the COS-7 cells with cDNAs encoding different human somatostatin receptor (hSSTR) subtypes, and found that hSSTR subtypes mediate not only the inhibition of forskolin-induced cAMP accumulation but also the stimulation of phospholipase C (PLC) and Ca2+ mobilization. Activation of PLC by 1 microM somatost...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00603-2
更新日期:1994-07-11 00:00:00