Abstract:
:Treatment of Chinese hamster ovary K1 cells with phosphatidylserine (PS) caused typical apoptosis with distinct morphological and biochemical features in a dose- and time-dependent manner. However, unlike camptothecin-induced apoptosis, changes in mitochondrial transmembrane potential were not observed. In addition, cytochrome c release did not occur in PS-induced apoptosis. A pan caspase inhibitor, Z-VAD, significantly inhibited the apoptosis, but inhibitors of caspase-1, -3, -8 and -9 did not. Activities of caspase-1, -3, -8 and -9 were increased by treatment of the cells with camptothecin, but not with PS. These results suggest that PS-induced apoptosis occurs without the collapse of mitochondrial transmembrane potential and without the release of cytochrome c, in a manner independent of caspase-1, -3, -8 and -9.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Miyato Y,Ibuki Y,Ohyama H,Yamada T,Goto Rdoi
10.1016/s0014-5793(01)02771-5keywords:
subject
Has Abstractpub_date
2001-08-24 00:00:00pages
73-7issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(01)02771-5journal_volume
504pub_type
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