Transduction of Cu,Zn-superoxide dismutase mediated by an HIV-1 Tat protein basic domain into mammalian cells.

abstract：:The human gut microbiota comprises approximately 100 trillion microbial cells and has a significant effect on many aspects of human physiology including metabolism, nutrient absorption and immune function. Disruption of this population has been implicated in many conditions and diseases, including examples such as obe...

journal_title：FEBS letters

authors： Walsh CJ,Guinane CM,O'Toole PW,Cotter PD

更新日期：2014-11-17 00:00:00

abstract：:One key area of protein kinase research is the identification of cognate substrates. The search for substrates is hampered by problems in unambiguously assigning substrates to a particular kinase in vitro and in vivo. One solution to this impasse is to engineer the kinase of interest to accept an ATP analogue which is...

journal_title：FEBS letters

authors： Hindley AD,Park S,Wang L,Shah K,Wang Y,Hu X,Shokat KM,Kolch W,Sedivy JM,Yeung KC

更新日期：2004-01-02 00:00:00

abstract：:Reaction of ubiquinone in the high-affinity quinone-binding site (QH) in bo-type ubiquinol oxidase from Escherichia coli was revealed by EPR and optical studies. In the QH site, ubiquinol was shown to be oxidized to ubisemiquinone and to ubiquinone, while no semiquinone signal was detected in the oxidase isolated from...

journal_title：FEBS letters

authors： Sato-Watanabe M,Itoh S,Mogi T,Matsuura K,Miyoshi H,Anraku Y

更新日期：1995-10-30 00:00:00

abstract：:SV40 large tumor-antigen (T-ag) nuclear import is enhanced by the protein kinase CK2 (CK2) site (Ser111Ser112) flanking the nuclear localization sequence (NLS). Here we use site-directed mutagenesis to examine the influence of negative charge and conformation at the site on T-ag nuclear import and recognition by the N...

journal_title：FEBS letters

authors： Xiao CY,Jans P,Jans DA

更新日期：1998-12-04 00:00:00

abstract：:The yeast integral plasma membrane protein Ist2 belongs to a group of membrane proteins which are synthesized from localized mRNAs. The protein reaches the plasma membrane via the ER on a route operating independently of the classical secretory pathway. We have identified a complex peptide-sorting signal located at th...

journal_title：FEBS letters

authors： Franz A,Maass K,Seedorf M

更新日期：2007-02-06 00:00:00

abstract：:The human H19 gene is a paternally imprinted oncofetal gene, highly expressed in several fetal tissues, down-regulated in nearly all adult tissues but re-expressed in carcinomas of tissues which express the gene in fetal life. It has no known protein product and till today, no function could be designated to H19 RNA. ...

journal_title：FEBS letters

authors： Ohana P,Kopf E,Bibi O,Ayesh S,Schneider T,Laster M,Tykocinski M,de Groot N,Hochberg A

更新日期：1999-07-02 00:00:00

abstract：:The 36 kDa fragment of actin molecule obtained with the protease from E. coli A2 strain [(1988) FEBS Lett. 228, 172] was shown to begin with Val-43 and retain the COOH-terminal amino acid residues of the parent molecule. The E. coli protease split actin preserves the NH2-terminal part of the polypeptide chain as well ...

journal_title：FEBS letters

authors： Khaitlina SYu,Collins JH,Kuznetsova IM,Pershina VP,Synakevich IG,Turoverov KK,Usmanova AM

更新日期：1991-02-11 00:00:00

abstract：:Recent studies have implicated that a GTP-binding protein (G-protein) is involved in the coupling of both CCK-8 and muscarinic cholinergic receptors to phosphoinositidase C (PIC) in the human embryonic pituitary cell line, Flow 9000. Pretreatment of these cells with cholera toxin, but not pertussis toxin, inhibited th...

journal_title：FEBS letters

authors： Lo WW,Hughes J

更新日期：1987-08-17 00:00:00

abstract：:In dog-fish spermatid nuclei two intermediate proteins S1 and S2 replace histones before the setting down of protamines. These spermatid-specific proteins were isolated by carboxymethyl-cellulose chromatography and purified by high pressure liquid chromatography. S1 and S2 are characterized by a high content of basic ...

journal_title：FEBS letters

authors： Chauviere M,Laine B,Sautiere P,Chevaillier P

更新日期：1983-02-21 00:00:00

abstract：:The L1CAM antibody A10-A3 efficiently reduces tumor growth in a nude mouse model. Here, we describe the crystal structure of the Fab fragment of A10-A3 determined at 2.0 angstrom resolution. The A10-A3 antibody H3 loop reveals a characteristic arrangement of exposed aromatic residues that may play an important role in...

journal_title：FEBS letters

authors： Wei CH,Lee ES,Jeon JY,Heo YS,Kim SJ,Jeon YH,Kim KH,Hong HJ,Ryu SE

更新日期：2011-01-03 00:00:00

abstract：:The Arabidopsis thalina genome database was searched for homologues of the Candida cloacae fao1 gene which encodes a membrane bound, flavin-containing, hydrogen peroxide generating, long chain alcohol oxidase. This gene has not been isolated from plants or animals. Four putative candidates were found in the database b...

journal_title：FEBS letters

authors： Cheng Q,Liu HT,Bombelli P,Smith A,Slabas AR

更新日期：2004-09-10 00:00:00

abstract：:HERC proteins are characterized by having one or more RCC1-like domains as well as a C-terminal HECT domain in their amino acid sequences. This has led researchers to suggest that they may act as both guanine nucleotide exchange factors and E3 ubiquitin ligases. Here we describe a physical interaction between the HECT...

journal_title：FEBS letters

authors： Garcia-Gonzalo FR,Cruz C,Muñoz P,Mazurek S,Eigenbrodt E,Ventura F,Bartrons R,Rosa JL

更新日期：2003-03-27 00:00:00

abstract：:The relationship between ground state cis-trans isomerization and protonation state is explored for a model green fluorescent protein chromophore, 4-hydroxybenzylidene-1,2-dimethylimidazolinone (HBDI). We find that the protonation state has only a modest effect on the free energy differences between cis and trans isom...

journal_title：FEBS letters

authors： He X,Bell AF,Tonge PJ

更新日期：2003-08-14 00:00:00

abstract：:Glycinebetaine (betaine), an osmoregulant in halophilic plants, stabilized the evolution of oxygen and the synthesis of ATP by thylakoid membranes from the cyanobacterium Synechocystis PCC6803 when it was present during the preparation and incubation of the thylakoid membranes. Moreover, betaine enhanced the evolution...

journal_title：FEBS letters

authors： Mamedov MD,Hayashi H,Wada H,Mohanty PS,Papageorgiou GC,Murata N

更新日期：1991-12-09 00:00:00

abstract：:A chloroperoxidase gene from Pseudomonas pyrrocinia was cloned into Escherichia coli using the cosmid vector pJB8. The gene coding for the chloroperoxidase could be localized to a 1.5 kb fragment of DNA which was subcloned into the high-copy-number plasmid pUC18. In one subclone increased halogenating activity could b...

journal_title：FEBS letters

authors： Wolfframm C,van Pée KH,Lingens F

更新日期：1988-10-10 00:00:00

abstract：:A key step in sulfate assimilation into cysteine is the reduction of sulfite to sulfide by sulfite reductase (SiR). This enzyme is encoded by three genes in the moss Physcomitrella patens. To obtain a first insight into the roles of the individual isoforms, we deleted the gene encoding the SiR1 isoform in P. patens by...

journal_title：FEBS letters

authors： Wiedemann G,Hermsen C,Melzer M,Büttner-Mainik A,Rennenberg H,Reski R,Kopriva S

更新日期：2010-06-03 00:00:00

abstract：:The insulin receptor family consists of the homologous tyrosine kinase receptors, insulin receptor (IR), insulin-like growth factor 1 receptor (IGF1R) and insulin receptor-related receptor. The three-dimensional structures of the tyrosine kinase domain of the IR and the first three extracellular domains (L1, Cys-rich ...

journal_title：FEBS letters

authors： Marino-Buslje C,Mizuguchi K,Siddle K,Blundell TL

更新日期：1998-12-18 00:00:00

abstract：:A new secondary structure model for parts of the 7 SL RNA is proposed which indicates for a stretch of at least 40 bases a strong structural homology to the ribosomal protein L5 binding site of eukaryotic 5 S rRNA. It is suggested that the 5 S rRNA-like structural part of 7 SL RNA mediates binding of the signal recogn...

journal_title：FEBS letters

authors： Boehm S

更新日期：1987-02-09 00:00:00

abstract：:Whether all inflammatory agents activate nuclear transcription factors NF-kappaB and activated protein-1 (AP-1) through the same mechanism is not known. We examined the effect of the phosphatidylinositol-3-kinase (PI-3K) inhibitor wortmannin on the activation of NF-kappaB and AP-1 by different inflammatory agents. Wor...

journal_title：FEBS letters

authors： Manna SK,Aggarwal BB

更新日期：2000-05-04 00:00:00

abstract：:The integrin alpha IIb beta 3-mediated redistribution of the tyrosine kinases pp125FAK and pp60Src and the small GTP-binding proteins CDC42Hs and Rap1B from the membrane skeleton to the cytoskeleton was found to be reversible: upon prolonged platelet aggregation (up to 15 min) induced by the thrombin-receptor activati...

journal_title：FEBS letters

authors： Dash D,Aepfelbacher M,Siess W

更新日期：1995-04-24 00:00:00

abstract：:Single rat hepatocytes microinjected with aequorin show free Ca oscillations when stimulated with Ca(2+)-mobilizing hormones. We show here that an inhibitor of diacylglycerol kinase (R 59 022) and an analogue of native diacylglycerol (diC8) inhibit free Ca oscillations induced by phenylephrine and vasopressin. These r...

journal_title：FEBS letters

authors： Sanchez-Bueno A,Cobbold PH

更新日期：1992-11-02 00:00:00

abstract：:Bisdioxopiperazine anti-cancer agents are catalytic inhibitors of topoisomerase II which by unknown means lock the enzyme in a closed clamp form and inhibit its ATPase activity. In order to demarcate a putative pharmacophore, we here describe a novel Tyr165Ser mutation in the enzyme's Walker A ATP binding site leading...

journal_title：FEBS letters

authors： Wessel I,Jensen LH,Renodon-Corniere A,Sorensen TK,Nitiss JL,Jensen PB,Sehested M

更新日期：2002-06-05 00:00:00

abstract：:Fas ligand (FasL) binds Fas (CD95) to induce apoptosis or activate other signaling pathways. In addition, FasL transduces bidirectional or 'reverse signals'. The intracellular domain of FasL contains consensus sequences for phosphorylation and an extended proline rich region, which regulate its surface expression thro...

journal_title：FEBS letters

authors： Thornhill PB,Cohn JB,Drury G,Stanford WL,Bernstein A,Desbarats J

更新日期：2007-09-18 00:00:00

abstract：:Immunoprecipitated p100Gag-Mil protein kinase from MH2-transformed quail embryo fibroblasts is associated with an 89 kDa protein. The molar ratio between p89 and Gag-Mil in the immunocomplex is 0.72, indicating that the majority of Gag-Mil is complexed with p89. During mitosis part of Gag-Mil is shifted to a form with...

journal_title：FEBS letters

authors： Lovrić J,Bischof O,Moelling K

更新日期：1994-04-18 00:00:00

abstract：:Smad ubiquitination regulatory factor 2 (Smurf2), a ubiquitin ligase for Smads, plays critical roles in the regulation of transforming growth factor-beta (TGF-beta)-Smad signaling via ubiquitin-dependent degradation of Smad2 and Smad7. We found that TGF-beta stimulates Smurf2 expression. TGF-beta activated the Smurf2 ...

journal_title：FEBS letters

authors： Ohashi N,Yamamoto T,Uchida C,Togawa A,Fukasawa H,Fujigaki Y,Suzuki S,Kitagawa K,Hattori T,Oda T,Hayashi H,Hishida A,Kitagawa M

更新日期：2005-05-09 00:00:00

abstract：:The white-rot fungus Phanerochaete chrysosporium has two intracellular beta-glucosidases (BGL1A and BGL1B) belonging to glycoside hydrolase (GH) family 1. BGL1B effectively hydrolyzes cellobiose and cellobionolactone, but BGL1A does not. We have determined the crystal structure of BGL1A in substrate-free and gluconola...

journal_title：FEBS letters

authors： Nijikken Y,Tsukada T,Igarashi K,Samejima M,Wakagi T,Shoun H,Fushinobu S

更新日期：2007-04-03 00:00:00

abstract：:Interaction of lectins with cell surface determinants may alter membrane properties. Using trypsinized rabbit erythrocytes as model we tested the capacity of an endogenous lectin in this respect. Galectin-1 is a member of an adhesion/growth-regulatory family known to interact for example with ganglioside GM(1) and als...

journal_title：FEBS letters

authors： Gupta RK,Pande AH,Gulla KC,Gabius HJ,Hajela K

更新日期：2006-03-06 00:00:00

abstract：:Incubation of cholinergic synaptic vesicles purified from the electric organ of Torpedo ocellata with radiolabeled [D-Ala2, D-Leu5]-enkephalin results in its accumulation in the vesicles. Kinetic analysis of the initial rates of uptake reveals temperature-dependent saturation kinetics which are best fitted by a single...

journal_title：FEBS letters

authors： Day NC,Wien D,Michaelson DM

更新日期：1985-04-08 00:00:00

abstract：:Xanthine oxidase was decreased 2- to 10-fold in all examined rat hepatomas irrespective of the malignancy; growth rate and degrees of histological differentiation of the neoplasms. The affinity to substrate (KM=6-8 muM) and the pH optimum (8.0) of the liver and hepatoma enzymes were the same. The reprogramming of gene...

journal_title：FEBS letters

authors： Prajda N,Weber G

更新日期：1975-11-15 00:00:00

abstract：:In bovine adrenocortical cells, the fatty acyl chains of the phospholipids have been identified in the membranes of the different cell compartments: plasma membranes, Golgi complex and coated vesicle membranes. An increase in the total number of unsaturation in the fatty acid is demonstrated in the coated vesicle memb...

journal_title：FEBS letters

authors： de Paillerets C,Bomsel M,Weintraub H,Pépin D,Alfsen A

更新日期：1987-07-13 00:00:00