A proteomic screen reveals novel Fas ligand interacting proteins within nervous system Schwann cells.

abstract：:Canine aortic strip studies revealed insensitivity of angiotensin II (AII)-induced aortic contraction to inhibition by the non-peptide antagonist DuP753 (pKB = 6.7 +/- 0.1). In order to determine the origin of this phenomenon we cloned the canine homologue of the AT1 AII receptor. Expression of this cDNA in COS-7 cell...

journal_title：FEBS letters

authors： Burns L,Clark KL,Bradley J,Robertson MJ,Clark AJ

更新日期：1994-04-25 00:00:00

abstract：:The tropomyosin-related kinase A (TrkA) receptor and its ligand, nerve growth factor (NGF), play crucial roles in the development and function of the nervous system. NGF is believed to activate TrkA by bridging two TrkA monomers, leading to TrkA transphosphorylation. However, here we show that the majority of TrkA rec...

journal_title：FEBS letters

authors： Shen J,Maruyama IN

更新日期：2011-01-21 00:00:00

abstract：:We have identified and purified a DNA binding protein which specifically binds to a segment of the mouse alpha 2 (I) collagen promoter between -420 and -399 bp upstream of the start of transcription. Purification included heparin-agarose and sequence-specific DNA-affinity chromatography, followed by SDS-PAGE and renat...

journal_title：FEBS letters

authors： Hatamochi A,de Crombrugghe B,Krieg T

更新日期：1993-08-02 00:00:00

abstract：:Incubation of isolated rat liver nuclei with ATP, NAD+, and micromolar Ca2+ concentrations of various metal ions resulted in extensive DNA hydrolysis. Half-maximal activity occurred with 1.0 microM Ca2+ added, and saturation of the process was observed with 10 microM Ca2+. The Ca2+ (10 microM)-activated DNA fragmentat...

journal_title：FEBS letters

authors： Yamaguchi M,Sakurai T

更新日期：1991-02-25 00:00:00

abstract：:HSP73, the constitutive form of heat-shock protein 70, has been implicated in the translocation of preproteins across the mitochondrial membranes, being required for maintaining mitochondrial preproteins in an import competent conformation. Here we report that highly purified mitochondrial outer membranes contain a pr...

journal_title：FEBS letters

authors： Lithgow T,Ryan M,Anderson RL,Høj PB,Hoogenraad NJ

更新日期：1993-10-18 00:00:00

abstract：:We have studied the inhibition by a phosphorothioate oligodeoxynucleotide (17PScap) of cDNA synthesis performed by either avian or murine reverse transcriptase. Three different mechanisms of inhibition were identified: at low concentrations (< 100 nM), the cleavage of the RNA template by the retroviral RNase H at the ...

journal_title：FEBS letters

authors： Boiziau C,Moreau S,Toulmé JJ

更新日期：1994-03-07 00:00:00

abstract：:We applied 1D homonuclear Hartmann-Hahn (1D-HOHAHA) and difference NOE experiments to determine the chemical structure of Forssman's antigen, a glycolipid purified from sheep red blood cells. The subspectra corresponding to the individual sugar components were extracted from overlapping proton resonances by selective ...

journal_title：FEBS letters

authors： Inagaki F,Kodama C,Suzuki M,Suzuki A

更新日期：1987-07-13 00:00:00

abstract：:Isoenzymes of creatine kinase (CK, EC 2.7.3.2) in guinea-pig smooth, cardiac and skeletal muscles as well as in brain were analyzed by cellulose acetate electrophoresis and FPLC gel permeation chromatography. In crude tissue extracts of smooth muscles brain type BB-CK and the hybrid form MB-CK were detected, but in en...

journal_title：FEBS letters

authors： Ishida Y,Wyss M,Hemmer W,Wallimann T

更新日期：1991-05-20 00:00:00

abstract：:32P-Labelled washed rabbit platelets were incubated with 0.6 nM platelet activating factor (PAF-acether), giving a full aggregation and release response within 30-60 s. The major phospholipid changes observed under these conditions were: (1) An increased labelling of phosphatidic acid (PA) within 10 s and of phosphati...

journal_title：FEBS letters

authors： Mauco G,Chap H,Douste-Blazy L

更新日期：1983-03-21 00:00:00

abstract：:The Hippo-Warts pathway defines a novel signaling cascade involved in organ size control and tumor suppression. However, the developmental function of this pathway is less understood. Here we report that the Caenorhabditis elegans homolog of Warts, Ce-wts-1, plays important roles during worm development. The null alle...

journal_title：FEBS letters

authors： Cai Q,Wang W,Gao Y,Yang Y,Zhu Z,Fan Q

更新日期：2009-10-06 00:00:00

abstract：:Thrombomodulin (TM) is a single-transmembrane glycoprotein receptor for thrombin, which is best known as a cofactor for thrombin-mediated activation of anticoagulant protein C. C-type lectin-like domain (CTLD) of TM has distinct coagulation/fibrinolysis-independent anti-inflammatory properties. Here we found anti-infl...

journal_title：FEBS letters

authors： Jin H,Yang X,Liu K,Gu Q,Xu X

更新日期：2011-11-04 00:00:00

abstract：:Protein synthesis in cell-free systems of rat liver and kidney decreases markedly with age. Examination of activity changes of the different steps revealed for both types of organs that reduced binding of aminoacyl-tRNA to ribosomes and reduced peptidyl transfer might be of major importance for the decrease in overall...

journal_title：FEBS letters

authors： Gabius HJ,Engelhardt R,Deerberg F,Cramer F

更新日期：1983-08-22 00:00:00

abstract：:As a conserved non-coding RNA gene, transcripts of MALAT-1 localize predominately in the nucleus. However in G2/M cell cycle phase, MALAT-1 transcripts were surprisingly found to translocate from the nucleus into the cytoplasm. Investigation also found that in this process MALAT-1 interacts with an abundant nuclear fa...

journal_title：FEBS letters

authors： Yang F,Yi F,Han X,Du Q,Liang Z

更新日期：2013-10-01 00:00:00

abstract：:We investigated the signaling pathways participating in hyperosmotic regulation of the human taurine transporter (TAUT) by using specific inhibitors of various intracellular signaling molecules. Among them, the specific inhibitor of calcium/calmodulin-dependent protein kinase II (Ca(2+)/CaM kinase II) completely repre...

journal_title：FEBS letters

authors： Satsu H,Manabe M,Shimizu M

更新日期：2004-07-02 00:00:00

abstract：:Researchers still have great difficulty in isolating individual ribosomal proteins from the ribosome in quantities high enough for structural research. To this end, when studying protein S7, we created an E. coli overproducer of the recombinant protein S7 of Thermus thermophilus. The vector for expression was pQE-32 h...

journal_title：FEBS letters

authors： Karginov AV,Karginova OA,Spiridonova VA,Kopylov AM

更新日期：1995-08-07 00:00:00

abstract：:Methane monooxygenase (MMO) catalyses the biological transformation of methane to methanol at a binuclear iron site. Guided by the three-dimensional structure of the R2 protein of E. coli ribonucleotide reductase (RNR), we have aligned the sequences of two different MMOs with the sequences of the iron coordinating fou...

journal_title：FEBS letters

authors： Nordlund P,Dalton H,Eklund H

更新日期：1992-08-03 00:00:00

abstract：:Most geckos are nocturnal forms and possess rod retinas, but some diurnal genera have pure-cone retinas. We isolated cDNAs encoding the diurnal gecko opsins, dg1 and dg2, similar to nocturnal gecko P521 and P467, respectively. Despite the large morphological differences between the diurnal and nocturnal gecko photorec...

journal_title：FEBS letters

authors： Taniguchi Y,Hisatomi O,Yoshida M,Tokunaga F

更新日期：1999-02-19 00:00:00

abstract：:The bacterial toxin VacA produced by H. pylori induces large vacuoles in several types of cultured cells such as HeLa cells or gastric cells. To determine the mechanism of vacuolation induced by this toxin we employed several inhibitors of membrane trafficking and endocytosis. The development of vacuolation induced by...

journal_title：FEBS letters

authors： Seto K,Hayashi-Kuwabara Y,Yoneta T,Suda H,Tamaki H

更新日期：1998-07-24 00:00:00

abstract：:Elongation factor Tu (EF-Tu) is the most abundant protein in prokaryotic cells. Its general function in protein biosynthesis is well established. It is a member of the large family of G-proteins, all of which bind guanosine phosphates (GDP or GTP) as cofactors. In its active GTP bound state EF-Tu binds aminoacylated t...

journal_title：FEBS letters

authors： Nissen P,Reshetnikova L,Siboska G,Polekhina G,Thirup S,Kjeldgaard M,Clark BF,Nyborg J

更新日期：1994-12-19 00:00:00

abstract：:Transgenically introduced laminin (LN) alpha1 chain prevents muscular dystrophy in LNalpha2 chain deficient mice. We now report increased integrin alpha7Bbeta1D synthesis in dystrophic LNalpha2 chain deficient muscle. Yet, immunofluorescence demonstrated a reduced expression of integrin alpha7B subunit at the sarcolem...

journal_title：FEBS letters

authors： Gawlik KI,Mayer U,Blomberg K,Sonnenberg A,Ekblom P,Durbeej M

更新日期：2006-03-20 00:00:00

abstract：:We have isolated cDNA molecules encoding a protein with the characteristic sequence elements that are conserved between the catalytic domains of protein kinases. This protein is apparently a serine/threonine kinase and is most closely related to the amino-terminal half of the ribosomal protein S6 kinase II first chara...

journal_title：FEBS letters

authors： Harmann B,Kilimann MW

更新日期：1990-10-29 00:00:00

abstract：:Chronic myelogenous leukemia (CML) is associated with the presence of the Philadelphia chromosome, which is generated by the reciprocal translocation of chromosomes 9 and 22. In the case of L6 (b2a2) mRNA, it is difficult to cleave the abnormal mRNA specifically because the mRNA includes no sequences that can be cleav...

journal_title：FEBS letters

authors： Hamada M,Kuwabara T,Warashina M,Nakayama A,Taira K

更新日期：1999-11-12 00:00:00

abstract：:Mammalian pancreatic phospholipase A2 (PLA2-I) has its specific receptor through which PLA2-I induces a variety of biological responses. In this study, a fundamental relationship between the enzymatic and the receptor-binding activities of PLA2-I was investigated. The specific binding of PLA2-I to the receptor was fou...

journal_title：FEBS letters

authors： Ishizaki J,Kishino J,Teraoka H,Ohara O,Arita H

更新日期：1993-06-21 00:00:00

abstract：:The structure of the Fab fragment of the monoclonal antibody MAK 33 (kappa/IgG1) at pH 2 was characterized. Spectroscopic and kinetic analysis revealed a molten globule-like state, characterized by elements of secondary structure but less defined tertiary contacts than in the native state. However, some aromatic side ...

journal_title：FEBS letters

authors： Lilie H,Buchner J

更新日期：1995-03-27 00:00:00

abstract：:Boar semen contains a seminal plasma motility inhibitor (SPMI) that blocks the motility of demembranated-reactivated spermatozoa as well as of intact spermatozoa. In this paper, we describe the primary structure of SPMI, the coding of boar SPMI cDNA gene and its expression in various porcine tissues. Nucleotide sequen...

journal_title：FEBS letters

authors： Iwamoto T,Hiroaki H,Furuichi Y,Wada K,Satoh M,Satoh M,Osada T,Gagnon C

更新日期：1995-07-24 00:00:00

abstract：:Disruption of the gene pgil of Saccharomyces cerevisiae, which codes for phosphoglucose isomerase, results in a dramatic increase in the amount of intracellular glycogen in early exponential cultures. The level of glucose 6-phosphate was much higher in mutant than in wild-type cells. Phosphorylase a activity and the s...

journal_title：FEBS letters

authors： Corominas J,Clotet J,Fernández-Bañares I,Boles E,Zimmermann FK,Guinovart JJ,Ariño J

更新日期：1992-09-28 00:00:00

abstract：:Drug carriers such as liposomes are not readily transported across cellular barriers that constitute epithelia. However, certain epithelia (rabbit yolk sac endoderm and enterocytes of suckling rat gut proximal small intestine) are well known to transcytose maternal IgG by Fc receptor-mediated endocytic events. We have...

journal_title：FEBS letters

authors： Patel HM,Wild AE

更新日期：1988-07-18 00:00:00

abstract：:It is known that the DNA binding Runt domain of the AML1/Runx1 transcription factor coordinates Cl(-) ions. In this paper we have determined Cl(-) binding affinities of AML1 by (35)Cl nuclear magnetic resonance (NMR) linewidth analysis. The Runt domain binds Cl(-) with a dissociation constant (K(d,Cl)) of 34 mM. If CB...

journal_title：FEBS letters

authors： Wolf-Watz M,Bäckström S,Grundström T,Sauer U,Härd T

更新日期：2001-01-12 00:00:00

abstract：:Phospholipase C(gamma)2 (PLCgamma2) is a phospholipid-converting enzyme which, upon receptor stimulation, is activated within membrane-bound signalling complexes. In contrast to the highly ubiquitous PLCgamma1, PLCgamma2 is expressed predominantly in B-lymphocytes. Associated with antigen-coupling receptors it is acti...

journal_title：FEBS letters

authors： Kang JS,Kohlhuber F,Hug H,Marmé D,Eick D,Ueffing M

更新日期：1996-12-09 00:00:00

abstract：:Autophagy is a bulk degradation process conserved among eukaryotes. In macro-autophagy, autophagosomes sequester cytoplasmic components and deliver their contents to lysosomes/vacuoles. Autophagosome formation requires the conjugation of Atg8, a ubiquitin-like protein, to phosphatidylethanolamine (PE). Here we report ...

journal_title：FEBS letters

authors： Hanada T,Satomi Y,Takao T,Ohsumi Y

更新日期：2009-04-02 00:00:00