Abstract:
:Thrombomodulin (TM) is a single-transmembrane glycoprotein receptor for thrombin, which is best known as a cofactor for thrombin-mediated activation of anticoagulant protein C. C-type lectin-like domain (CTLD) of TM has distinct coagulation/fibrinolysis-independent anti-inflammatory properties. Here we found anti-inflammatory effects of a novel peptide (GC31) from CTLD of TM in endotoxin-induced uveitis, which was characterized by a reduction of leukocyte counts, protein concentration, tumor necrosis factor (TNF)-α and monocyte chemoattractant protein (MCP)-1 levels in aqueous humor. Through in vitro experiments, we further found that GC31 suppressed TNF-α and interleukin (IL)-6 expressions in lipopolysaccharide (LPS)-stimulated macrophage-like RAW264.7 cells and interrupted LPS-induced nuclear factor-κB (NF-κB) activation. These data indicate a beneficial role of peptide GC31 in preventing intraocular inflammatory response, especially uveitis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Jin H,Yang X,Liu K,Gu Q,Xu Xdoi
10.1016/j.febslet.2011.10.002subject
Has Abstractpub_date
2011-11-04 00:00:00pages
3457-64issue
21eissn
0014-5793issn
1873-3468pii
S0014-5793(11)00740-Xjournal_volume
585pub_type
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