Transforming growth factor alpha protects against Fas-mediated liver apoptosis in mice.


:The Fas/Fas ligand interaction plays a crucial role in various liver diseases, and administration of agonistic anti-Fas antibody to mice causes massive hepatic apoptosis and fulminant hepatic failure. Several growth factors have recently been found to function in preventing apoptosis. In this study, we demonstrated that overexpression of transforming growth factor alpha (TGFalpha) has a dramatic protective effect on Fas-mediated hepatic apoptosis at the biochemical and histological levels. Moreover, 85.7% (six out of seven) of TGFalpha transgenic mice survived the lethal liver damage, whereas all wild-type mice died. Expression of Bcl-xL, an anti-apoptotic protein, was greatly increased in the transgenic mice. Taken together, our findings suggest that TGFalpha protects against Fas-mediated liver apoptosis in vivo and up-regulation of Bcl-xL may participate in protective effect of TGFalpha.




FEBS letters


Kanda D,Takagi H,Toyoda M,Horiguchi N,Nakajima H,Otsuka T,Mori M





Has Abstract


2002-05-22 00:00:00














  • Phorbol-ester-induced phosphorylation of the beta 2-adrenergic receptor decreases its coupling to Gs.

    abstract::Phorbol-esters have been shown to modulate the beta-adrenergic-stimulated adenylyl cyclase in a number of cell lines. Here, using site directed mutagenesis, we investigate the role of the beta 2-adrenergic receptor phosphorylation by protein kinase C in this regulatory process. Mutation of the serine-261, -262, -344 a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bouvier M,Guilbault N,Bonin H

    更新日期:1991-02-25 00:00:00

  • 2-Arachidonoylglycerol, an endogenous cannabinoid receptor agonist: identification as one of the major species of monoacylglycerols in various rat tissues, and evidence for its generation through CA2+-dependent and -independent mechanisms.

    abstract::The molecular species compositions of monoacylglycerols obtained from various rat tissues were examined by reverse-phase high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses. We confirmed that 2-arachidonoylglycerol, an endogenous cannabinoid receptor agonist, is one ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kondo S,Kondo H,Nakane S,Kodaka T,Tokumura A,Waku K,Sugiura T

    更新日期:1998-06-12 00:00:00

  • The algorithm of estimation of the Km values for primers of various structure and length in the polymerization reaction catalyzed by Klenow fragment of DNA polymerase I from E. coli.

    abstract::DNA synthesis at primers d(pT)n, d(pA)n, d(pC)n, and d(pG)n in the presence of corresponding complementary templates and at hetero-oligoprimers complementary to M13 phage DNA was investigated. The values of both -log Km and log Vmax increased linearly if homo-oligoprimers contained less than 10 nucleotides. The length...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nevinsky GA,Nemudraya AV,Levina AS,Khomov VV

    更新日期:1989-11-20 00:00:00

  • Ligand-receptor interactions in the nicotinic acetylcholine receptor probed using multiple substitutions at conserved tyrosines on the alpha subunit.

    abstract::Affinity labeling studies have identified several conserved tyrosine residues in the alpha subunit of the nicotinic acetylcholine receptor (alpha Y93, alpha Y190, and alpha Y198) as being in or near the ligand binding site. Mutagenesis studies from several laboratories have shown that substitution of phenylalanine for...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Aylwin ML,White MM

    更新日期:1994-07-25 00:00:00

  • Primary structure of matrilin-3, a new member of a family of extracellular matrix proteins related to cartilage matrix protein (matrilin-1) and von Willebrand factor.

    abstract::A mouse cDNA encoding for matrilin-3, the third member of the novel matrilin family of extracellular matrix proteins, was cloned. The protein precursor of 481 amino acids consists of a putative signal peptide, a short positively charged sequence, a single vWFA-like domain followed by four epidermal growth factor-like ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wagener R,Kobbe B,Paulsson M

    更新日期:1997-08-11 00:00:00

  • Crystal structure of human nicotinamide mononucleotide adenylyltransferase in complex with NMN.

    abstract::The final step in the biosynthesis of nicotinamide-adenine dinucleotide, a major coenzyme in cellular redox reactions and involved in intracellular signaling, is catalyzed by the enzyme nicotinamide mononucleotide adenylyltransferase (NMNAT). The X-ray structure of human NMNAT in complex with nicotinamide mononucleoti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Werner E,Ziegler M,Lerner F,Schweiger M,Heinemann U

    更新日期:2002-04-10 00:00:00

  • Phosphorylation of the L-type calcium channel beta subunit is involved in beta-adrenergic signal transduction in canine myocardium.

    abstract::Cyclic AMP-mediated phosphorylation of calcium channel subunits was studied in vitro and in vivo in preparations from dog heart. Calcium channels in native cardiac membranes were phosphorylated by cAMP-dependent protein kinase (PKA) solubilized with digitonin and subsequently immunoprecipitated using a polyclonal anti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Haase H,Karczewski P,Beckert R,Krause EG

    更新日期:1993-12-06 00:00:00

  • A cis-dominant cyclic nucleotide-dependent regulatory domain in the 3'-untranslated region of Na(+)/glucose cotransporter (SGLT1) mRNA.

    abstract::A 122 nt uridine-rich sequence (URE) in the Na(+)/glucose cotransporter (SGLT1) mRNA 3'-untranslated region is critical for cAMP-dependent message stabilization. Its function was investigated in LLC-PK(1) cells stably expressing beta-globin reporter transcripts. Insertion of the SGLT1 URE downstream from an unrelated ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Loflin P,Lever JE

    更新日期:2001-03-16 00:00:00

  • Interactions of the HIV-1 reverse transcriptase 'AZT-resistant' mutant with substrates and AZT-TP.

    abstract::To investigate the biochemical basis of the HIV-1 resistance to AZT we obtained the RT mutant containing four amino acid substitutions by an oligonucleotide-directed mutagenesis technique. Enzymatic properties of the wild type and mutant RTs were compared. 'AZT-resistant' mutations in RT were shown to be associated wi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pokholok DK,Gudima SO,Yesipov DS,Dobrynin VN,Rechinsky VO,Kochetkov SN

    更新日期:1993-07-05 00:00:00

  • Antibodies directed against guanylic acid and guanosine are nucleolus specific probes.

    abstract::Purified antibodies against guanylic acid and guanosine binding to RNA at guanosine residues were used to probe human lymphocyte preparations by indirect immunofluorescence. Neither antibody gave any banding pattern with metaphase chromosomes but both showed binding to specific sites in the interphase nuclei. Evidence...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Iswari S,Krishnamurthy DS,Jacob TM

    更新日期:1985-09-23 00:00:00

  • Hydrocortisone inhibits prostaglandin production but not arachidonic acid release from cultured macrophages.

    abstract::We have investigated the action of hydrocortisosone on arachidonic acid mobilisation in cultures of mouse peritoneal macrophages, mouse L929 cells and the mouse macrophage-like cell line RAW264. Hydrocortisone inhibits both arachidonic acid release and prostaglandin production by L929 cells. However, prostaglandin pro...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wood JN,Coote PR,Rhodes J

    更新日期:1984-08-20 00:00:00

  • A proform of secretin with high secretin-like bioactivity.

    abstract::A variant form of the heptacosapeptide amide secretin, with C-terminal -Val-Gly-Lys-Arg instead of valine amide, has been isolated from porcine upper intestinal tissue. Unexpectedly, this triacontapeptide exhibited a substantially higher bioactivity than the heptacosapeptide amide. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gafvelin G,Carlquist M,Mutt V

    更新日期:1985-05-20 00:00:00

  • Low molecular mass GTP-binding proteins of adrenal chromaffin cells are present on the secretory granule.

    abstract::Adrenal medullary homogenates and chromaffin granule membranes were separated by SDS-polyacrylamide gel electrophoresis and GTP-binding proteins detected using [alpha-32P]GTP binding to nitrocellulose blots. Four GTP-binding polypeptides of 24, 22, 20 and 18 kDa were routinely found in medullary homogenates and all we...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Burgoyne RD,Morgan A

    更新日期:1989-03-13 00:00:00

  • Analysis of the black-eyed pea trypsin and chymotrypsin inhibitor-alpha-chymotrypsin complex.

    abstract::The black-eyed pea trypsin and chymotrypsin inhibitor (BTCI) is a member of the Bowman-Birk protease inhibitor (BBI) family. The three-dimensional model of the BTCI-chymotrypsin complex was built based on the homology to Bowman-Birk inhibitors with known structures. An extensive theoretical and experimental study of t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: de Freitas SM,de Mello LV,da Silva MC,Vriend G,Neshich G,Ventura MM

    更新日期:1997-06-09 00:00:00

  • Time dependence of activation of muscle AMP-aminohydrolase by substrate and potassium ion.

    abstract::The kinetics of AMP-aminohydrolase, which under steady state conditions shows a typical sigmoid dependence of initial velocities versus substrate concentration, have been examined by rapid mixing methods. Using this technique it was observed that when substrate or substrate plus activator (K(+)) were mixed with enzyme...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Suelter CH,Kovacs AL,Antonini E

    更新日期:1968-11-01 00:00:00

  • Promiscuous actions of small molecule inhibitors of the protein kinase D-class IIa HDAC axis in striated muscle.

    abstract::PKD-mediated phosphorylation of class IIa HDACs frees the MEF2 transcription factor to activate genes that govern muscle differentiation and growth. Studies of the regulation and function of this signaling axis have involved MC1568 and Gö-6976, which are small molecule inhibitors of class IIa HDAC and PKD catalytic ac...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lemon DD,Harrison BC,Horn TR,Stratton MS,Ferguson BS,Wempe MF,McKinsey TA

    更新日期:2015-04-28 00:00:00

  • MicroRNA-320a inhibits cell proliferation, migration and invasion by targeting BMI-1 in nasopharyngeal carcinoma.

    abstract::In the present study, we investigated the roles and molecular mechanisms of miR-320a in human nasopharyngeal carcinoma (NPC). miR-320a expression was strongly reduced in NPC tissues and cell lines. Overexpression of miR-320a significantly suppressed NPC cell growth, migration, invasion and tumor growth in a xenograft ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Qi X,Li J,Zhou C,Lv C,Tian M

    更新日期:2014-10-16 00:00:00

  • An interface point-mutation variant of triosephosphate isomerase is compactly folded and monomeric at low protein concentrations.

    abstract::Wild-type trypanosomal triosephosphate isomerase (wtTIM) is a very tight dimer. The interface residue His-47 of wtTIM has been mutated into an asparagine. Ultracentrifugation data show that this variant (H47N) only dimerises at protein concentrations above 3 mg/ml. H47N has been characterised at a protein concentratio...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Borchert TV,Zeelen JP,Schliebs W,Callens M,Minke W,Jaenicke R,Wierenga RK

    更新日期:1995-07-03 00:00:00

  • X-linked inhibitor of apoptosis protein mediates neddylation by itself but does not function as a NEDD8-E3 ligase for caspase-7.

    abstract::X-linked inhibitor of apoptosis protein (XIAP) is a potent antagonist of caspases, and functions as a ubiquitin-E3 ligase by itself and for caspases. Recently, NEDD8, a ubiquitin-like modifier, has been suggested to be used for modification of caspase-7 mediated by XIAP. However, it is not clear whether caspase-7 is a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nagano T,Hashimoto T,Nakashima A,Kikkawa U,Kamada S

    更新日期:2012-06-04 00:00:00

  • Up-regulation of lactosylceramide synthase in MDR1 overexpressing human liver tumour cells.

    abstract::HepG2 cells, stably transfected with MDR1 cDNA, encoding the P-glycoprotein multidrug resistance efflux pump, display an altered sphingolipid composition compared to control cells, stably transfected with empty vector. The MDR1 overexpressing cells display a approximately 3-fold increased level of lactosylceramide and...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hummel I,Klappe K,Kok JW

    更新日期:2005-06-20 00:00:00

  • Poly (ADP-ribose) polymerase inhibits DNA replication by human replicative DNA polymerase alpha, delta and epsilon in vitro.

    abstract::The influence of poly (ADP-ribose) polymerase (PARP) and poly ADP-ribosylation on DNA synthesis supported by human replicative DNA polymerase (DNA pol) alpha, delta, and epsilon has been examined using the replication system containing poly(dA)4500-oligo(dT)12-18 as the template primer. PARP alone inhibited the pol ac...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Eki T

    更新日期:1994-12-19 00:00:00

  • Expression of the cell-adhesion molecule VCAM-1 by stromal cells is necessary for osteoclastogenesis.

    abstract::Osteoblastic cells have been shown to be involved in osteoclast formation through cell to cell contacts. This study was designed to examine the possible function of vascular cell adhesion molecule 1 (VCAM-1) during osteoclastogenesis. As a source for stromal cells we used the recently established mouse bone marrow str...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Feuerbach D,Feyen JH

    更新日期:1997-01-27 00:00:00

  • Enterohemolysin operon of Shiga toxin-producing Escherichia coli: a virulence function of inflammatory cytokine production from human monocytes.

    abstract::Shiga toxin-producing Escherichia coli (STEC) is associated with hemolytic uremic syndrome (HUS). Although most clinical isolates of STEC produce hemolysin (called enterohemolysin), the precise role of enterohemolysin in the pathogenesis of STEC infections is unknown. Here we demonstrated that E. coli carrying the clo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Taneike I,Zhang HM,Wakisaka-Saito N,Yamamoto T

    更新日期:2002-07-31 00:00:00

  • Regulation of DNA supercoiling in Escherichia coli: genetic basis of a compensatory mutation in DNA gyrase.

    abstract::Bacterial DNA supercoiling is controlled by balancing the supercoiling activity of DNA gyrase and the relaxing activity of DNA topoisomerase I. We have characterized the gyrB gene from a top A deletion mutant of Escherichia coli (DM800) that has a compensatory mutation in gyrB, lowering the activity of gyrase 10-fold,...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: McEachern F,Fisher LM

    更新日期:1989-08-14 00:00:00

  • Site-specific phosphorylation of villin remodels the actin cytoskeleton to regulate Sendai viral glycoprotein-mediated membrane fusion.

    abstract::Connivance of cellular factors during virus-host cell membrane fusion is poorly understood. We have recently shown that cellular villin plays an important role during membrane fusion of reconstituted Sendai virosomes with hepatocytes. Here, we employed villin-null Chinese Hamster Ovary (CHO) cells, where villin expres...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chandra S,Kumar M,Sharma NR,Sarkar DP

    更新日期:2019-08-01 00:00:00

  • Dynamic re-distribution of protein kinase D (PKD) as revealed by a GFP-PKD fusion protein: dissociation from PKD activation.

    abstract::Protein kinase D (PKD)/protein kinase Cmicro (PKCmicro, a serine/threonine protein kinase with distinct structural and enzymological properties, is rapidly activated in intact cells via PKC. The amino-terminal region of PKD contains a cysteine-rich domain (CRD) that directly binds phorbol esters with a high affinity. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Matthews S,Iglesias T,Cantrell D,Rozengurt E

    更新日期:1999-09-03 00:00:00

  • Substrate interactions in cytochrome P-450: correlation between carbon-13 nuclear magnetic resonance chemical shifts and C-O vibrational frequencies.

    abstract::13CO NMR chemical shifts and 12CO infrared stretching frequencies have been measured for cytochrome P-450cam-CO in the presence of D-camphor and of various camphor analogues. A linear correlation between both parameters delta (13C) and v(CO) was found indicating that the steric and electrostatic interactions acting on...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Legrand N,Bondon A,Simonneaux G,Jung C,Gill E

    更新日期:1995-05-08 00:00:00

  • Dominant-negative effect of mutant valosin-containing protein in aggresome formation.

    abstract::Lewy bodies (LBs) are the pathologic hallmark of Parkinson's disease. Recent studies revealed that LBs exhibit several morphologic and molecular similarities to aggresomes. Aggresomes are perinuclear aggregates representing intracellular deposits of misfolded proteins. Recently, valosin-containing protein (VCP) was on...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kitami MI,Kitami T,Nagahama M,Tagaya M,Hori S,Kakizuka A,Mizuno Y,Hattori N

    更新日期:2006-01-23 00:00:00

  • A novel subunit of axonemal dynein conserved among lower and higher eukaryotes.

    abstract::To elucidate the subunit composition of axonemal inner-arm dynein, we examined a 38 kDa protein (p38) co-purified with a Chlamydomonas inner arm subspecies, dynein d. We found it is a novel protein conserved among a variety of organisms with motile cilia and flagella. Immunoprecipitation using specific antibody verifi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yamamoto R,Yanagisawa HA,Yagi T,Kamiya R

    更新日期:2006-11-27 00:00:00

  • Verification of the interdomain contact site in the inactive monomer, and the domain-swapped fold in the active dimer of Hsp33 in solution.

    abstract::Upon dimerization by oxidation, Hsp33 functions as a molecular chaperone in prokaryotes. Previously published structures of both the inactive and active species are of doubtful relevance to the solution conformations since the inactive (reduced) crystal structure was dimeric, while the active (oxidized) species was cr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lee YS,Ryu KS,Kim SJ,Ko HS,Sim DW,Jeon YH,Kim EH,Choi WS,Won HS

    更新日期:2012-02-17 00:00:00