Abstract:
:MiR-140 is a microRNA specially involved in chondrogenesis and osteoarthritis pathogenesis. However, its transcriptional regulation and target genes in cartilage development are not fully understood. Here we detected that miR-140 was uniquely expressed in chondrocyte and suppressed by Wnt/β-catenin signalling. The miR-140 primary transcript was an intron-retained RNA co-expressed with Wwp2-C isoform, which was directly induced by Sox9 through binding to the intron 10 of Wwp2 gene. Knockdown of miR-140 in limb bud micromass cultures resulted in arrest of chondrogenic proliferation. Sp1, the activator of the cell cycle regulator p15(INK4b), was identified as a target of miR-140 in maintaining the chondrocyte proliferation. Collectively, our findings expand our understanding of the transcriptional regulation and the chondrogenic role of miR-140 in chondrogenesis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Yang J,Qin S,Yi C,Ma G,Zhu H,Zhou W,Xiong Y,Zhu X,Wang Y,He L,Guo Xdoi
10.1016/j.febslet.2011.08.013subject
Has Abstractpub_date
2011-10-03 00:00:00pages
2992-7issue
19eissn
0014-5793issn
1873-3468pii
S0014-5793(11)00603-Xjournal_volume
585pub_type
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