Identification of active site serine and histidine residues in Escherichia coli outer membrane protease OmpT.

abstract：:Mutation of Thr90 to Ala has a profound effect on bacteriorhodopsin properties. T90A shows about 20% of the proton pumping efficiency of wild type, once reconstituted into liposomes. Mutation of Thr90 influences greatly the Schiff base/Asp85 environment, as demonstrated by altered lambda(max) of 555 nm and pK(a) of As...

journal_title：FEBS letters

authors： Perálvarez A,Barnadas R,Sabés M,Querol E,Padrós E

更新日期：2001-11-23 00:00:00

abstract：:We report that the rat beta 3-adrenergic receptor (beta 3-AR) gene has an intron. The intron starts with an in-frame stop codon with the result that unspliced transcripts will encode a C-terminal truncated protein. The reported protein sequences of mouse and human beta 3-AR were both deduced from genomic DNA sequences...

journal_title：FEBS letters

authors： Bensaid M,Kaghad M,Rodriguez M,Le Fur G,Caput D

更新日期：1993-03-08 00:00:00

abstract：:The glycine-alanine (GA) repeat of the Epstein-Barr virus nuclear antigen-1 inhibits in cis ubiquitin-dependent proteolysis in mammalian cells through a yet unknown mechanism. In the present study we demonstrate that the GA repeat targets an evolutionarily conserved step in proteolysis since it can prevent the degrada...

journal_title：FEBS letters

authors： Heessen S,Dantuma NP,Tessarz P,Jellne M,Masucci MG

更新日期：2003-12-04 00:00:00

abstract：:Acetylcholine (1-10 microM) depolarized the membrane and stimulated glucose-induced bursts of electrical activity in mouse pancreatic B-cells. The acetylcholine effects were mimicked by muscarine while nicotine had no effect on membrane potential. Pirenzepine, an antagonist of the classical M1-type muscarinic receptor...

journal_title：FEBS letters

authors： Santos RM,Rojas E

更新日期：1989-06-05 00:00:00

abstract：:We propose a unified framework for the identification of functional interaction structures of biomolecular networks in a way that leads to a new experimental design procedure. In developing our approach, we have built upon previous work. Thus we begin by pointing out some of the restrictions associated with existing s...

journal_title：FEBS letters

authors： Cho KH,Choo SM,Wellstead P,Wolkenhauer O

更新日期：2005-08-15 00:00:00

abstract：:Study of the relative proportions of ribosomes and polysomes released by a standardized lysing procedure from isogenic RC(str) and RC(rel) strains of Escherichia coli shows that a 20-min period of amino acid starvation of RC(str) bacteria reduces the fraction of ribosomes recovered in polysomes to about 60% of its val...

journal_title：FEBS letters

authors： Matzura H

更新日期：1970-01-15 00:00:00

abstract：:We report the effects of anticancer drugs, inhibitors of microtubule organisation, on the mitochondrial permeability transition pore (PTP) in Ehrlich ascites tumour cells. Taxol (5-20 microM) and colchicine (100-500 microM) prevented closing of the cyclosporin A-sensitive PTP. No taxol or colchicine effects on oxidati...

journal_title：FEBS letters

authors： Evtodienko YV,Teplova VV,Sidash SS,Ichas F,Mazat JP

更新日期：1996-09-09 00:00:00

abstract：:Various properties have been evaluated for the binding to tissue culture substrata of proteolytic fragments of human plasma or cellular fibronectins containing complementary sequences from the individual and alternatively spliced chains, since related fragments are known to yield differing adhesive responses from cell...

journal_title：FEBS letters

authors： Lewandowska K,Kaetzel CS,Zardi L,Culp LA

更新日期：1988-09-12 00:00:00

abstract：:A variety of mutations leading to amino acid substitutions have been described in the transthyretin gene in association with different familial amyloidoses and have been implicated to be involved in the pathogenesis of amyloid deposits. However, there has been disagreement whether or not a transthyretin mutation is pr...

journal_title：FEBS letters

authors： Christmanson L,Betsholtz C,Gustavsson A,Johansson B,Sletten K,Westermark P

更新日期：1991-04-09 00:00:00

abstract：:The expression of the cytochrome P4501A1 gene, CYP1A1, is induced by e.g. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) mainly by transcriptional mechanisms. The inducers mediate their effect upon binding and activation of the aryl hydrocarbon receptor (AHR) transcription-factor complex. Utilizing chimeric CYP1A1/CAT con...

journal_title：FEBS letters

authors： Jørgensen EC,Autrup H

更新日期：1995-05-29 00:00:00

abstract：:Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydroge...

journal_title：FEBS letters

authors： Shin EY,Min DS,Shin JC,Shin KS,Hyun MS,Ha KS,Kim HS,Ahn HY,Kim EG

更新日期：2001-11-16 00:00:00

abstract：:The class A Ca2+ channel alpha 1 subunit (alpha 1A) was expressed in Xenopus oocytes alone or in combination with the beta 1b, beta 2a, beta 3, or beta 4 subunit. Analysis of voltage-dependent activation and inactivation in the presence of 1.8 mM external Ca2+ showed an hyperpolarising shift of both relations when com...

journal_title：FEBS letters

authors： Cens T,Mangoni ME,Nargeot J,Charnet P

更新日期：1996-08-12 00:00:00

abstract：:Rat glutathione transferase 8-8 is one of the less abundant cytosolic glutathione transferases, accounting for approx. 1% of the total activity with 1-chloro-2,4-dinitrobenzene in liver. The enzyme is eluted at pH 6.3 upon chromatofocusing and has so far been identified in liver, kidney, lung and testis. Characteristi...

journal_title：FEBS letters

authors： Jensson H,Guthenberg C,Alin P,Mannervik B

更新日期：1986-07-28 00:00:00

abstract：:Bacterial fructosyltransferase enzymes belonging to glycoside hydrolase family 68 (GH68) are not known to require a metal cofactor. Here, we show that Ca2+ ions play an important structural role in the Lactobacillus reuteri 121 levansucrase (Lev) and inulosucrase (Inu) enzymes. Analysis of the Bacillus subtilis Lev 3D...

journal_title：FEBS letters

authors： Ozimek LK,Euverink GJ,van der Maarel MJ,Dijkhuizen L

更新日期：2005-02-14 00:00:00

abstract：:Incubation of human thrombocytes in the presence of [3H]palmitic acid leads to incorporation of this fatty acid into the alpha subunit of Gs as described [Linder et al., Proc. Natl. Acad. Sci. USA 90 (1993) 3675-3679; Degtyarev et al., Biochemistry 32 (1993) 8057-8061] but also into the catalyst of adenylyl cyclase wh...

journal_title：FEBS letters

authors： Mollner S,Beck K,Pfeuffer T

更新日期：1995-09-11 00:00:00

abstract：:Escherichia coli evolves H2 via multiple [Ni-Fe]-hydrogenases (Hyd). This activity under hyper- and hypo-osmotic stress was investigated with mutants lacking different Hyd enzymes during glycerol fermentation. Inhibitory effects of hypo-stress on H2 production was stronger at pH 6.5 in wild type and mutants except fhl...

journal_title：FEBS letters

authors： Trchounian K,Trchounian A

更新日期：2013-11-01 00:00:00

abstract：:The existence of the internal promoter Pi in the 16 S/23 S intergenic spacers of the rRNA operons of an eubacterium Escherichia coli and archaebacterium Halobacterium halobium is proposed. The possible functional significance of these promoters is discussed. ...

journal_title：FEBS letters

authors： Mankin AS,Skripkin EA,Kagramanova VK

更新日期：1987-07-27 00:00:00

abstract：:Capsaicin, a major ingredient of hot pepper, is considered to exhibit anti-inflammatory properties. Our previous study demonstrated that capsaicin inhibited the production of pro-inflammatory mediators through NF-kappaB inactivation in LPS-stimulated macrophages. In order to further clarify the mechanism underlying th...

journal_title：FEBS letters

authors： Park JY,Kawada T,Han IS,Kim BS,Goto T,Takahashi N,Fushiki T,Kurata T,Yu R

更新日期：2004-08-13 00:00:00

abstract：:Piericidin is a potent inhibitor of the mitochondrial and bacterial type I NADH-ubiquinone oxidoreductases (Complex I) and is considered to bind at or close to the ubiquinone binding site(s) of the enzyme. Piericidin-resistant mutants of the bacterium Rhodobacter capsulatus have been isolated and the present work demo...

journal_title：FEBS letters

authors： Darrouzet E,Issartel JP,Lunardi J,Dupuis A

更新日期：1998-07-10 00:00:00

abstract：:Some members of the Pax (paired box) and Six (sine oculis homeobox) gene families function as components of a gene regulatory network controlling eye development. To investigate the early evolution of the genetic interaction between Pax and Six genes, we identified and sequenced members of the Pax and Six gene familie...

journal_title：FEBS letters

authors： Hoshiyama D,Iwabe N,Miyata T

更新日期：2007-04-17 00:00:00

abstract：:VirG, PhoB, and OmpR are positive regulators of the virulence, phosphate, and osmolarity regulons, respectively. Their target genes are attended with one or more significant blocks of similar 6-bp sequences in their regulatory regions. The distance between the block(s) and transcriptional start site varies with the ta...

journal_title：FEBS letters

authors： Aoyama T,Oka A

更新日期：1990-04-09 00:00:00

abstract：:The interactions of DNA duplexes with 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), a potent mutacarcinogen isolated from tryptophan pyrolysate, have been studied using CD spectroscopy. The results are that (a) the spectral change of B-form DNA caused by the interaction with Trp-P-1 is biphasic, i.e. the enla...

journal_title：FEBS letters

authors： Inohara T,Tarui M,Doi M,Inoue M,Ishida T

更新日期：1993-06-21 00:00:00

abstract：:The organization of the U1snRNP-specific A protein (34 kDa) has been analyzed by 12 and 16 A thiol-reversible chemical cross-linking and Western blotting. A-containing cross-linked complexes had molecular masses of 43, 47, 56, 62, 67, 105 and 125 kDa. None of these complexes could be cross-linked following ribonucleas...

journal_title：FEBS letters

authors： Boix J,Habets WJ,van Venrooij WJ,Smith HC

更新日期：1990-10-15 00:00:00

abstract：:In order to improve our understanding of membrane protein solubilization by sodium dodecylsulphate, sarcoplasmic reticulum vesicles have been treated with this surfactant at different detergent: protein mole ratios. Effects on Ca2(+)-ATPase activity, membrane protein solubilization, and protein conformation have been ...

journal_title：FEBS letters

authors： Prado A,Muga A,Castresana J,Goñi FM,Arrondo JL

更新日期：1990-09-03 00:00:00

abstract：:Apoptosis repressor with caspase recruitment domain is expressed at high levels in brain and myogenic tissues, consistent with a role to inhibit apoptosis in the terminally differentiated cells. Expression of ARC in cancers is not known. In this study, we reported that ARC was highly expressed in various non-myogenic ...

journal_title：FEBS letters

authors： Wang M,Qanungo S,Crow MT,Watanabe M,Nieminen AL

更新日期：2005-04-25 00:00:00

abstract：:Lipid analyses were carried out on transplantable murine Friend leukemia cell tumors, 6 h after intratumoral administration of tumor necrosis factor-alpha (TNF). The levels of the major phospholipid classes were uniformly decreased to about 70% of control values; free fatty acids were increased to about 170%; diacylgl...

journal_title：FEBS letters

authors： Bravo E,Carpinelli G,Proietti E,Belardelli F,Cantafora A,Podo F

更新日期：1990-01-29 00:00:00

abstract：:We studied the effect of point mutation within the putative 11th transmembrane domain (TM11) of the Na(+)/H(+) exchanger NHE1 on the plasma membrane expression. Of the 19 mutants tested, two mutants (Tyr454 or Arg458 replaced by Cys) were retained in the endoplasmic reticulum. Interestingly, Y454C was expressed on the...

journal_title：FEBS letters

authors： Wakabayashi S,Pang T,Su X,Shigekawa M

更新日期：2000-12-29 00:00:00

abstract：:The enantiomers of U50,488, ligands highly selective for kappa-opioid receptors, have been prepared by a refined procedure and their optical purity demonstrated. The absolute configuration of (+)-trans-2-pyrrolidinyl-N-methylcyclohexylamine, a chemically versatile intermediate for synthesis of analogs of kappa-opioid ...

journal_title：FEBS letters

authors： DeCosta B,George C,Rothman RB,Jacobson AE,Rice KC

更新日期：1987-11-02 00:00:00

abstract：:The addition of 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) to peroxisomes decreased the production of acid-soluble radioactivity formed by beta-oxidation of [1-(14)C]arachidonate due to substrate removal by esterification into the acceptor. This peroxisomal-associated acyl-CoA:1-acyl-GPC acyltransferase activity ...

journal_title：FEBS letters

authors： Baykousheva SP,Luthria DL,Sprecher H

更新日期：1995-06-26 00:00:00

abstract：:Syntabulin is a microtubule-associated protein that mediates anterograde transport of vesicles to neuronal processes. Here, we found that syntabulin was expressed in mouse pancreas and insulin-secreting β-cells, and that it partially co-localized with microtubule and insulin-containing granules. The association of syn...

journal_title：FEBS letters

authors： Ying Y,Li L,Cao W,Yan D,Zeng Q,Kong X,Lu L,Yan M,Xu X,Qu J,Su Q,Ma X

更新日期：2012-10-19 00:00:00