A common mechanism of transcriptional activation by the three positive regulators, VirG, PhoB, and OmpR.

abstract：:The distribution of flux control coefficients of a glycolytic system in starved rat liver has been determined. The flux control coefficient profile in starved conditions is compared with normal fed conditions showing that in the former phosphofructokinase enhances more than 2-fold its flux control coefficient while gl...

journal_title：FEBS letters

authors： Torres NV,Mateo F,Meléndez-Hevia E

更新日期：1988-06-06 00:00:00

abstract：:Complexes between the urokinase-type plasminogen activator (uPA) and its type-2 inhibitor (PAI-2) are bound by a cell-surface receptor for uPA and rapidly cleaved into two fragments of 70 and 22 kDa. The 70-kDa fragment contains the active site of uPA and PAI-2, while the 22-kDa species was identified as the amino ter...

journal_title：FEBS letters

authors： Ragno P,Montuori N,Vassalli JD,Rossi G

更新日期：1993-06-01 00:00:00

abstract：:The reduction of methyl-coenzyme M (CH3SCoM) to methane in methanogenic bacteria is dependent on component B (N-7-mercaptoheptanoyl-O-phospho-L-threonine, HSHTP). We report here that S-methyl-component B (N-7-(methylthio)heptanoyl-O-phospho-L-threonine, CH3SHTP) can substitute for neither CH3SCoM nor HSHTP in the meth...

journal_title：FEBS letters

authors： Ellermann J,Kobelt A,Pfaltz A,Thauer RK

更新日期：1987-08-17 00:00:00

abstract：:In dog-fish spermatid nuclei two intermediate proteins S1 and S2 replace histones before the setting down of protamines. These spermatid-specific proteins were isolated by carboxymethyl-cellulose chromatography and purified by high pressure liquid chromatography. S1 and S2 are characterized by a high content of basic ...

journal_title：FEBS letters

authors： Chauviere M,Laine B,Sautiere P,Chevaillier P

更新日期：1983-02-21 00:00:00

abstract：:Silver and gold compounds were tested as potential inhibitors of aquaporins of plant- and human origin. Silver as AgNO(3) or silver sulfadiazine inhibited with high potency (EC(50) 1-10 microM) the water permeability of the peribacteroid membrane from soybean (containing Nodulin 26), the water permeability of plasma m...

journal_title：FEBS letters

authors： Niemietz CM,Tyerman SD

更新日期：2002-11-20 00:00:00

abstract：:Replacing the essential Cys-149 by a selenocysteine into the active site of phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPDH) from Bacillus stearothermophilus leads to a selenoGAPDH that mimics a selenoperoxidase activity. Saturation kinetics were observed with cumenyl and tert-butyl hydroperoxides, wit...

journal_title：FEBS letters

authors： Boschi-Muller S,Muller S,Van Dorsselaer A,Böck A,Branlant G

更新日期：1998-11-20 00:00:00

abstract：:The glucagon-like peptide 1 (7-37)/(7-36) amide (GLP-1) receptor belongs to a new subclass of seven transmembrane domain, G-protein coupled receptors comprising several receptors for peptide hormones. The receptors of this family share many common motifs including a relatively large N-terminal extracellular domain. Th...

journal_title：FEBS letters

authors： Wilmen A,Göke B,Göke R

更新日期：1996-11-25 00:00:00

abstract：:Acylphosphatase (AcP) activity in prokaryotes was classically attributed to some aspecific acid phosphatases. We identified an open reading frame for a putative AcP in the b0968 Escherichia coli gene and purified the recombinant enzyme after checking by RT-PCR that it was indeed expressed. EcoAcP has a predicted typic...

journal_title：FEBS letters

authors： Ramazzotti M,Parrini C,Stefani M,Manao G,Degl'Innocenti D

更新日期：2006-12-22 00:00:00

abstract：:The full-length cDNA encoding the human calbindin-D9k (CaBP-9k) has been cloned using reverse transcription/PCR methodology with rat- and bovine-derived primers and intestinal RNA. A core product, and both a 5' and 3' product encompassing the full-length cDNA were obtained. The clones include coding region for 79 amin...

journal_title：FEBS letters

authors： Jeung EB,Krisinger J,Dann JL,Leung PC

更新日期：1992-07-28 00:00:00

abstract：:A new assay for the calcium channel activity of annexin V was developed. The calcium-sensitive fluorescence indicator, fura-2, was incorporated into large unilamellar vesicles (LUV). After establishing a calcium gradient across the liposomal membranes, native or mutated annexin V was added. The resulting calcium influ...

journal_title：FEBS letters

authors： Berendes R,Burger A,Voges D,Demange P,Huber R

更新日期：1993-02-08 00:00:00

abstract：:The bacteriophytochrome Agp1 was reconstituted with a locked 5Zs-biliverdin in which the C(4)=C(5) and C(5)-C(6) bonds of the methine bridge between rings A and B are fixed in the Z and syn configuration/conformation, respectively. In Agp1-5Zs the photoconversion proceeds via the Lumi-R intermediate to Meta-R(A), but ...

journal_title：FEBS letters

authors： Seibeck S,Borucki B,Otto H,Inomata K,Khawn H,Kinoshita H,Michael N,Lamparter T,Heyn MP

更新日期：2007-11-27 00:00:00

abstract：:Transgenic Escherichia coli expressing pyrroloquinoline-quinone (PQQ) synthase gene from Deinococcus radiodurans showed superior survival during Rose Bengal induced oxidative stress. Such cells showed significantly low levels of protein carbonylation as compared to non-transgenic control. In vitro, PQQ reacted with re...

journal_title：FEBS letters

authors： Misra HS,Khairnar NP,Barik A,Indira Priyadarsini K,Mohan H,Apte SK

更新日期：2004-12-03 00:00:00

abstract：:We previously obtained evidence for intrinsic aminopeptidase activity for leukotriene (LT)A4 hydrolase, an enzyme characterized to specifically catalyse the hydrolysis of LTA4 to LTB4, a chemotactic compound. From a sequence homology search between LTA4 hydrolase and several aminopeptidases, it became clear that they ...

journal_title：FEBS letters

authors： Minami M,Bito H,Ohishi N,Tsuge H,Miyano M,Mori M,Wada H,Mutoh H,Shimada S,Izumi T

更新日期：1992-09-14 00:00:00

abstract：:DNA methylation has a profound impact on the regulation of gene expression in normal cell development, and aberrant methylation has been recognized as a key factor in the pathogenesis of human diseases such as cancer. The discovery of modified nucleobases arising from 5-methylcytosine (5mC) through consecutive oxidati...

journal_title：FEBS letters

authors： Dietzsch J,Feineis D,Höbartner C

更新日期：2018-06-01 00:00:00

abstract：:Acute hyperammonemia, induced by two consecutive injections of ammonium acetate (550 and 450 mg per kg b.wt.), decreased the activity of gamma-glutamyl transpeptidase (GGT) in most brain regions of 18- and 30-day-old rats. This decrease in the brain GGT activity was more pronounced in younger than in older rats. After...

journal_title：FEBS letters

authors： Stastný F,Pitha J,Lisý V,Hilgier W,Kaucká I,Albrecht J

更新日期：1992-04-06 00:00:00

abstract：:Transfer RNAs (tRNAs) are ancient macromolecules that have evolved under various environmental pressures as adaptors in translation in all forms of life but also towards alternative structures and functions. The present knowledge on both "canonical" and "deviating" signature motifs retrieved from vertical and horizont...

journal_title：FEBS letters

authors： Pütz J,Giegé R,Florentz C

更新日期：2010-01-21 00:00:00

abstract：:Yeast tRNA(Lys)CUU is nucleus-encoded and is partially imported into the mitochondria. Another lysine isoacceptor, tRNA(Lys)SUU, is also nucleus-encoded but is not imported. These two tRNAs differ in 21 bases. We have previously localised import selectivity determinants in the anticodon arm. By in vitro import of muta...

journal_title：FEBS letters

authors： Kazakova HA,Entelis NS,Martin RP,Tarassov IA

更新日期：1999-01-15 00:00:00

abstract：:In the rat liver, transcription of the serine:pyruvate/alanine:glyoxylate aminotransferase (SPT/AGT) gene occurs from two sites, +1 and +66, in exon 1, resulting in the formation of two mRNAs, one for a precursor of mitochondrial SPT/AGT and the other for peroxisomal SPT/AGT, respectively. In this study, we attempted ...

journal_title：FEBS letters

authors： Sugiyama T,Uchida C,Oda T,Kitagawa M,Hayashi H,Ichiyama A

更新日期：2001-11-09 00:00:00

abstract：:The matrix protein M of Borna disease virus (BDV) is a constituent of the viral envelope covering the inner leaflet of the lipid bilayer. BDV-M was expressed as recombinant protein in Escherichia coli, purified to homogeneity and structurally analyzed. Recombinant M (i) forms non-covalently bound multimers with a Stok...

journal_title：FEBS letters

authors： Kraus I,Bogner E,Lilie H,Eickmann M,Garten W

更新日期：2005-05-09 00:00:00

abstract：:The polytopic transmembrane protein, Niemann-Pick C1-Like 1 (NPC1L1), is enriched in the apical membrane of small intestine absorptive enterocytes where it mediates extracellular sterol transport across the brush border membrane. It is essential for intestinal sterol absorption and is the molecular target of ezetimibe...

journal_title：FEBS letters

authors： Betters JL,Yu L

更新日期：2010-07-02 00:00:00

abstract：:We have isolated a cDNA encoding a precursor of dermorphin from the skin of Pachymedusa dacnicolor. Besides four copies of this opioid peptide, the deduced sequence also contains the genetic information for a novel peptide Tyr-Ile-Phe-His-Leu-Met-Asp-NH2. This differs from Met-deltorphin by the presence of Ile at posi...

journal_title：FEBS letters

authors： Wechselberger C,Severini C,Kreil G,Negri L

更新日期：1998-06-05 00:00:00

abstract：:The Ras homology (Rho) guanine nucleotide exchange factor p115-RhoGEF couples the alpha(13) heterotrimeric guanine nucleotide binding protein (G protein) subunit to Rho GTPase. Alpha(13) binds to a regulator of G protein signaling (RGS) domain in p115-RhoGEF, but the mechanism of alpha(13) activation of p115-RhoGEF is...

journal_title：FEBS letters

authors： Bhattacharyya R,Wedegaertner PB

更新日期：2003-04-10 00:00:00

abstract：:The pre-steady state process in the thermolysin-catalyzed hydrolysis of Cbz-Gly-Phe-Ala was observed at pH 4.5 by fluorescence stopped-flow method using Dns-Phe as a displacement probe. After the confirmation of the pre-equilibrium hypothesis for the binary interaction, the nonlinear substrate concentration dependence...

journal_title：FEBS letters

authors： Kunugi S,Yokoyama M,Sakamoto A

更新日期：1995-04-03 00:00:00

abstract：:The reaction of alpha-chymotrypsin with AcTyr-OEt and with AcTrp-OEt at pH 7.0 and 7.8 was studied over a wide range of substrate concentrations. The reaction with AcTyr-OEt at pH 7.8 was shown to be non-hyperbolic using a variety of criteria whereas those at pH 7.0 with the same substrate and at both pH values with A...

journal_title：FEBS letters

authors： Garrell J,Cuchillo CM

更新日期：1985-10-14 00:00:00

abstract：:Vpu is an 81 amino acid protein encoded by HIV-1. Its role is to amplify viral release by two mechanisms: (i) docking to CD4 with the consequence of targeting CD4 for ubiquitine-mediated degradation, and (ii) formation of ion channels to enhance particle release. The intensive research on its in vivo function, combine...

journal_title：FEBS letters

authors： Fischer WB

更新日期：2003-09-18 00:00:00

abstract：:Tropomodulin, the P-end (slow-growing end)-capping protein of the actin-tropomyosin filament, and its fragment (C20) of the C-terminal half were studied by synchrotron small-angle X-ray scattering, restoring low-resolution shapes using an ab initio shape-determining procedure. Tropomodulin is elongated (115 A long) an...

journal_title：FEBS letters

authors： Fujisawa T,Kostyukova A,Maéda Y

更新日期：2001-06-01 00:00:00

abstract：:Aging is accompanied by troubles resulting from changes in hormonal and nutritional status. Therefore, the abundance of mRNA coding for triiodothyronine (TR) and retinoic acid (RA) nuclear receptors was studied in the brain of young, adult and aged (2.5, 6 and 24 months, respectively) rats. In the brain of aged rats, ...

journal_title：FEBS letters

authors： Enderlin V,Alfos S,Pallet V,Garcin H,Azaïs-Braesco V,Jaffard R,Higueret P

更新日期：1997-08-04 00:00:00

abstract：:We show here that phosphorylation of the insulin receptor and insulin receptor substrate-1 is increased when suspended cells are replated on fibronectin. This is not due to decreased numbers of cell surface receptors, alteration of insulin binding, or stimulation of a phosphatase activity in non-adherent cells. Expres...

journal_title：FEBS letters

authors： El Annabi S,Gautier N,Baron V

更新日期：2001-11-02 00:00:00

abstract：:The effective synthesis of the envelope antigen F1 of Y. pestis in E. coli HB101 is mediated by the expression of the caf1M gene. This gene was sequenced, and the protein encoded was found to have a significant homology with the chaperone protein PapD of uropathogenic E. coli. The data presented allow one to suppose C...

journal_title：FEBS letters

authors： Galyov EE,Karlishev AV,Chernovskaya TV,Dolgikh DA,Smirnov OYu,Volkovoy KI,Abramov VM,Zav'yalov VP

更新日期：1991-07-29 00:00:00

abstract：:Acaryochloris marina is a prochlorophyte-like cyanobacterium containing both phycobilins and chlorophyll d as light harvesting pigments. We show that the chlorophyll d light harvesting system, composed of Pcb proteins, functionally associates with the photosystem II (PSII) reaction center (RC) core to form a giant sup...

journal_title：FEBS letters

authors： Chen M,Bibby TS,Nield J,Larkum AW,Barber J

更新日期：2005-02-14 00:00:00