The effect of ammonia and pH on brain gamma-glutamyl transpeptidase in young rats.

abstract：:The BChla-containing B880-complex (core-complex) of Rhodopseudomonas marina (Rhodospirillaceae) was isolated with a new purification method. The isolation of the B880-complex was performed by solubilisation of the photosynthetic membranes with the detergent LDAO and subsequent fractionated ammonium-sulfate precipitati...

journal_title：FEBS letters

authors： Meckenstock RU,Brunisholz RA,Zuber H

更新日期：1992-10-19 00:00:00

abstract：:Bispecific miniantibodies were constructed by genetically fusing the C(H)1 domain of an IgG1 to the C-terminus of a single-chain Fv fragment (scFv-425), specific for the EGF receptor, and fusing the C(L) domain of a kappa light chain to the C-terminus of a scFv specific for CD2 (scFv-M1). An efficient dicistronic gene...

journal_title：FEBS letters

authors： Müller KM,Arndt KM,Strittmatter W,Plückthun A

更新日期：1998-01-30 00:00:00

abstract：:Recombinant Plasmodium falciparum glyoxalase I (PfGlx I) was characterized as monomeric Zn(2+)-containing enzyme of 44 kDa. The K(M) value of the methylglyoxal-glutathione adduct is 77+/-15 microM, the k(cat) value being 4000 min(-1) at 25 degrees C and pH 7.0. PfGlx I consists of two halves, each of which is homologo...

journal_title：FEBS letters

authors： Iozef R,Rahlfs S,Chang T,Schirmer H,Becker K

更新日期：2003-11-20 00:00:00

abstract：:Pleckstrin homology (PH) domains are protein modules found in proteins involved in many cellular processes. The majority of PH domain-containing proteins require membrane association for their function. It has been shown that most PH domains interact directly with the cell membrane by binding to phosphoinositides with...

journal_title：FEBS letters

authors： Maffucci T,Falasca M

更新日期：2001-10-12 00:00:00

abstract：:This study reports for the first time (a) the longitudinal profile of the transmembrane potential (mDeltapsi) of single mitochondria using a Nernstian fluorescent probe and (b) the distribution of mDeltapsi fluctuations of mitochondria undergoing permanent depolarization. Our findings show that (1) mitochondria in dif...

journal_title：FEBS letters

authors： Diaz G,Falchi AM,Gremo F,Isola R,Diana A

更新日期：2000-06-23 00:00:00

abstract：:Insulin resistant states are associated with an increase in the beta cell mass and also high levels of circulating insulin. Ultimately the beta cells undergo a failure that leads to diabetes. At this stage, a question arises if those persistent high levels of circulating insulin may contribute to beta cell damage. To ...

journal_title：FEBS letters

authors： Guillen C,Bartolomé A,Nevado C,Benito M

更新日期：2008-11-26 00:00:00

abstract：:Upon in vitro translation of mRNAs from slow (soleus) muscles of the mouse a hitherto undescribed translation product has been detected that was absent from fast skeletal muscles and was termed p19/6.8 according to its position in 2-dimensional gels. mRNA for p19/6.8 was also found in the ventricle of the heart. p19/6...

journal_title：FEBS letters

authors： Kluxen FW

更新日期：1988-03-28 00:00:00

abstract：:Here, we used a reductant, N-acetyl-L-cysteine (NAC), to investigate the redox-sensitive step(s) in the signalling pathway from the tumor necrosis factor (TNF) receptor to nuclear factor kappaB (NF-kappaB). We found that NAC suppressed NF-kappaB activation triggered by TNF or by overexpression of either the TNF recept...

journal_title：FEBS letters

authors： Oka S,Kamata H,Kamata K,Yagisawa H,Hirata H

更新日期：2000-04-28 00:00:00

abstract：:HepG2 cells, stably transfected with MDR1 cDNA, encoding the P-glycoprotein multidrug resistance efflux pump, display an altered sphingolipid composition compared to control cells, stably transfected with empty vector. The MDR1 overexpressing cells display a approximately 3-fold increased level of lactosylceramide and...

journal_title：FEBS letters

authors： Hummel I,Klappe K,Kok JW

更新日期：2005-06-20 00:00:00

abstract：:Human high- and low-Mr kininogens were shown to be potent inhibitors of cysteine proteinases such as cathepsin L and papain (Ki = 17-48 pM). A strong immunological cross-reaction between the kininogens and low-Mr alpha-cysteine proteinase inhibitor from human plasma was found. Comparison of partial amino acid sequence...

journal_title：FEBS letters

authors： Müller-Esterl W,Fritz H,Machleidt W,Ritonja A,Brzin J,Kotnik M,Turk V,Kellermann J,Lottspeich F

更新日期：1985-03-25 00:00:00

abstract：:The bacterial toxin VacA produced by H. pylori induces large vacuoles in several types of cultured cells such as HeLa cells or gastric cells. To determine the mechanism of vacuolation induced by this toxin we employed several inhibitors of membrane trafficking and endocytosis. The development of vacuolation induced by...

journal_title：FEBS letters

authors： Seto K,Hayashi-Kuwabara Y,Yoneta T,Suda H,Tamaki H

更新日期：1998-07-24 00:00:00

abstract：:We investigated the effect of hydroxyl substituted chalcone (1a) and some chalcone analogues (1b-d) on isolated rat liver mitochondria to gain new insights into the cytotoxic mechanism of these compounds. We observed an inhibitory effect on phosphorylation and the partial uncoupling of compounds 1a and 1d. Increased r...

journal_title：FEBS letters

authors： Guzy J,Vasková-Kubálková J,Rozmer Z,Fodor K,Mareková M,Poskrobová M,Perjési P

更新日期：2010-02-05 00:00:00

abstract：:The macromolecular diversity and concentrations in the fluid phase of cytoplasm constitute conditions necessary and sufficient for aqueous phase separation. Consequences of phase separation in cytoplasm, including its 'compartmentation', are inferred from analogies with the physicochemical properties of aqueous two-ph...

journal_title：FEBS letters

authors： Walter H,Brooks DE

更新日期：1995-03-20 00:00:00

abstract：:Complete [1H]NMR resonance assignments are presented for the second of the three zinc fingers from SW15 as it appears in both 1- and 2-finger constructs. Signals from finger 2 are unaffected by the presence or absence of finger 1, showing that the protein has a modular construction. The structure of finger 2 comprises...

journal_title：FEBS letters

authors： Neuhaus D,Nakaseko Y,Nagai K,Klug A

更新日期：1990-03-26 00:00:00

abstract：:In Ruminococcus albus, 4-O-β-D-mannosyl-D-glucose phosphorylase (RaMP1) and β-(1,4)-mannooligosaccharide phosphorylase (RaMP2) belong to two subfamilies of glycoside hydrolase family 130. The two enzymes phosphorolyze β-mannosidic linkages at the nonreducing ends of their substrates, and have substantially diverse sub...

journal_title：FEBS letters

authors： Ye Y,Saburi W,Odaka R,Kato K,Sakurai N,Komoda K,Nishimoto M,Kitaoka M,Mori H,Yao M

更新日期：2016-03-01 00:00:00

abstract：:Using a prostate-specific antigen cDNA as a hybridization probe, clones containing the kallikrein genes encoding prostate-specific antigen, human glandular kallikrein-1 and pancreas/kidney kallikrein were isolated from a human genomic library. Clones containing the prostate-specific antigen gene and the human glandula...

journal_title：FEBS letters

authors： Riegman PH,Vlietstra RJ,Klaassen P,van der Korput JA,Geurts van Kessel A,Romijn JC,Trapman J

更新日期：1989-04-10 00:00:00

abstract：:The Alanine Serine Cysteine Transporter 2 (ASCT2) is involved in balancing the intracellular amino acid pool. This function is allowed by the antiport mechanism and the asymmetric specificity towards different neutral amino acids, distinctive of this transporter. In the present work, the interaction of the putative su...

journal_title：FEBS letters

authors： Scalise M,Pochini L,Pingitore P,Hedfalk K,Indiveri C

更新日期：2015-11-30 00:00:00

abstract：:A cell-free system derived from carrot cell cytosol extract has been developed for reassembling nuclear structure around the added demembranated sperm chromatin of Xenopus. Morphological evidence suggests that reassembled nuclei display the typical characteristics of normal eukaryotic nuclei, such as double-layered nu...

journal_title：FEBS letters

authors： Zhao Y,Liu X,Wu M,Tao W,Zhai Z

更新日期：2000-09-01 00:00:00

abstract：:When bottromycin A2 was added to an in vitro protein synthesis system carried out by naturally occurring polysomes, it inhibited protein synthesis effectively. Examination of the 3 steps of peptide chain elongation revealed that the binding of aminoacyl-tRNA to the polyribosomes was inhibited by bottromycin A2. In con...

journal_title：FEBS letters

authors： Otaka T,Kaji A

更新日期：1983-03-07 00:00:00

abstract：:Acid extracts of rat pituitary neuro-intermediate lobes have been shown by ion-exchange chromatography and radio-immunoassay to contain predominantly the inactive derivatives of beta-endorphin, alpha, N-acetyl beta-endorphin 1-27 and alpha, N-acetyl beta-endorphin 1-26; the biologically active form, beta-endorphin 1-3...

journal_title：FEBS letters

authors： Ham J,Smyth DG

更新日期：1984-10-01 00:00:00

abstract：:The Rad51 gene of Saccharomyces cerevisiae is required for genetic recombination and recombinational repair of DNA strand breaks. In higher eukaryotes Rad51 is essential for embryonic development, and is involved in cell proliferation and DNA repair. Here we show that human Rad51 (HsRad51) is proteolytically cleaved d...

journal_title：FEBS letters

authors： Flygare J,Armstrong RC,Wennborg A,Orsan S,Hellgren D

更新日期：1998-05-08 00:00:00

abstract：:Muscarinic K+ channels (IK(ACh)) in native atrial myocytes are activated by betagamma subunits of pertussis toxin (Ptx)-sensitive heterotrimeric G proteins coupled to different receptors. betagamma subunits of Ptx-insensitive Gs, coupled to beta-adrenergic receptors, do not activate native IK(ACh). In atrial myocytes ...

journal_title：FEBS letters

authors： Bender K,Wellner-Kienitz MC,Meyer T,Pott L

更新日期：1998-11-13 00:00:00

abstract：:Conserved residues of the proteolytic domain of Escherichia coli protease Lon, putative members of the classic catalytic triad (H665, H667, D676, and D743) were identified by comparison of amino acid sequences of Lon proteases. Mutant enzymes containing substitutions D676N, D743N, H665Y, and H667Y were obtained by sit...

journal_title：FEBS letters

authors： Starkova NN,Koroleva EP,Rumsh LD,Ginodman LM,Rotanova TV

更新日期：1998-01-30 00:00:00

abstract：:A colostral proline-rich polypeptide complex (PRP) consisting of over 30 peptides shows beneficial effects in Alzheimer's disease (AD) patients when administered in the form of sublinqual tablets called Colostrinin. The aim of the present studies was to investigate whether nanopeptide fragment of PRP (NP) - one of the...

journal_title：FEBS letters

authors： Janusz M,Woszczyna M,Lisowski M,Kubis A,Macała J,Gotszalk T,Lisowski J

更新日期：2009-01-05 00:00:00

abstract：:Although neurokinin A (NKA), a tachykinin peptide with sequence homology to substance P (SP), is a weak competitor of radiolabeled SP binding to the NK-1 receptor (NK-1R), more recent direct binding studies using radiolabeled NKA have demonstrated an unexpected high-affinity interaction with this receptor. To document...

journal_title：FEBS letters

authors： Bremer AA,Leeman SE,Boyd ND

更新日期：2000-12-01 00:00:00

abstract：:It has previously been suggested that the mammary cell could produce prolactin (PRL). This hypothesis was investigated by incubation with [35S]methionine-cysteine followed by SDS-PAGE, immunoblotting and autoradiography of immunoprecipitated PRL, and by electron microscopic analysis after incubation without or with cy...

journal_title：FEBS letters

authors： Lkhider M,Delpal S,Le Provost F,Ollivier-Bousquet M

更新日期：1997-01-20 00:00:00

abstract：:We have analyzed the glycosaminoglycans exposed on the surface of endothelial cells cultured in vitro. The cells were labelled with [35S]sodium sulfate. Chondroitin sulfate, heparan sulfate and dermatan sulfate are the main sulfated glycosaminoglycans present on the cell surface. They are synthesized by endothelial ce...

journal_title：FEBS letters

authors： Cavari S,Vannucchi S

更新日期：1993-05-24 00:00:00

abstract：:Two free radical scavengers, bioflavonoid rutin and the copper-rutin complex Cu(Rut)Cl2, inhibited lucigenin-amplified chemiluminescence and lipid peroxidation in rat liver microsomes, Cu(Rut)Cl2 being a 5-9 times more efficient inhibitor than rutin. The enhanced inhibitory activity of Cu(Rut)Cl2 was due to the presen...

journal_title：FEBS letters

authors： Afanas'ev IB,Ostrachovich EA,Korkina LG

更新日期：1998-03-27 00:00:00

abstract：:WT1 was isolated as a tumor suppressor gene of Wilms tumor. However, high expression of WT1 correlates with poor prognosis in acute leukemia. In addition suppression of WT1 expression by WT1 anti-sense oligonucleotide inhibits proliferation of leukemia cells, suggesting that WT1 is important for their proliferation. T...

journal_title：FEBS letters

authors： Murata Y,Kudoh T,Sugiyama H,Toyoshima K,Akiyama T

更新日期：1997-06-02 00:00:00

abstract：:The eye lens protein and chaperonin, alpha-crystallin, was studied by differential scanning microcalorimetry, spectroscopy and size exclusion chromatography. The thermal transition of alpha-crystallin proceeds at Ttrs = 59.8 +/- 0.6 degrees C with an enthalpy change of delta H = 336 +/- 9 kJ per mol subunit. Disagreem...

journal_title：FEBS letters

authors： Gesierich U,Pfeil W

更新日期：1996-09-16 00:00:00