Abstract:
:WT1 was isolated as a tumor suppressor gene of Wilms tumor. However, high expression of WT1 correlates with poor prognosis in acute leukemia. In addition suppression of WT1 expression by WT1 anti-sense oligonucleotide inhibits proliferation of leukemia cells, suggesting that WT1 is important for their proliferation. To further elucidate the biological significance of WT1 in leukemic cell growth, we overexpressed exogenous WT1 in murine M1 myeloblastic leukemia cells using the isopropyl-beta-D-thiogalactoside (IPTG)-controlled expression system. We found that induction of one splicing variant of WT1 [WT1-17AA(+)-KTS(-)] in M1 cells induces cell cycle arrest and apoptotic cell death. These results suggest that the role of WT1 is different depending on the type of leukemia cell in which it is expressed.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Murata Y,Kudoh T,Sugiyama H,Toyoshima K,Akiyama Tdoi
10.1016/s0014-5793(97)00477-8subject
Has Abstractpub_date
1997-06-02 00:00:00pages
41-5issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(97)00477-8journal_volume
409pub_type
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