Abstract:
:Lipoprotein MtsA is a critical component of MtsABC responsible for iron binding and transport in the Gram-positive bacterium Streptococcus pyogenes. The present collective experimental data establish that Fe(2+) is the primary binding ion for MtsA under optimal physiologically relevant conditions. The binding affinities of MtsA to metal ions are Fe(2+)>Fe(3+)>Cu(2+)>Mn(2+)>Zn(2+). We report for the first time that bicarbonate is required as a synergistic anion for stable ferrous binding to MtsA, similar to the iron binding in human transferrin. This work provides valuable information, which helps to understand iron metabolism in bacteria, and creates a basis for developing strategies to suppress bacterial infection.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sun X,Ge R,Chiu JF,Sun H,He QYdoi
10.1016/j.febslet.2008.03.020subject
Has Abstractpub_date
2008-04-16 00:00:00pages
1351-4issue
9eissn
0014-5793issn
1873-3468pii
S0014-5793(08)00249-4journal_volume
582pub_type
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