Abstract:
:Plant G proteins of the ROP/RAC family regulate cellular processes including cytoskeletal rearrangement in polar growth. Activation of the ROP molecular switch is triggered by guanine nucleotide exchange factors. Plant-specific RopGEFs are exclusively active on ROPs despite their high homology to animal Rho proteins. Based on a sequence comparison of ROPs vs. animal Rho proteins together with structural data on distinct ROPs, we identified unique substrate determinants of RopGEF specificity by mutational analysis: asparagine 68 next to switch II, arginine 76 of a putative phosphorylation motif and the Rho insert are essential for substrate recognition by RopGEFs. These data also provide first evidence for a function of the Rho insert in interactions with GEFs.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Fricke I,Berken Adoi
10.1016/j.febslet.2008.12.008subject
Has Abstractpub_date
2009-01-05 00:00:00pages
75-80issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(08)00993-9journal_volume
583pub_type
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