Abstract:
:In HCT116 colorectal cancer cells, HeLa cervical cancer cells and HuH-7 hepatoma cells, miR-223 is expressed at a low level. Through infection with lentivirus containing miR-223 precursor, miR-223 [corrected] was overexpressed in all these cells. Interestingly, the expression levels of FOXO1 mRNA and protein, and phosphorylation levels became significantly lower than those of their control. FOXO1 was down-regulated mainly in the cytoplasm, while the nuclear FOXO1 level became relatively high compared to the cytoplasm. As the unphosphorylated active form of FOXO1 increased in the cells, cyclin D1/p21/p27 were up-regulated at either mRNA or protein level. Proliferation of the cells was also greatly inhibited when miR-223 was over-expressed. Therein, our data suggest that miR-223 regulates FOXO1 expression and cell proliferation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Wu L,Li H,Jia CY,Cheng W,Yu M,Peng M,Zhu Y,Zhao Q,Dong YW,Shao K,Wu A,Wu XZdoi
10.1016/j.febslet.2012.02.050subject
Has Abstractpub_date
2012-04-05 00:00:00pages
1038-43issue
7eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00174-3journal_volume
586pub_type
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