Abstract:
:In this paper we introduce monobromobimane, a thiol reagent, as a selective blocker of the recently identified dithiol whose oxidation-reduction status modifies voltage sensing by the mitochondrial permeability transition pore, a cyclosporin A-sensitive channel. Monobromobimane does not inhibit the phosphate carrier, nor does it interfere with Ca2+ transport, energy coupling or ATP production and transport. We show that monobromobimane selectively prevents the shift in pore gating potential caused by some dithiol oxidants or crosslinkers but not by increasing [Ca2+], allowing a clear distinction of the pore agonists which act at this site.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Costantini P,Chernyak BV,Petronilli V,Bernardi Pdoi
10.1016/0014-5793(95)00256-9subject
Has Abstractpub_date
1995-04-03 00:00:00pages
239-42issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(95)00256-9journal_volume
362pub_type
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