Abstract:
:Hepatitis C virus (HCV) replication and pathogenesis involve both virus-encoded proteins and cellular factors. In our study, we showed that NS5B, the HCV RNA-dependent RNA polymerase, interacted with M2 type pyruvate kinase (M2PK) but not L type pyruvate kinase. We confirmed the interaction by GST pull down, coimmunoprecipitation and confocal immunofluorescence analysis in cells with transient expression of NS5B and M2PK as well as in a HCV replicon-bearing cell line. Furthermore shRNA which specifically down-regulated M2PK expression could inhibit the replication of HCV in HCV replicon 9B cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Wu X,Zhou Y,Zhang K,Liu Q,Guo Ddoi
10.1016/j.febslet.2008.05.033subject
Has Abstractpub_date
2008-06-25 00:00:00pages
2155-60issue
15eissn
0014-5793issn
1873-3468pii
S0014-5793(08)00457-2journal_volume
582pub_type
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